ORIGINAL ARTICLE
Biochemical characterisation of the tissue degrading enzyme, collagenase, in the spined soldier bug, Podisus maculiventris (Hemiptera: Pentatomidae)
 
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Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
CORRESPONDING AUTHOR
Mahboob Ghamari
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
Submission date: 2013-01-06
Acceptance date: 2014-05-20
 
Journal of Plant Protection Research 2014;54(2):164–170
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ABSTRACT
Podisus maculiventris (Say) is a generalist predator attacking many insect species from different orders. The bug injects saliva into its prey’s body. The ingested hemolymph and liquefied internal tissues pass through the bug’s alimentary tract. Collagenase working on peptide bonds of collagen and basement membrane proteins, leads to the disintegration of the prey’s internal organs. As yet, there is an almost complete lack of knowledge on the collagenase activity in P. maculiventris. The collagenase activity of the salivary glands and midgut was optimum at pH 8.0 which was congruent with the optimal pH of the total proteolytic activity of the salivary glands. More collagenolytic activity was determined in the posterior lobe of the salivary glands and anterior midgut. Significant inhibition of collagenolytic activity by ethylenediaminetetraacetic acid (EDTA) revealed the enzyme is a metalloproteinase. The collagenase activity notably decreased when the bug went hungry. The salivary gland collagenase is a vital enzyme in extra-oral digestion and facilitates the action of other digestive enzymes. The midgut collagenase may be involved in the digestion of the ingested muscle fibers. The collagenase probably acts as an intoxicating agent in the saliva (venom) of P. maculiventris. Paralysing toxins are present in the salivary gland secretion.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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