‘Bois noir': new phytoplasma disease of grapevine in Iran
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Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Submission date: 2014-10-30
Acceptance date: 2015-02-17
Corresponding author
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran
Journal of Plant Protection Research 2015;55(1):88-93
Recently, grapevines showing symptoms suggesting the ‘bois noir’ phytoplasma disease were observed in vineyards located in several central provinces of Iran. Polymerase chain reaction assays using phytoplasma universal primer pair P1A/P7A followed by primer pair R16F2n/R16R2 in nested PCR, confirmed the association of phytoplasmas with symptomatic grapevines. The results of RFLP analyses using Hpa II, Hinf I, Mse I, Rsa I, and Taq I restriction enzymes, indicated that grapevine phytoplasma isolates in these regions could be related to the 16SrXII group. Sequence analyses of the partial 16S rRNA gene confirmed that Iranian grapevine phytoplasmas are associated with ‘Candidatus Phytoplasma solani’. This is the first report of the ‘bois noir’ disease outbreak in Iran.
The authors have declared that no conflict of interests exist.
Boudon-Padieu E. 2003. The situation of grapevine yellows and current research directions: distribution, diversity, vectors, diffusion and control. p: 47–53. In: Extended abstracts from the 14th meeting of the ICVG (International Council for the Study of Virus and Virus-like Diseases of the Grapevine), Locorotondo (Bari), Italy, 12–17 September 2003, 256 pp.
Canik D., Ertunc F., Paltrinieri S., Contaldo N., Bertaccini A. 2011. Identification of different phytoplasmas infecting grapevine in Turkey. Bulletin Insectology 64 (Supplement): 225–226.
Caudwell A. 1961. A study of black wood disease of vines: its relationship to flavescence dorée. Annales des Epiphyties 12 (3): 241–262.
Choueiri E., Jreijiri F., El Zammar S. 2002. First report of grapevine “Bois Noir” disease and a new phytoplasma infecting solanaceous plants in Lebanon. Plant Disease 86 (6): 697.
Davis R.E., Dally E.L., Gundersen D.E., Lee I.M., Habili N. 1997. “Candidatus Phytoplasma australiense”, a new phytoplasma taxon associated with Australian grapevine yellows. International Journal of Systematic and Evolutionary Microbiology 47 (2): 262–269.
Gajardo A., Fiore N., Prodan S., Paltrinieri S., Botti S., Pino A.M., Zamorano A., Montealegre J., Bertaccini A. 2009. Phytoplasmas associated with grapevine yellows disease in Chile. Plant Disease 93 (8): 789–796.
Hosseini P., Bahar M., Madani G., Zirak L. 2011. Molecular characterization of phytoplasmas associated with potato purple top disease in Iran. Journal of Phytopathology 159 (4): 241–246.
Langer M., Maixner M. 2004. Molecular characterization of grapevine yellows associated phytoplasmas of the stolburgroup based on RFLPanalysis of nonribosomal DNA. Vitis 43 (4): 191–200.
Lashkari M., Nouri Ganbalani G., Mozaffarian F., Ghorbani K.H., Fathi A. 2009. Faunistic study of planthoppers infraorder Fulgoromorpha (Hem., Auchenorrhyncha) in different climatic regions of Gorgan. Journal of Entomological Research 2 (2): 119–133.
Lee I.M., Martini M., Marcone C., Zhu S.F. 2004. Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus Phytoplasma ulmi’ for the phytoplasma associated with elm yellows. International Journal of Systematic and Evolutionary Microbiology 54 (2): 337–347.
Lee I.M., Hammond R.W., Davis R.E., Gundersen D.E. 1993. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83 (8): 834–842.
Marzorati M., Alma A., Sacchi L., Pajoro M., Palermo S., Brusetti L., Raddadi N., Balloi A., Tedeschi R., Clementi E., Corona S., Quaglino F., Attilio Bianco P., Beninati T., Bandi C., Daffonchio D. 2006. A novel bacteroidetes symbiont is localized in Scaphoideus titanus, the insect vector of Flavescence Dorée in Vitis vinifera. Applied of Environmental Microbiology 72 (2): 1467–1475.
Murray M.G., Thompson W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Results 8 (19): 4321–4325.
Prince J.P., Davis R.E., Wolf T.K., Lee I.M., Mogen B.D., Dally E.L., Bertaccini A., Credi R., Barba M. 1993. Molecular detection of diverse mycoplasmlike organisms (MLOs) associated with grapevine yellows and their classification with aster yellow, X-disease, and elm yellows phytoplasmas. Phytopathology 83 (10): 1130–1137.
Quaglino F., Zhao Y., Casati P., Bulgari D., Bianco P.A., Wei W., Davis R.E. 2013. ‘Candidatus Phytoplasma solani’, a novel taxon associated with stolbur- and Bois noir-related diseases of plants. International Journal of Systematic and Evolutionary Microbiology 63 (8): 2879–2894.
Salehi M., Heydarnejad J., Izadpanah K. 2005. Molecular characterization and grouping of 35 phytoplasmas from central and southern provinces in Iran. Iranian Journal of Plant Pathology 41 (1): 62–65.
Salem N.M., Quaglino F., Abdeen A., Casati P., Bulgari D., Alma A., Bianco P.A. 2013. First report of ‘Candidatus Phytoplasma solani’ associated with grapevine Bois noir disease in Jordan. p. 23–24. COST Action FAO 807 Final Meeting. Integrated Management of Phytoplasma Epidemics in Different Crop Systems. Lisbon, Portugal, September 30–October 1, 85 pp.
Schvester D., Carle P., Moutous G. 1961. Sur la transmission de la flavescence dorée des vignes par une cicadelle. [The transmission of grapevine phytoplasma in vineyards by leafhopper]. Comptes Rendus de Séances de l’Académie d’Agriculture de France 47: 1021–1024.
Vali Sichani F., Bahar M., Zirak L. 2011. Characterization of stolbur (16SrXII) group phytoplasmas associated with Cannabis sativa witches’-broom disease in Iran. Plant Pathology Journal 10 (4): 161–167.
Weintraub P.G., Jones P. 2010. Phytoplasmas Genomes, Plant Hosts and Vectors. Wallingford, UK, CABI Publishing, 352 pp.
Zirak L., Bahar M. , Ahoonmanesh A. 2009a. Characterization of phytoplasmas associated with almond diseases in Iran. Journal of Phytopathology 157 (11–12): 736–741.
Zirak L., Bahar M., Ahoonmanesh A. 2009b. Molecular characterization of phytoplasmas related to peanut witches-broom and stolbur groups infecting plum in Iran. Journal of Plant Pathology 91 (3): 713–716.
Zirak L., Bahar M., Ahoonmanesh A. 2010. Molecular characterization of phytoplasmas associated with peach diseases in Iran. Journal of Phytopathology 158 (2): 105–110.
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