Molecular cloning and characterization of pearl millet polyphenol oxidase and its role in defense against downy mildew
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Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysuru, Karnataka, India
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-11-17
Acceptance date: 2019-08-22
Online publication date: 2019-10-02
Corresponding author
Niranjan-Raj Sathyanarayana   

Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri, Mysuru, Karnataka, India
Journal of Plant Protection Research 2019;59(3):423-427
Polyphenol oxidase partial gene PG-PPO was cloned and characterized from Pennisetum glaucum (pearl millet) which showed 42% identity to a PPO sequence isolated from wheat at the region of Copper B with a score of 40 and e-value of 2.8. Multiple sequence alignment results revealed similarity to polyphenol oxidase (PPO) sequences from wheat, trifolium, lettuce, apricot, tobacco, tomato, pokeweed, apple, grape and poplar especially at the Copper B region of PPO. The 395 bp pearl millet PPO sequence was AT rich (53.3%) and contained the highly conserved amino acids of histidine-rich copper binding sites similar to PPO sequences from other crops. Results also indicated that PPO in pearl millet exists in multi copy. The role of the isolated PPO gene during pearl millet-downy mildew interaction was analyzed and the results showed significantly higher and rapid accumulation of PPO mRNAs in resistant pearl millet seedlings inoculated with Sclerospora graminicola in comparison to the susceptible control, demonstrating that the PPO plays a prominent role in pearl millet defense against pathogens, particularly downy mildew pathogen.
The authors have declared that no conflict of interests exist.
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