Molecular detection and comparison of Gaeumannomyces graminis var. tritici isolates originating from wheat and rye
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The August Cieszkowski Agricultural University, Department of Phytopathology Dąbrowskiego 159, 60-594 Poznań, Poland
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Lidia Irzykowska
The August Cieszkowski Agricultural University, Department of Phytopathology Dąbrowskiego 159, 60-594 Poznań, Poland
Journal of Plant Protection Research 2007;47(3):299-308
Gaeumannomyces graminis is an etiologic agent of take-all, economically important disease of cereals worldwide. A polymerase chain reaction with variety-specific primers was successfully used for detection of G. graminis var. tritici in plant tissue. Obtained results showed that this diagnostic method is a very sensitive and useful tool for detection of the pathogen even before disease symptoms arise. DNA polymorphism revealed by RAPD-PCR with three arbitrary primers was suitable for assessing genetic variation among Ggt isolates originating from wheat and rye.
The authors have declared that no conflict of interests exist.
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