Serological characterization of Prune dwarf virus isolates
More details
Hide details
Department of Plant Pathology, Warsaw University of Life Sciences Nowoursynowska 159, 02-776 Warsaw, Poland
Dipartimento di Protezione delle Piante e Microbiologia Applicata Universitả degli Studi di Bari and CNR, Istituto di Virologia Vegetale, Unit of Bari Via Amendola 165/A, 70126 Bari, Italy
Corresponding author
Kinga Sala-Rejczak
Department of Plant Pathology, Warsaw University of Life Sciences Nowoursynowska 159, 02-776 Warsaw, Poland
Journal of Plant Protection Research 2011;51(4):389-392
Prune dwarf virus (PDV), a worldwide pathogen of stone fruit trees, has many isolates with different biological, serological and molecular properties. Monoclonal antibodies (MAbs) to the Prunus mahaleb isolate of PDV were used to investigate the serological variability of virus isolates, by TAS-ELISA (triple antibody sandwich enzyme-linked immunosorbent assay). The twenty-two PDV isolates from Germany (1), Italy (7), Poland (13) and the USA (1) were characterised against eight single MAbs. The virus showed high serological variability. Analysis of the MAbs reaction allowed for the identification of 13 serogroups
The authors have declared that no conflict of interests exist.
Boari A., Boscia D., Yurtmen M., Potere O., Turturo C., Savino V. 1997. Production of monoclonal antibodies to prune dwarf ilarvirus and their use in the serological characterisation of almond virus isolate. OEPP/EPPO Bull. 27: 555–556.
Clark M.F., Adams A.N. 1977. Characteristic of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34: 475–483.
Fauquet C.M., Mayo M.A., Maniloff J., Desselberger U., Ball L.A. 2005. Virus Taxonomy. Classifiction and Nomenclature of Viruses. p. 1049–1059. In: Eight Report of the International Committee on the Taxonomy of Viruses. Virology Division International Union of Microbiological Societies, Elsevier Academic Press Amsterdam, Boston, Heidelberg, London, New York, Oxford, Paris, San Diego, San Francisco, Singapore, Sydney, Tokyo, 1162 pp.
Fonseca F., Neto J.D., Martins V., Nolasco G. 2005. Genomic variability of Prune dwarf virusas affected by agricultural practice. Arch. Virol. 150: 1607–1619.
Fulton R.W. 1970. Prune dwarf virus. C.M.I./A.A.B. Description of Plant Viruses. No.19.Jordan R., Matsumoto T., Hei-Ti H. 1991. Evaluation and application of prune dwarf virus-specific monoclonal antibodies in virus detection and disease diagnosis. Phytopathology 81, p. 1217. (Abstract).
Paduch-Cichal E. 2000. Wielostronna charakterystyka izolatów wirusa nekrotycznej pierścieniowej plamistości wiśni i wirusakarłowatości śliwy. [The Characterization of PNRSV and PDV Isolates]. Fundation: ‘Rozwój SGGW’, Warsaw, 104 pp. (in Polish).
Speigiel S., Scott S.W., Bowman-Vance V., Tam Y., Galiakparov N.N., Rosner S.A. 1996. Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction. Eur. Biol. 122: 493–500.
Ulubaş Serçe Ç., Ertunç F., Öztrük A. 2009. Identification and genomic variability of Prune dwarf virus variants infecting stone fruit trees in Turkey. J. Phytopathol. 157: 298–305.
Waterworth H.E., Fulton R.W. 1964. Variation among isolates of necrotic ringspot and Prune dwarf viruses isolated from sour cherry. Phytopathology 54: 1155–1160.
Vaškova D, Pertzik K., Špak J. 2000. Molecular variability of the capsid protein of the Prune dwarf virus. Eur. J. Plant Pathol. 106: 573–580.
Journals System - logo
Scroll to top