ORIGINAL ARTICLE
Effect of Drechslera graminea on total soluble proteins and defense enzymes of barley
 
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Amity Institute of Biotechnology, Amity University, Plot No. 48a, Knowledge Park III, Greater Noida, Uttar Pradesh, India
Online publish date: 2018-09-26
Submission date: 2018-02-05
Acceptance date: 2018-04-11
 
Journal of Plant Protection Research 2018;58(3)
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ABSTRACT:
Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf stripe disease in the hos. The present study involved the elucidation of alterations induced in the protein content of the host due to Drechslera infection. Naturally growing barley plants were obtained from fields and Drechslera graminea was isolated and identified from diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen was inoculated on plants grown under aseptic and controlled laboratory conditions. Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated a significant effect of the pathogen on the cytoplasmic protein expression of the host as well as in its defense system.
CORRESPONDING AUTHOR:
Navodit Goel   
Amity Institute of Biotechnology, Amity University, Plot No. 48a, Knowledge Park III, Greater Noida, Uttar Pradesh, India
 
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