Mutation at Codon 198 of Tub2 gene for carbendazim resistance in Colletotrichum gloeosporioides causing mango anthracnose in Thailand
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Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University Chiang Mai 50200 and Center of Excellence on Agriculture Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand
Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan
Corresponding author
Pornprapa Kongtragoul
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University Chiang Mai 50200 and Center of Excellence on Agriculture Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand
Journal of Plant Protection Research 2011;51(4):377-384
Screening of field isolates of Colletotrichum gloeosporioides from various mango cultivars from markets and orchards in Thailand identified 113 carbendazim-resistant isolates. Isolates with a highly-resistant phenotype (HR) grew well on Potato Dextrose Agar (PDA) amended with carbendazim even at ≥500 mg/l. Isolates with carbendazim-resistant phenotype had a conspicuous mutation at a particular site in the β-tubulin ( TUB2 ) gene sequence. The sequence of TUB2 in HR isolates showed a single nucleotide transversion of adenine to cytosine, resulting in a substitution at codon 198 from glutamic acid (GAG) in wild type to alanine (G CG) in HR isolates. This is the first report of the molecular determination of field isolates for benzimidazole fungicide resistance in Southeast Asia in C. gloeosporioides causing mango anthracnose.
The authors have declared that no conflict of interests exist.
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