Preparation of new biofungicides using antagonistic bacteria and mineral compounds for controlling cotton seedling damping-off disease
More details
Hide details
Department of Environmental Sciences, Graduate School of the Environment and Energy Islamic Azad University-Science and Research Branch,Tehran, Iran
Department of Plant Pathology, Iranian Research Institute of Plant Protection, Tehran, Iran
Department of Environment, Faculty of Natural Resources, Tehran University, Karaj, Iran
Department of Environmental Management, Graduate School of the Environment and Energy Islamic Azad University-Science and Research Branch,Tehran, Iran
Department of Environmental Engineering, Faculty of Civil Engineering, KNT Univ ersity of Technology, Tehran, Iran
Corresponding author
Soheil Sobhan Ardakani
Department of Environmental Sciences, Graduate School of the Environment and Energy Islamic Azad University-Science and Research Branch,Tehran, Iran No 27, Farabord Building, North Allameh Street, Saàdat Abad, P.O. Code. 19978-53111, Tehran, Iran,
Journal of Plant Protection Research 2009;49(1):49-55
The overuse of chemical pesticides in agriculture has caused serious environmental problems and thus the demand for safer pesticides is increasing. One alternative is microbial pesticides that suppress plant pathogens via their microbial activities. As microbial pesticides are eco-friendly products, in this study we prepared four biological fungicides using two isolates of Pseudomonas fluorescens that included a talc-based powder and bentonite-based powder as mineral carriers. Then we evaluated the efficacy of these products in controlling cotton seedlings, damping-off, a fungal disease caused by Rhizoctonia solani at four intervals of 15, 30, 45 and 60 days after sowing the cotton seeds under greenhouse conditions. The results of greenhouse experiment on application of biofungicides showed that the efficacy of Bentonite-B 1 treatment to control R. solani was promising as it increased the number of healthy seedlings 3.42 to 3.57 – fold and was much more effective than thecarboxin/thiram fungicide in all stages.
The authors have declared that no conflict of interests exist.
Atkinson D., Berta G., Hooker J.E. 1994. Impact of mycorrhizal colonization root architecture. Root longevity and the formation of growth regulators p. 89–99. In: “Impact of Arbuscular Mycorrhizas on Sustainable Agriculture” (S. Gianinazzi., H. Schuepp, eds.). Natural Ecosystems, Birkhauser-Verlag, Basel.
Aziz N.H., El-fouly M.Z., El-Essawy A.A., Khalef M.A. 1997. Influence of bean seedling root exudates on the rhizosphere colonization by T. lignorum for the control of Rhizoctonia solani. Bot. Bull. Acad. Sin. 38: 33–39.
Bacon C.W., Yates I.E., Hinton D.M., Meredith F. 2001. Biological control of Fusarium moniliforme in maize. Environ Health Perspec. 109: 325–332.
Becker O.J., Schwinn F.J. 1993. Control of soil borne pathogens with living bacteria and fungi: Status and outlook. Pesticide Sci. 37: 355–363.
Bharathi R., Vivekananthan R., Harish S., Ramanathan A., Samiyappan R. 2004. Rhizobacteria-based bio-formulations for the management of fruit rot infection in chilies. Crop Protection 23: 835–843.
Borowicz V.A. 2001. Do arbuscular mycorrhizal fungi alter plantpathogen relations. Ecology 82: 3057–3068.
Cameco M., Santamaria C., Temprano F., Rodrguez-Navarro D.N., Daza A. 2001. Co-inoculation with Bacillussp. CECT 450 improves nodulation in Phaseolus vulgaris L. Can. J. Microbiol. 47: 1058–1062.
Chen C., Belanger R.R., Benhamou N., Paullitz., TC. 2000. Defence enzymes induced in cucumber roots by treatment with plant-growth promoting rhizobacteria (PGPR). Physiol. Mol. Plant Pathol. 56: 13–23.
Chung W.C., Huang J.W., Huang H.C. 2005. Formulation of a soil biofungicide for control of damping-off of Chinese cabbage (Brassica chinensis) caused by Rhizoctonia solani. J. Biol. Control 32: 287–294.
Collins D.P., Jacobsen B. 2003. Optimizing a Bacillus subtilis isolate for biological control of sugar beet Cercospora leaf spot. Biol. Control 26: 153–161.
Cook R., Baker K.F. 1983. The Nature and Practice of Biological Control of Plant Pathogens. The APS. St. Paul. Minnesota, 539 pp.
Duffy B.K., Simon A., Weller D.M. 1996. Comination of Tricoderma koningii with fluorescent pseudomonas for control take-all on wheat. Phytopathology 86: 188–194.
Emmert E.A.B., Handelsman J. 1999. Biological of plant disease: a (Gram) positive perspective. FEMS Microbiol. Letters 171: 1–9.
Ghonim M.I. 1999. Induction of systemic resistance against Fusarium wilt in tomato with the biocontrol agent Bacillus subtilis. Bull. Faculty. Agricul. University of Cairo 50: 313–328.
Harris A.R., Schisler D.A., Neate S.M., Ryder M.H. 1994. Suppression of damping-off caused by Rhizoctonia solani and growth promotion, in bedding plants by binucleate Rhizoctonia spp. Soil Biol. 26: 263–268.
Heydari A., Misaghi I.J. 1997. Effects of three soil applied herbicides on populations of plant disease suppressing bacteria in the cotton rhizosphere. Plant and Soil195: 75–81.
Heydari A., Misaghi I.J. 1998.The impact of herbicides on the incidence and development of Rhizoctonia solani-induced cotton seedling damping-off. Plant Dis.82: 110–113.
Heydari A., Misaghi I.J. 2003.The role of rhizosphere bacteria in herbicide-mediated increase in Rhizoctonia solani-induced cotton seedling damping-off. Plant and Soil 257: 391–396.
Heydari A., Gharedaghli A. 2007.Integrated Pest Management on Cotton in Asia and North Africa.INCANA Press, 103 pp.
Howell C.R., James E., Richard H., William E. 1997.Field control of cotton seedling diseases with Trichoderma virens in combination with fungicide seed treatments.J. Cotton Sci. 1: 15–20.
Howie W., Suslow T. 1986. Effect of antifungal compound biosynthesis on cotton root colonization and Pythium suppression by a strain of Pseudomonas floursences and its antifungal minus isogenic mutant. Phytopathology 70, p. 1069.
Jayaraj J., Radhakrishnan N.V., Kannan R., Sakthivel K., Suganya D., Venkatesan S., Velazhahan R. 2005. Development of new formulations of Bacillus subtilis for management of tomato damping-off caused by Pythium aphanidermatum. Biocontrol Sci. Technol. 15 (1): 55–65.
Kannan R., Jayaraj J. 1998. Effect of various levels of inoculation of Bacillus subtilis on the incidence of damping-off of tomato and on plant growth parameters. Annamalai University Agricul. Res. Ann. 16: 25–30.
Khodakaramian A., Heydari A., Balestra G.M. 2008. Evaluation of Pseudomonads bacterial isolates in biological control of citrus bacterial canker disease. Intern. J. Agricul. Res. 3 (4): 268–272.
Kloepper J.W. 1991. Plant growth-promoting rhizobacteria as biological control agents of soil borne diseases. p.142–152. In: “The Biological Control of Plant Diseases – Progress and Challenges for the Future” (E.C. Tjamos, C.C. Papavizas, R.J. Cook, eds.). NATO ASI Series, 230 pp.
Krebs B., Hoding B., Kubart S., Workie M.A., Junge H., Schmiedeknechr G., Grosch R., Bochow W., Hevesi M. 1998. Use of Bacillus subtilis as biocontrol agent. I. Activities and characterization of Bacillus subtilis strains. J. Plant Dis. Protection 105: 181–197.
Loeffler W., Katzer W., Kremer S., Kugler M., Petersen F., Jung G., Rapp C., Tschen J.S.M. 1990. Gegen pilze wirksame Antibiotika der Bacillus subtilis-GB 03-Gruppe. Forum Microbiol. 3: 156–163.
Maarten P., Smalla K., Berg G. 2000. Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging to Bacillus subtilis. J. Appl. Microbiol. 89: 463–471.
Mansoori B., Hamdollahzadeh A. 1995. Seed rot and seedling diseases of cotton in Gorgan and Gonbad. Appl. Entomol. Phytopathol. 62: 80–83.
Nandakumar R., Viswanathan R., Babu S., Sheela J., Raghuchander T., Samiyappan R. 2001. A new bio-formulation containing plant growth promoting rhizobacterial mixture for the management of sheath blight and enhanced grain yield in rice. Biocontrol 46: 493–510.
Naraghi L., Zareh-Maivan H., Heydari A., Afshari-Azad H. 2007. Investigation of the effect of heating, vesicular arbuscular mycorrhiza and thermophilic fungus on cotton wilt disease. Pak. J. Biol. Sci. 10 (10): 1596–1603.
Podile A.R., Laxmi V.D.V. 1998. Seed bacterization with Bacillus subtilis AF 1 increase phenylalanine ammonia-lyase and reduces the incidence of Fusarium wilt in pigeon pea. J. Phytopathol. 146: 255–259.
Podile A.R., Prakash A.P. 1996. Lysis and biological control of Aspergillus Niger by Bacillus subtilis AF 1. Can. J. Microbiol. 42: 533–538.
Ryder M.H., Zhinong Y., Terrace T.E., Rovira R.D., Wenhua T., Carrell R.L., Yan Z., Tang W. 1999. Use of strains of Bacillus isolated in China to suppress take-all and Rhizoctonia root rot, and promote seedling growth of glasshouse-grown wheat in Australian soils. Soil Biol. Biochem. 31: 19–29.
Schmiedeknecht G., Bochow H., Junge H. 1998. Use of Bacillus subtilis as biocontrol agent. II. Biological control of potato diseases. J. Plant Dis. Protection 105: 41–48.
Sivan A., Chet I. 1986. Biological control of Fusarium sp. in cotton, wheat and muskmelon by Trichoderma harzianum. Phytopathology 116: 39–47.
Spinks D.S., Rowe R.C. 1989. Evaluation of Talaromyces flavus as a biological control agent against Verticillium dahliae in potato. Plant Dis. 73: 230–236.
Sridhar R., Ramakrishnan G., Dinakaran D., Jeyarajan R. 1993. Studies on the efficacy of different carriers for antagonistic Bacillus subtilis. J. Biol. Control 7: 112–114.
Thompson D.C. 1996. Evaluation of bacteria immunization; an alternative to pesticides for control of plant disease in greenhouse and field. p. 30–40. In: “The Biological Control of Plant Disease” (J. Bay-Peterson, ed.). Food and Fertilizer Technology Centre,Tajwan.
Vidhyasekaran P., Muthuamilan M. 1995. Development of formulation of Pseudomonas flourescens for control of chickpea wilt. Plant Dis. 79: 780–782.
Vidhyasekaran P., Rabindran R., Muthamilan M., Nayar K., Rajappan K., Subramanian N., Vasumathi K. 1997. Development of a powder formulation of Pseudomonas flourescens for control of rice blast. Plant Pathol. 46: 291–297.
Zhang J.X., Howell C.R., Starr J.L. 1996. Suppression of Fusarium colonization of cotton roots and Fusariumwilt by seed treatment with Gliocladium virens and Bacillus subtilis. Biocontrol Sci. Technol. 6: 175–187.
Journals System - logo
Scroll to top