ORIGINAL ARTICLE
Growth inhibition of Fusarium verticillioides (Sacc.) Nirenberg by isolates of Trichoderma pseudokoningii strains from maize plant parts and its rhizosphere
1
Department of Biological Sciences, Olabisi Onabanjo University, PMB 2002,
Ago-Iwoye, Ogun State, Nigeria
2
CSREES, USDA, Room 3153 Waterfront Centre, 1400 Independence Avenue,
Washington, DC 20250-2220, USA
3
Department of Botany and Microbiology, University of Ibadan, Ibadan, Oyo State,
Nigeria
4
International Institute of Tropical Agriculture, c/o L.W. Lambourn (UK) Ltd,
Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, UK
Corresponding author
Ayodele Adegboyega Sobowale
Department of Biological Sciences, Olabisi Onabanjo University, PMB 2002, Ago-Iwoye, Ogun State, Nigeria
Department of Biological Sciences, Olabisi Onabanjo University, PMB 2002, Ago-Iwoye, Ogun State, Nigeria
Journal of Plant Protection Research 2005;45(4):249-265
KEYWORDS
TOPICS
ABSTRACT
Ability of five strains of Trichoderma pseudokoningii (antagonists) to suppress radial growth of Fusarium verticillioides (Sacc.) Nirenberg (=
Fusarium moniliforme Sheldon) was examined invitro These were T. pseudokoningii strai n1 (IMI 380933), strai n2 (IMI 380937), strai n3 (IMI 3809 39), strai n4 (IMI 380940) a nd strai n5 (IMI 380941). Each strain was paired with pathogen by inoculating at opposite ends of 9 cm petri plates using three pairing methods. Gradings were assigned to varied
growth inhibition of pathogen by antagonists and analysed using GLM procedure (SAS). Growth suppressio nof F. verticillioides by all strains of
T. pseudokoningii was significantly different (R 2 =0.98, p=0.05) from control in all pairing methods. It differed significantly (p>0.0003) among the strains in all pairing methods. Growth suppression also differed significantly among (p>0.0001) and within (p>0.018) pairing methods. Growth suppression was best when antagonists were inoculated before pathogen. Suppression mechanisms include mycoparasitism and competition for space and nutrients. T. pseudokoningii strains 3 and 4 had the best (p=0.05) growth suppressio nof F. verticillioides and could be used as biocontrol agents for endophytic F. verticillioides in maize plant. This experiment was conducted in the search for resedent microorganisms that might be capable of checking F. verticillioides withi nmaize plant by competitive exclusion in subsequent experiment
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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