Characterization of phytoplasmas related to ‘Candidatus Phytoplasma asteris' subgroup rpI-L in Iran
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Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Submission date: 2013-12-14
Acceptance date: 2014-05-30
Corresponding author
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Journal of Plant Protection Research 2014;54(2):199-203
In two of Iran’s central provinces, several herbaceous plants showing phytoplasma disease symptoms were collected to detect ‘Canididatus Phytoplasma asteris’-related phytoplasmas. Confirmation of an association of phytoplasmas with diseased plants was done using polymerase chain reaction (PCR) assays having the phytoplasma universal primer pairs P1/P7 followed by R16F2n/R16R2 in nested PCR. Then, for detection of ‘Ca. P. asteris’, DNA samples were subjected to amplification of rp and tuf genes using specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment length polymorphism or RFLP analyses of rp gene fragments using Tsp 509I restriction enzyme as well as sequence analyses indicated that ‘Ca. P. asteris’-related phytoplasmas associated with carrot, niger seed and scallion plants in these regions, belong to the rp I-L subgroup. This research is the first report of carrot, niger seed, and scallion infection with phytoplasmas belonging to the rp I-L subgroup.
The authors have declared that no conflict of interests exist.
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