Serological characterization of Prune dwarf virus isolates
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Department of Plant Pathology, Warsaw University of Life Sciences Nowoursynowska 159, 02-776 Warsaw, Poland
Dipartimento di Protezione delle Piante e Microbiologia Applicata Universitả degli Studi di Bari and CNR, Istituto di Virologia Vegetale, Unit of Bari Via Amendola 165/A, 70126 Bari, Italy
Corresponding author
Kinga Sala-Rejczak
Department of Plant Pathology, Warsaw University of Life Sciences Nowoursynowska 159, 02-776 Warsaw, Poland
Journal of Plant Protection Research 2011;51(4):389–392
Prune dwarf virus (PDV), a worldwide pathogen of stone fruit trees, has many isolates with different biological, serological and molecular properties. Monoclonal antibodies (MAbs) to the Prunus mahaleb isolate of PDV were used to investigate the serological variability of virus isolates, by TAS-ELISA (triple antibody sandwich enzyme-linked immunosorbent assay). The twenty-two PDV isolates from Germany (1), Italy (7), Poland (13) and the USA (1) were characterised against eight single MAbs. The virus showed high serological variability. Analysis of the MAbs reaction allowed for the identification of 13 serogroups
The authors have declared that no conflict of interests exist.
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