ORIGINAL ARTICLE
‘Bois noir': new phytoplasma disease of grapevine in Iran
 
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Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
CORRESPONDING AUTHOR
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Submission date: 2014-10-30
Acceptance date: 2015-02-17
 
Journal of Plant Protection Research 2015;55(1):88–93
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ABSTRACT
Recently, grapevines showing symptoms suggesting the ‘bois noir’ phytoplasma disease were observed in vineyards located in several central provinces of Iran. Polymerase chain reaction assays using phytoplasma universal primer pair P1A/P7A followed by primer pair R16F2n/R16R2 in nested PCR, confirmed the association of phytoplasmas with symptomatic grapevines. The results of RFLP analyses using Hpa II, Hinf I, Mse I, Rsa I, and Taq I restriction enzymes, indicated that grapevine phytoplasma isolates in these regions could be related to the 16SrXII group. Sequence analyses of the partial 16S rRNA gene confirmed that Iranian grapevine phytoplasmas are associated with ‘Candidatus Phytoplasma solani’. This is the first report of the ‘bois noir’ disease outbreak in Iran.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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