ORIGINAL ARTICLE
Effectiveness of the chemical stabilizers of Talaromyces flavus in biological control of tomato and greenhouse cucumber vascular wilt disease
 
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1
Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran
2
Plant Disease Research Department, Iranian Research Institute of Plant Protection, P.O. Box 1452, Tehran 19395, Iran
Submission date: 2016-04-29
Acceptance date: 2016-08-22
 
Journal of Plant Protection Research 2016;56(3):291–297
KEYWORDS:
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ABSTRACT:
Fungal antagonist, Talaromyces flavus, is one of the most important biological agents of soil-borne fungal diseases including Verticillium and Fusarium wilt. In this study, to increase the effectiveness of T. flavus isolates obtained from greenhouse cucumbers and field grown tomatoes five chemical stabilizers were evaluated. Based on the results of previous studies, the most effective substrate for the growth, sporulation and stability of T. flavus isolates related to the above-mentioned plants was a mix of rice bran and peat- moss. Different chemical stabilizers were mixed with the above-mentioned substrate containing spore suspensions of various T. flavus isolates. For each plant, a completely randomized experiment was conducted under greenhouse conditions with seven treatments and three replications. The results of this study indicated that treatments containing sodium nitrate and D-cycloserine were more effective than those containing other stabilizers. The overall results of this study suggest that the use of some chemical stabilizers may enhance the biocontrol potential of fungal antagonists in controlling different plant diseases including Verticillium and Fusarium wilt.
CONFLICT OF INTERESTS:
The authors have declared that no conflict of interests exist.
CORRESPONDING AUTHOR:
Laleh Naraghi
Plant Disease Research Department, Iranian Research Institute of Plant Protection, P.O. Box 1452, Tehran 19395, Iran
 
REFERENCES (47):
1. Alimi T., Ajewole O.C., Olubode-Awosola O.O., Idowu E.O. 2006. Economic rationale of commercial organic fertilizer technology in vegetable production in Osun State of Nigeria. Journal of Applied Horticulture 8 (2): 159–164.
2. Anitha A., Rabeeth M. 2010. Degradation of fungal cell walls of phytopathogenic fungi by lytic enzyme of Streptomyces griseus. African Journal of Plant Science 4 (3): 61–66.
3. Ashraf M.S., Khan T.A. 2007. Efficacy of Gliocladium virens and Talaromyces flavus with and without organic amendments against Meloidogyne javanica infecting eggplant. Asian Journal of Plant Pathology 1 (1): 18–21.
4. Ausher R., Katan J., Ovadia S. 1975. An improved selective medium for the isolation of Verticillium dahliae. Phytoparasitica 3 (2): 133–137.
5. Aziz N.H., El-Fouly M.Z., El- Essawy A.A., Khalaf M.A. 1997. Influence of bean seedling root exudates on the rhizosphere colonization by Trichoderma lignorum for the control of Rhizoctonia solani. Botanical Bulletin of Academia Sinica 38 (1): 33–39.
6. Budge S.P., Whipps J.M. 2001. Potential for integrated control of Sclerotinia sclerotiorum in glasshouse lettuce using Coniothyrium minitans and reduced fungicide application. Phytopathology 91 (2): 221–227.
7. Christen A.A. 1982. A selective medium for isolating Verticillium albo-atrum from soil. Phytopathology 72 (1): 47–49.
8. Cimarelli C., Palmieri G., Volpini E. 2001. Ready N-alkylation of enantiopure aminophenols: synthesis of tertiary aminophenols. Tetrahedron 57 (28): 6089–6096.
9. Damaso M.C.T., Terzi S.C., Farias A.X., de Oliveira A.C.P., Fraga M.E., Couri S. 2012. Selection of cellulolytic fungi isolated from diverse substrates. Brazilian Archives of Biology and Technology 55 (4): 513–520.
10. Duo-Chuan L.I., Chen S., Jing L.U. 2005. Purification and partial characterization of two chitinases from the mycoparasitic fungus Talaromyces flavus. Mycopathologia 159 (2): 223–229.
11. Gavanji S., Aziz H.A., Larki B., Mojiri A. 2013. Computational prediction and analysis of interaction of silver nitrate with chitinase enzyme. International Journal of Scientific Research in Environmental Sciences 1 (4): 50–62.
12. Ghaderi F. 2011. The role of Pythium aphanidermatum and Phytophthora melonis in root and crown rot on greenhouse cucumber in Yasouj. Iranian Journal of Plant Pathology 47 (3): 101–111.
13. Ghaderi-Daneshmand N., Bakhshandeh A., Rostami M.R. 2012. Biofertilizer affects yield and yield components of wheat. International Journal of Agriculture Research and Review 2 (6): 699–704.
14. Hadar E., Katan J. 1989. The use of nitrate non-utilizing mutants and a selective medium for studies of pathogenic strains of Fusarium oxysporum. Plant Disease 73 (10): 800–803.
15. Haggag W.M., Kansoh A.L., Aly A.M. 2006. Proteases from Talaromyces flavus and Trichoderma harzianum: purification, characterization and antifungal activity against brown spot disease on faba bean. Plant Pathology Bulletin 15 (4): 231–239.
16. Hao Z., Christie P., Qin L., Wang C., Li X. 2005. Control of Fusarium wilt of cucumber seedling by inoculation with an arbuscular mycorrhical fungus. Journal of Plant Nutrition 28 (11): 1961–1974.
17. Hiscox J., Savoury M., Vaughan I.P., Müller C.T., Boddy L. 2015. Antagonistic fungi interactions influence carbon dioxide evolution from decomposing wood. Fungal Ecology 14 (1): 24–32.
18. Husen E., Simanungkalit R.D.M., Suraswati R., Irawan I. 2007. Characterization and quality assessment of Indonesian commercial biofertilizers. Indonesian Journal of Agricultural Science 8 (1): 31–38.
19. Inbar J., Chet I. 1995. The role of recognition in the induction of specific chitinases during mycoparasitism by Trichoderma harzianum. Microbiology 141 (11): 2823–2829.
20. Jat J.G., Agalave H.R. 2013. Antagonistic properties of Trichoderma species against oilseed-borne fungi. Science Research Reporter 3 (2): 171–174.
21. Kaewchai S., Soytong K., Hyde K.D. 2009. Mycofungicides and fungal biofertilizers. Fungal Diversity 38 (1): 25–50.
22. Khalil M.S., Abdel-Sattar M.A., Aly I.N., Abd-Elsalam K.A., Verreet J.A. 2003. Genetic affinities of Fusarium spp. and their correlation with origin and pathogenicity. African Journal of Biotechnology 2 (5): 109–113.
23. Kim K.K., Fravel D.R., Papavizas G.C. 1989. Identification of a metabolite produced by Talaromyces flavus as glucose oxidase and its role in the biocontrol of Verticillium dahliae. Phytopathology 78 (4): 488–492.
24. Koch E. 1999. Evaluation of commercial products for microbial control of soil-borne plant disease. Crop Protection 18 (2): 119–125.
25. Lee S., Lee J.W. 2009. Color stabilization of low toxic antimicrobial polypropylene/poly (hexamethylene guanidine) phosphate blends by Taguchi technique. Macromolecular Research 17 (6): 411–416.
26. Madi L., Katan T., Henis Y. 1992. Inheritance of antagonistic properties and lytic enzyme activities in sexual crosses of Talaromyces flavus. Annals of Applied Biology 121 (3): 565–576.
27. Madi L., Katan T., Katan J., Henis Y. 1997. Biological control of Sclerotium rolfsii and Verticillium dahliae by Talaromyces flavus is mediated by different mechanisms. Phytopathology 87 (10): 1051–1060.
28. Matos M., Simpson B.K., Ramírez H.L., Cao R., Torres-Labandeira J.J., Hernández K. 2012. Stabilization of glucose oxidase with cyclodextrin-branched carboxymethylcellulose. Biotechnología Aplicada 29 (1): 29–34.
29. Menz G., Aldred P., Vriesekoop F. 2011. Growth and survival of food borne pathogens in beer. Journal of Food Protection 74 (10): 1670–1675.
30. Mukhopadhyay S., Maiti S.K. 2009. Biofertilizer: VAM fungi – A future prospect for biological reclamation of mine degraded lands. Indian Journal of Environmental Protection 29 (9): 801–808.
31. Naraghi L., Heydari A., Karimi Roozbahani A., Ershad D. 2003. Isolation of Talaromyces flavus from cotton fields in Gorgan and its antagonistic effects on Verticillium dahliae, the causal agent of cotton wilt. Iranian Journal of Plant Pathology 39 (3–4): 109–122. (in Persian, with English summary).
32. Naraghi L., Heydari A., Rezaee S., Razavi M. 2012. Biocontrol agent Talaromyces flavus stimulates the growth of cotton and potato. Journal of Plant Growth Regulation 31 (4): 471–477.
33. Naraghi L., Heydari A., Rezaee S., Razavi M. 2013. Study on some antagonistic mechanisms of Talaromyces flavus against Verticillium dahliae and Verticillium albo-atrum, the causal agents of wilt disease in several important crops. Biocontrol in Plant Protection 1 (1): 13–28. (in Persian, with English summary).
34. Naraghi L., Heydari A., Rezaee S., Razavi M., Jahanifar H., Mahmoodi Khaledi E. 2010. Biological control of tomato Verticillium wilt disease by Talaromyces flavus. Journal of Plant Protection Research 50 (3): 360–365.
35. Pascual S., Melgarejo P., Magan N. 1999. Production of the fungal biocontrol agent Epicoccum nigrum by solid substrate fermentation: effect of water activity on accumulation of compatible solutes. Mycopathologia 146 (2): 83–89.
36. Patil N.S., Jadhav J.P. 2015. Penicillium ochrochloron MTCC 517 chitinase: An effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. Saudi Journal of Biological Sciences 22 (2): 232–236.
37. Pereira I., Ortega R., Barrientos L., Moya M., Reyes G., Kramm V. 2009. Development of a biofertilizer based on filamentous nitrogen – fixing cyanobacteria for rice crops in Chile. Journal of Applied Phycology 21 (1): 135–144.
38. Sargin S., Gezgin Y., Eltem R., Vardar F. 2013. Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology 37 (2): 139–146.
39. Schuster A., Schmoll M. 2010. Biology and biotechnology of Trichoderma. Applied Microbiology and Biotechnology 87 (3): 787–799.
40. Shanmugam V., Ronen M., Shalaby S., Larkov O., Rachamim Y., Hadar R., Rose M.S., Carmeli S., Horwitz B.A., Lev S. 2010. The fungal pathogen Cochliobolus heterostrophus responds to maize phenolics: novel small molecule signals in a plant-fungal interaction. Cellular Microbiology 12 (10): 1421–1431.
41. Sharzehei A., Heidary S., Raufi F. 2011. Identification of tomato root and crown pathogenic fungi in Marvdasht region, Iran. Quarterly Journal of Research in Plant Pathology 1 (1): 57–65. (in Persian, with English summary).
42. Vasane S.R., Kothari R.M. 2008. An integrated approach to primary and secondary hardening of banana var. Grand Naine. Indian Journal of Biotechnology 7 (2): 240–245.
43. Veverka K., Stolcova J., Ruzek P. 2007. Sensitivity of fungi to urea, ammonium nitrate and their equimolar solution UAN. Plant Protection Science 43 (4): 157–164.
44. Vincelli P.C., Beaupré C.M-S. 1989. Comparison of media for isolating Rhizoctonia solani from soil. Plant Disease 73 (12): 1014–1017.
45. Wei C.M., Hansen B.S., Vaughan M.H., McLaughlin C.S. 1974. Mechanism of action of the mycotoxin trichodermin, a 12,13-epoxytrichothecene. Proceedings of the National Academy of Sciences of the United States of America 71 (3): 713–717.
46. Yakutkin V.I. 1972. Comparative pathogenicity of two forms of Verticillium dahliae on cotton varieties different by resistance to wilt. Mycology and Phytopathology. Leningrad: Nauka 6 (3): 291–292. (in Russian).
47. Yu M.Y., Chang S.T. 1987. Effects of osmotic stabilizers on the activities of mycolytic enzymes used in fungal protoplast liberation. World Journal of Microbiology and Biotechnology 3 (2): 161–167.
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