ORIGINAL ARTICLE
Evaluating sensitivity of Pyricularia oryzae in Mekong Delta (Vietnam) to fungicides and effect of Ag/SiO2 nanocomposites on chemical resistance isolates
1
Plant Protection, Nong Lam University Ho Chi Minh City, Ho CHi Minh, Viet Nam
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-09-17
Acceptance date: 2024-11-04
Online publication date: 2025-09-17
Corresponding author
HIGHLIGHTS
- A total of 30 P. oryzae isolates were collected in regions of Mekong Delta, Vietnam
- The resistant P. oryzae to azoxystrobin and chlorothalonil was highlighted
- Ag/SiO2 exhibited a high inhibition effect for mycelial growth of resistant isolates
- Ag/SiO2 activate the hyphae of P.oryzae break filament and damage cell wall integrity
KEYWORDS
TOPICS
ABSTRACT
Solving the fungicide resistance of the rice blast fungus Pyricularia oryzae (P. oryzae) is essential
for rice production in the Mekong Delta region of Vietnam. Thus, this study aimed
to investigate fungicide resistance and evaluate Ag/SiO2 nanocomposites for controlling
the chemical resistance of P. oryzae. Here, a total of 30 P. oryzae isolates was collected,
and most of the isolates exhibited conidia with pyriform and short pyriform. The sensitivity
in vitro of all isolates was measured against those three chemicals via the poisoning
method. Furthermore, the EC50 and the resistance factor (RF) were evaluated for
12 days after inoculation. The obtained results revealed that all of the P. oryzae isolates were
sensitive to tricyclazole; to chlorothalonil, 67% of them were sensitive, while the rest 33%
were medium-sensitive. To azoxystrobin, 17% of them were sensitive, 57% were medium-
-sensitive, 23% were resistant, and the rest, 3%, were highly resistant. Subsequently, the
antifungal activity of the Ag/SiO2 nanocomposites against two P. oryzae resistance isolates
(labeled TM4 and TAT2) was determined. Interestingly, Ag/SiO2 nanocomposites exhibited
a 100% inhibition effect for mycelial growth of TM4 and TAT2 isolates at a concentration of
60 μg ∙ ml–1 because Ag/SiO2 nanocomposites can activate the hyphae of P. oryzae to lose
their typical structure (breaking filaments and damaging cell wall integrity). Overall, this
study confirms the resistance of P. oryzae isolates to azoxystrobin and chlorothalonil and
also highlights the potential of Ag/SiO2 as an alternative solution to control blast rice
disease.
ACKNOWLEDGEMENTS
The research was conducted with the financial support
of the Nong Lam University Ho Chi Minh City (NLU)
under Grant No CS-CB23-NH-01.
RESPONSIBLE EDITOR
Iwona Adamska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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