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Detection of Russian olive witches’-broom disease and its insect vector in Northwestern Iran
 
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Department of Plant Protection, Faculty of Agriculture, University of Tabriz, 5166616471, Tabriz, Iran
CORRESPONDING AUTHOR
Reza Khakvar
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, 5166616471, Tabriz, Iran
Submission date: 2017-01-03
Acceptance date: 2017-07-12
 
Journal of Plant Protection Research 2017;57(3):309–313
 
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ABSTRACT
Recently, Russian olive trees showing witches’-broom and little leaf symptoms have been widely observed in northwestern and central Iran. Polymerase chain reaction (PCR) and nested PCR assays using phytoplasma universal primer pairs confirmed phytoplasma symptomatic infection of trees. Sequence analyses showed that ‘Candidatus Phytoplasma asteris’ was the causal agent of the disease in these regions. However, RFLP results using restriction enzymes HpaII, EcoRI, HinfI and AluI indicated that the collected isolates in these regions are genetically different. In addition, leafhopper Macropsis infuscata was recognized as a possible insect vector of the disease for the first time.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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