Plants can recognize molecules derived from pathogens and trigger systemic acquired resistance (SAR). In phytopathogenic bacteria, elicitors are constituent components of cellular structures, such as flagellin. We sought to select structural components of Xanthomonas spp. incompatible with tomato, aiming to control bacterial spot (Xanthomonas perforans). Initially, cell suspensions from 11 Xanthomonas spp. isolates were infiltrated into the leaves to assess their ability to cause a hypersensitivity response (HR) and the incompatible ones had their flagellin purified. The flagellin of the isolates were first applied at different concentrations, via infiltration and spraying. The pathogen, X. perforans, was inoculated after 24 h, to assess whether there would be any harmful reaction. No harmful reaction was observed in any treatment. Then, a second experiment was conducted to assess the severity of all isolates, at a concentration of 8.35 μg · ml–1, via spraying, infiltration, and soil. The greatest reduction in Area Under the Disease Progress Curve (AUDPC) was observed in the treatment with XapRR, applied via spraying. Thus, prospecting for elicitors is the first step in developing a product for agricultural use. The flagellin elicitor of XapRR is promising and capable of producing these molecules on a large scale.
Camila would like to thank The Universidade Estadual Paulista “Júlio de Mesquita Filho” – Faculdade de Ciências Agronômicas for the opportunity to do her master’s, Embrapa Meio Ambiente for giving space in laboratories and greenhouses to carry out this research and Wirton Macedo Coutinho, Daniel Augusto Schurt, Alessandra Keiko Nakasone, and Nadson de Carvalho Pontes for sending the isolates.
This study was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) – Finance Code 001. Financial support was received from Capes/Programa de Demanda Social (CAPES/DS). Bernardo de Almeida Halfeld- -Vieira thanks the National Council for Technological and Scientific Development (CNPq) for his research productivity fellowship (Proc. 303396/2018-0).
The authors have declared that no conflict of interests exist.
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