Effects of Artemisia annua methanolic extract on the enzymatic components of intermediary metabolism and the antioxidant system of Pseudococcus viburni Signoret
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Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Plant Protection Department, Research Center for Agriculture and Natural Resources, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
Online publish date: 2018-10-18
Submission date: 2018-01-09
Acceptance date: 2018-04-23
Journal of Plant Protection Research 2018;58(3)
Toxicity and physiological alterations were determined in Pseudococcus viburni nymphs treated with Artemisia annua methanolic extract. The leaf dipping bioassay showed LC50 values of 0.287% and 0.194% 24 and 48 hours post-exposure. Activities of general esterases were significantly higher in the control nymphs than in those which had been treated except for the 48 h time interval using α-naphtyl acetate. The activity of glutathione S-transferase using CDNB (1-chloro-2,4-dinitrobenzene) in the control nymphs, was significantly higher than in the control at both time intervals while no significant difference was observed after 24 h in addition to the higher enzymatic activity in the treated nymphs after 48 h. All three aminotransferases were significantly more active in the control nymphs except for time intervals of 24 h for γ-glutamyl transferase and 48 h for alanine aminotransferase. Higher activities of lactate dehydrogenase, acid- and alkaline phosphatase were found in the control nymphs than in treated nymphs for all time intervals. Activities of the enzymes involved in the antioxidant system including catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase was increased in the treated nymphs compared to the control. Results of the current study demonstrated toxic effects of A. annua methanolic extract on P. viburni nymphs causing mortality and physiological turbulences.
Samar Ramzi   
Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Sheikh Zahed Street, 41362536521 Lahijan, Iran
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