Genetic similarity of Pseudomonas syringae pv. tomato strains showing various virulence
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Research Institute of Vegetable Crops, Department of Genetics, Breeding and Biotechnology Pomologiczna 18, 96-100 Skierniewice, Poland
Research Institute of Pomology and Floriculture, 96-100 Skierniewice
Corresponding author
Elżbieta U. Kozik
Research Institute of Vegetable Crops, Department of Genetics, Breeding and Biotechnology Pomologiczna 18, 96-100 Skierniewice, Poland
Journal of Plant Protection Research 2006;46(4):325-333
Bacterial speck of tomato caused by Pseudomonas syringae pv. tomato appeared to be recently the most important disease on tomato in Poland. The genetic relationships among four Polish strains of race 0 P. syringae pv. tomato of different origin, isolated from tomato plants, were examined by RAPD and PCR-RFLP techniques. Amplification of bacterial DNA using 33 primers with RAPD technique showed, that similarity of strains expressed by the Nei-Li coefficient was very high (above 0.8). Next, the restriction analysis of amplified region ITS with the use of 5 endonucleases revealed, that profiles obtained from electrophoretic separation of DNA fragments were also very similar. On the basis of those analyses it was concluded that all strains tested constituted a closely related group. However, they showed various level of virulence as was demonstrated on the inoculated leaves of tomato plants growing in the greenhouse.
The authors have declared that no conflict of interests exist.
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