Signaling molecules from Lactuca sativa L. induced quorum sensing phenotypes in bacteria
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Department of Plant Protection, Islamic Azad University, Isfahan (Khorasgan) Branch, P.O. Box 81595-158 Isfahan, Iran
Submission date: 2014-08-30
Acceptance date: 2015-04-13
Corresponding author
Esmaeil Mahmoudi
Department of Plant Protection, Islamic Azad University, Isfahan (Khorasgan) Branch, P.O. Box 81595-158 Isfahan, Iran
Journal of Plant Protection Research 2015;55(2):166-171
The outcome of the present investigation revealed that lettuce (Lactuca sativa L.) extract strongly interferes with acyl homoserine lactone (AHL) regulated physiological functions in the bioreporter strain, Chromobacterium violaceum CV026. Extracts of L. sativa also promoted production of virulence factors in plant pathogen, Pectobacterium carotovorum, and significantly increased tissue maceration on potato tubers when there was a low concentration (10 3 cfu · ml–1) of the bacterium was used. The thin layer chro- matogram which visualised with AHL bioreporter strain, CV026, showed that L. sativa extract produced a circular spot with a diffuse edge tail and migrated with the same mobility as standard N-hexanoyl-L-homoserine lactone (C6-HSL). Gas Chromatography Mass Spectroscopy (GC-MS) analysis of the lettuce extract resulted in the identification of 19 compounds of which homoserine was identified for the first time in plants. Homoserine accounted for 2.37% of the total constituents. It is a new finding that lettuce contains AHL like substances (homoserine) which excite AHL related quorum sensing (QS) in bacteria.
The authors have declared that no conflict of interests exist.
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