ORIGINAL ARTICLE
The toxicity of Cry1Ia5 transgenic soybean plants against Spodoptera littoralis
| 1 | Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt |
| 2 | Department of Field Crop Pests, Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Etr Hussein Khashaba
Department of Field Crop Pests, Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt
Department of Field Crop Pests, Plant Protection Research Institute, Agriculture Research Center, Dokki, Egypt
Submission date: 2019-02-10
Acceptance date: 2019-05-31
Online publication date: 2019-06-27
Journal of Plant Protection Research 2019;59(2):185–191
KEYWORDS
TOPICS
ABSTRACT
Three transgenic soybean lines expressing the Cry1Ia5 gene were developed using the Agrobacterium
transformation system. The integration of the Cry1Ia5 gene in the genome of the
transgenic plants was approved using specific primers for PCR and real time PCR analysis,
respectively. The insecticidal activity of three transgenic lines (L1, L2 and L3) against 2nd
larval instars Spodoptera littoralis was tested. The data indicate that L2 exhibited the highest
mortality percentage 9 days post feeding (60%) followed by L3 (40%) then L1 (20%) while
the control showed 0% mortality. The larvae fed transgenic material appeared smaller in
size than compared to the control larvae. The reduction in insect size and weight was due
to the accumulation of higher phenoloxidase activity in insect tissues. The higher mortality
observed in L2 was due to a significant decrease in the acetylcholine esterase activity that
leads to accumulation of acetylcholin at higher levels which causes paralysis and death. The
developed transgenic line 2 could be used to construct an insect resistant soybean cultivar.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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