ORIGINAL ARTICLE
Mutation at Codon 198 of Tub2 gene for carbendazim resistance in Colletotrichum gloeosporioides causing mango anthracnose in Thailand
1,
1,
2,
2,
2
| 1 | Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University
Chiang Mai 50200 and Center of Excellence on Agriculture Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand |
| 2 | Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan |
CORRESPONDING AUTHOR
Pornprapa Kongtragoul
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University Chiang Mai 50200 and Center of Excellence on Agriculture Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University Chiang Mai 50200 and Center of Excellence on Agriculture Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand
Journal of Plant Protection Research 2011;51(4):377–384
KEYWORDS
TOPICS
ABSTRACT
Screening of field isolates of
Colletotrichum gloeosporioides
from various mango cultivars from markets and orchards in
Thailand identified 113 carbendazim-resistant isolates. Isolates with a highly-resistant phenotype (HR) grew well on Potato Dextrose Agar (PDA) amended with carbendazim even at ≥500 mg/l. Isolates with carbendazim-resistant phenotype had a conspicuous
mutation at a particular site in the β-tubulin (
TUB2
) gene sequence. The sequence of
TUB2
in HR isolates showed a single nucleotide
transversion of adenine to cytosine, resulting in a substitution at codon 198 from glutamic acid (GAG) in wild type to alanine (G
CG) in
HR isolates. This is the first report of the molecular determination of field isolates for benzimidazole fungicide resistance in Southeast
Asia in
C. gloeosporioides
causing mango anthracnose.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (48)
1.
Akem C.N. 2006. Mango anthracnose disease: present status and future research priorities. Plant Pathol. J. 5 (3): 266–273.
2.
Albertini C., Gredt M., Leroux P. 1999. Mutations of the β-tubulin gene associated with different phenotypes of benzimidazole resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis. Pestic. Biochem. Physiol. 64 (1): 17–31.
3.
Baraldi E., Mari M., Chierici E., Pondrelli M., Bertolini P., Pratella G.C. 2003. Studies on thiabendazole resistance of Penicillium expansumof pears: pathogenic fitness and genetic characterization. Plant Pathol. 52 (3): 362–370.
4.
Brent K.J., Hollomon D.W. 1998. Fungicide Resistance: the Assessment of Risk. FRAC Monograph No 2, Brussels, 48 pp.
5.
Buhr T.L., Dickman M.B. 1994. Isolation, characterization, and expression of a second β-tubulin-encoding gene from Colletotrichum gloeosporioides f. sp. aeschynomene. Appl. Environ. Microbiol. 60 (11): 4155–4159.
6.
Cańas-Gutiérrez G.P., Patińo L.F., Rodriguez-Arango E., Arango R. 2006. Molecular characterization of bennomyl-resistant isolates of Mycosphaerella fijiensis, collected in Colombia. J. Phytopathol.154 (7–8): 403–409.
7.
Chung W.-H., Ishii H., Nishimura K., Fukaya M., Yano K., Kajitani Y. 2006. Fungicide sensitivity and phylogenetic relationship of anthracnose fungi isolated from various fruit crops in Japan. Plant Dis. 90 (4): 506–512.
8.
Davides L.C. 1986. Benzimidazole fungicides: mechanism of action and biological impact. Annu. Rev. Phytopathol. 24:43–65.
9.
Davidson R.M., Hanson L.E., Franc G.D., Panella L. 2006. Analysis of β-tubulin gene fragments from benzimidazole-sensitive and -tolerant Cercospora beticola. J. Phytopathol. 154 (7–8): 321–328.
10.
Deising H.B., Reimann S., Pascholati S.F. 2008. Mechanisms and significance of fungicide resistance. Brazil. J. Microbiol. 39 (2): 286–295.
11.
Duamkhanmanee R. 2008. Natural essential oils from lemon grass (Cymbopogon citratus) to control postharvest anthracnose of mango fruit. Int. J. Biotechnol. 10 (1): 104–108.
12.
Farungsang U., Farungsang N. 1992. Benomyl resistance of Colletotrichum spp. associated with rambutan and mango fruit rot in Thailand. Acta Hortic. 321 (2): 891–897.
13.
Farungsang U., Farungsang N., Sangchote S. 1994. Benomyl resistance of Colletotrichum species associated with mango and rambutan fruit rots in Thailand. p. 45–50. In: Proc. an Int. Conference Held in Bangkok, Thailand: Development of Postharvest Handling Technology for Tropical Tree Fruits, 16–18 July 1994, 86 pp.
14.
Freeman S., Minz D., Jurkevitch E., Maymon M., Shabi E.2000. Molecular analyses of Colletotrichum species from almond and other fruits. Phytopathology 90 (6):608–614.
15.
Fujimura M., Oeda K., Inoue H., Kato T. 1992. A single aminoacid substitution in the beta-tubulin gene of Neurosporaconfers both carbendazim resistance and diethofencarb sensitivity. Curr. Genet. 21 (4–5): 399–404.
16.
Gafur A., Tanaka C., Shimizu K., Ouchi S., Tsuda M. 1998. Molecular analysis and characterization of the Cochliobolus heterostrophus β-tubulin gene and its possible role in conferring resistance to benomyl. J. Gen. Appl. Microbiol. 44 (3): 217–223.
17.
Ishii H. 2006. Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment. Jpn. Agric. Res. Q. 40 (3): 205–211.
18.
Kim Y.-S., Min J.Y., Kang B.K., Bach N.V., Choi W.B., Park E.W., Kim H.T. 2007. Analyses of the less benzimidazole-sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in pepper and strawberry. Plant Pathol. J. 23 (3): 187–192.
19.
Koenraadt H., Somerville S.C., Jones A.L. 1992. Characterization of mutations in the beta-tubulin gene of benomyl-resistant field isolates of Venturia inaequqlis and other plant pathogenic fungi. Phytopathology 82 (11): 1348–1354.
20.
Kumar A.S., Reddy N.P.E., Reddy K.H., Devi M.C. 2007. Evaluation of fungicidal resistance among Colletotrichum gloeosporioides isolates causing mango anthracnose in Agri Export Zone of Andhra Pradesh, India. Plant Pathol. Bull. 16 (3): 157–160.
21.
Kuo K.-C. 2001. Sensitivity of mango anthracnose pathogen, Colletotrichum gloeosporioides, to the fungicide prochloraz in Taiwan. Proc. Natl. Sci. Counc. ROC(B) 25 (3): 174–179.
22.
Ma Z., Michailides T.J. 2005. Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Protect. 24 (10): 853–863.
23.
Ma Z., Yoshimura M.A., Michailides T.J. 2003. Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California. Appl. Environ. Microbiol. 69 (12): 7145–7152.
24.
Maymon M., Zveibil A., Pivonia S., Minz D., Freeman S. 2006.Identification and characterization of benomyl-resistant and -sensitive populations of Colletotrichum gloeosporioides from Statice (Limonium spp.). Phytopathology 96 (5): 542–548.
25.
Mills P.R., Sreenivasaprasad S., Brown A.E. 1992. Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiol. Lett. 98 (1–3): 137–144.
26.
Orbach J.M., Porro E.B., Yanofsky C. 1986. Cloning and characterization of the gene for β-tubulin from a benomyl-resistant mutant of Neurospora Grassa and its use as a dominant selectable marker. Mol. Cell. Biol. 6 (7): 2452–2461.
27.
Peres N.A.R., Souza N.L., Peever T.L., Timmer L.W. 2004. Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus. Plant Dis. 88 (2): 125–130.
28.
Ploetz R.C. 2003. Diseases of mango. p. 327–363. In: “Diseases of Tropical Fruit Crops” (R.C. Ploetz, ed.). CABI Publishing, Oxford, UK, 544 pp.
29.
Prabakar K., Raguchander T., Saravanakumar D., Muthulakshmi P., Parthiban V.K., Prakasam V. 2008. Management of postharvest disease of mango anthracnose incited by Colletotrichum gloeosporioides. Arch. Phytopathol. Plant Protect. 41 (5): 333–339.
30.
Prakash O. 2004. Diseases and disorders of mango and their management. p. 511–619. In: “Diseases of Fruits and Vegetables” (S.A.M.H. Naqvi, ed.). Vol I. Kluwer Academic Publishers, the Netherlands, 704 pp.
31.
Prior C., Elango F., Whitwell A. 1992. Chemical control of Colletotrichum infection in mangoes.p. 326–336. In: “Collectotrichum:Biology, Pathology and Control” (J.A. Bailey, M.J. Jeger, eds.). CAB International, Oxon, UK, 388 pp.
32.
Ru-Lin Z., Jun-Sheng H. 2007. Cloning of a carbendazim-resistant gene from Colletotrichum gloeosporioides of mango in South China. Afr. J. Biotechnol. 6 (2): 143–147.
33.
Sanders G.M., Korsten L., Wehner F.C. 2000. Survey of fungicide sensitivity in Colletotrichum gloeosporioides from different avocado and mango production areas in South Africa. Eur. J. Plant Pathol. 106 (8): 745–752.
34.
Sariah M. 1989. Detection of benomyl resistance in the anthracnose pathogen, Colletotrichum capsici. J. Islamic Acad. Sci.2 (3): 168–171.
35.
Sholberg P.L., Harlton C., Haag P., Lévesque C.A., O’Gorman D., Seifert K. 2005. Benzimidazole and diphenylamine sensitivity and identity of Penicillium spp. that cause postharvest blue mold of apples using β-tubulin gene sequences. Postharv. Biol. Technol. 36 (1): 41–49.
36.
Singh S.B., Mukherjee I., Maisnam J., Kumar P., Gopal M., Kulshrestha G. 2008. Determination of pesticide residues in IPM and non-IPM sample of mango (Mangifera indica).J. Environ. Sci. Health, Part B. 43 (4): 300–306.
37.
Staub T. 1991. Fungicide resistance: practical experience with antiresistance strategies and the role of integrated use. Annu. Rev. Phytopathol. 29: 421–442.
38.
Steffens J.J., Pell E.J., Tien M. 1996. Mechanisms of fungicide resistance in phytopathogenic fungi.Curr. Opin. Biotechnol. 7 (3): 348–355.
39.
Sutton B.C. 1992. The genus Glomerella and its Anamorph Colletotrichum. p. 1–27. In:“Colletotrichum: Biology, Pathology and Control” (J.A. Bailey, M.J. Jeger, eds.). CAB International, Wallingford, UK, 388 pp.
40.
Taggart P.J., Locke T., Phillips A.N., Pask N., Hollomon D.W., Kendall S.J., Cooke L.R., Mercer P.C. 1999. Benzimidazole resistance in Rhynchosporium secalis and its effect on barley leaf blotch control in the UK. Crop Protect.18 (4): 239–243.
41.
Thapinta A., Hudak P.F. 2000. Pesticide use and residual occurrence in Thailand. Environ. Monit. Assess. 60 (1): 103–114.
42.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25 (24): 4876–4882.
43.
White T.J., Bruns S.L., Taylor J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. p. 315–322. In: “PCR Protocols: A Guide to Methods and Applications” (M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White, eds.). Academic, New York, 482 pp.
44.
Yan K., Dickman M.B. 1996. Isolation of a β-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance. Appl. Environ. Microbiol. 62 (8): 3053–3056.
45.
Yarden O., Katan T. 1993. Mutations leading to substitutions at amino acids 198 and 200 of β-tubulin that correlates with benomyl-resistance phenotypes of field isolates of Botrytis cinerea. Phytopathology 83 (12): 1478–1483.
46.
Yenjit P., Intanoo W., Chamswarng C., Siripanich J., Intana W. 2004. Use of promising bacterial strains for controlling anthracnose on leaf and fruit of mango caused by Colletotrichum gloeosporioides. Walailak J. Sci. Technol. 1 (2): 56–69.
47.
Yoon C.S., Ju E-H., Yeoung Y.R., Kim B.S. 2008. Survey of fungicide resistance for chemical control of Botrytis cinerea on paprika. Plant Pathol. J. 24 (4): 447–452.
48.
Ziogas B.N., Nikou D., Markoglou A.N., Malandrakis A.A., Vontas J. 2009. Identification of a novel point mutation in the β-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay. Eur. J. Plant. Pathol. 125 (1): 97–107.
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