ORIGINAL ARTICLE
Antifungal activities of bacteria producing bioactive compounds isolated from rice phyllosphere against Pyricularia oryzae
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia
2
Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University, Indonesia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-08-17
Acceptance date: 2019-03-27
Online publication date: 2019-04-12
Corresponding author
Aris Tri Wahyudi Tri Wahyudi
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia
Journal of Plant Protection Research 2019;59(1):86-94
KEYWORDS
TOPICS
ABSTRACT
Rice blast is the main disease of rice plants in Indonesia and several countries worldwide.
Controlling this disease using chemical fungicides has harmful effects on the environment.
Therefore, we need biocontrol agents which are more environmentally friendly such as rice
phyllosphere bacteria. This study aimed to explore bacteria producing bioactive compounds
from the rice phyllosphere environment to control blast disease. A total of 88 isolates
were successfully isolated from rice leaves in Sukabumi, Situgede, and Jasinga (West Java,
Indonesia). From them, we obtained 22 bacteria isolates with antifungal activity against
Pyricularia oryzae in vitro assay. In addition, seven non-pathogenic bacteria were obtained
from further screening in hypersensitivity, hemolysis and pathogenicity assays, namely
STGG 3, STGG 7, STGG 8, STGG 14, SKBV 1, STGV 8, and SKBG 78. To show their
antifungal activity, we tested crude extracts of these seven isolates and the results revealed
that all the crude extracts can inhibit the growth of P. oryzae. Based on a genetic approach,
isolates STGG 3, STGG 7, and STGG 14 were found to have both nonribosomal peptide
synthetases (NRPS) and polyketide synthases (PKS) genes, while isolate SKBV 1 only had
the NRPS gene. The NRPS and PKS genes from potential isolates were similar to NRPS and
PKS genes of Bacillus sp. in different strains. Furthermore, molecular identification based
on the 16S rRNA gene revealed that the seven potential isolates belong to three genera,
i.e. Bacillus (STGG 3, STGG 7, STGG 8, STGG 14, SKBV 1), Enterobacter (STGV 8) and
Brachybacterium (SKBG 78). We suggest that the seven isolates found in this study have
potency and could be recommended as biocontrol agents of blast disease caused by P. oryzae.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (25)
1.
Amoutzias G.D., Peer Y.V., Mossialos D. 2008. Evolution and taxonomic distribution of nonribosomal peptide and polyketide synthases. Future Microbiology 3 (3): 361–370. DOI: http://doi.org/10.2217/1746091....
2.
Bernal M.G., Cordova C.A.I., Saucedo P.E., Gonzalez M.C., Marrero R.M., Suastegui M.J.M. 2015. Isolation in vitro selection of actinomycetes strains as potential probiotics for aquaculture. Veterinary World 8 (2): 170–176. DOI: https://doi.org/10.14202/vetwo....
3.
Chowdhury S.P., Hartman A., Gao X., Borriss R. 2015. Biocontrol mechanism by root-associated Bacillus amyloliquifaciens FZB42-a review. Frontiers in Microbiology 6 (780): 1–11. DOI: http://doi.org/10.2436/20.1501....
4.
Fisch K.M. 2013. Biosynthesis of natural products by microbial iterative hybrid PKS-NRPS. Royal Society of Chemistry Advances 3: 18228–18247. DOI: http://doi.org/10.1039/c3ra426....
5.
Fredriksson N.J., Hermansson M., Wilen B. 2013. The choice of PCR primers has great impact on assessments of bacterial community diversity and dynamics in a wastewater teatment plant. Plos One 8 (10): 1–19. DOI: http://doi.org/10.1371/journal....
6.
Gontia I., Kavita K., Schmid M., Hartmann A., Jha B. 2011. Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential. International Journal of Systematic and Evolutionary Microbiology 61 (12): 2799–2804. DOI: https://doi.org/10.1099/ijs.0.....
7.
Harsonowati W., Astuti R.I., Wahyudi A.T. 2017. Leaf blast disease reduction by rice-phyllosphere actinomycetes producing bioactive compounds. Journal of General Plant Pathology 83 (2): 98–108. DOI: https://doi.org/10.1007/s10327....
8.
Islam M.R., Jeong Y.T., Lee Y.S., Song C.H. 2012. Isolation and identification of antifungal compounds from Bacillus subtilis C9 inhibiting the growth of plant pathogenic fungi. Mycobiology 40 (1): 59–66. DOI: http://dx.doi.org/10.5941/MYCO....
9.
Junaid M.J., Dar N.A., Bhat T.A., Bhat A.H., Bhat M.A. 2013. Commercial biocontrol agents and their mechanism of action in the management of plant pathogens. International Journal of Modern Plant & Animal Sciences 1 (2): 39–57.
10.
Kandel S.L., Firrincieli A., Joubert P.M., Okubara P.A., Leston N.D., McGeorge K.M., Mugnoza G.S., Herfouche A., Kim S., Doty S.L. 2017. An in vitro study of bio-control and plant growth promotion potential of Salicaceae endophytes. Frontiers in Microbiology 8 (386): 1–16. DOI: http://doi.org/10.3389/fmicb.2....
11.
Kharisma S.D., Cholil A., Aini L.Q. 2013. Resilience of several genotypes of hybrid rice (Oryza sativa L.) against Pyricularia oryzae cav. rice leaf blast disease causes. Jurnal Hama Penyakit Tanaman 1 (2): 19–27.
12.
Khrisanti N.P.R.A, Nawangsih A.A, Wahyudi A.T. 2015. Nonpathogenic phyllosphere bacteria producing bioactive compounds as biological control of Xanthomonas oryzae pv. oryzae. International Journal of Pharmaceutical and Biological Sciences 6 (1): 801–810.
13.
Liu W., Wong C., Chung K.M., Jiang J., Leung F.C. 2013. Comparative genome analysis of Enterobacter cloacae. PlosOne 8 (9): 1–15. DOI: http://doi.org/10.1371/journal....
14.
Lou C., Zhou H., Zou J., Wang X., Zhang R., Xiang Y., Chen Z. 2015. Bacillomycin L and surfactin contribute synergistically to the phenotypic features of Bacillus subtilis 916 and the biocontrol of rice sheath blight induced by Rhizoctonia solani. Applied Microbiology and Biotechnology 99 (4): 1897–1910. DOI: https://doi.org/10.1007/s00253....
15.
Mora I., Cabrefiga J., Montesinos E. 2011. Antimicrobial peptide genes in Bacillus strains from plant enviroments. International Microbiology 14 (4): 213–223. DOI: https://doi.org/10.2436/20.150....
16.
Rastogi G., Coaker G.L., Leveau J.H.J. 2013. New insights into the structure and function of phyllosphere microbiota through high-throughput molecular approaches. Federation of European Microbiological Societies Microbiology Letters 348: 1–10. DOI: http://doi.org/10.1111/1574-69....
17.
Rungjindamai N. 2016. Isolation and evaluation of biocontrol agents in controlling anthracnose disease of mango in Thailand. Journal of Plant Protection Research 56 (3): 306–311. DOI: http://doi.org/10.1515/jppr-20....
18.
Sha Y., Wang Q., Li Y. 2016. Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast. SpringerPlus 5 (1238): 1–13. DOI: http://doi.org/10.1186/s40064-....
19.
Sukanya S.L., Yamini D., Fathima S.K. 2011. Eco-friendly management of Pyricularia oryzae: the causal agent of blast of paddy. Current Botany 2 (8): 46–49.
20.
Sundh I., Hokeberg M., Levenfors J.J., Nilsson A.I. 2011. Safety assessment of biocontrol and plant growth-promoting Psedomonads useful in crop production. Annals of Applied Biology 159 (2): 291–301. DOI: http://doi.org/10.1111/j.1744-....
21.
Tambadou F., Lanneluc I., Sablé S., Klein G.L., Doghri I., Sopéna V., Didelot S., Barthélémy C., Thiéry V., Chevrot R. 2014. Novel nonribosomal peptide synthetase (NRPS) genes sequenced from intertidal mudflat bacteria. Federation of European Microbiological Societies Research Letter 357 (2): 123–130. DOI: http://doi.org/10.1111/1574-69....
22.
Walia N.K., Cameotra S.S. 2015. Lipopeptides: biosynthesis and application. Journal of Microbial and Biochemical Technology 7 (2): 103–107. DOI: http://doi.org/10.4172/1948-59....
23.
Yadav R.K.P., Karamanoli K., Vokou D. 2010. Estimating bacterial population on the phyllosphere by serial dilution plating and leaf imprint methods. Ecoprint: An International Journal of Ecology 17: 47–52. DOI: http://doi.org/10.3126/eco.v17....
24.
Zhang W., Zhang F., Li Z., Miao X., Meng Q., Zhang X. 2009. Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China sea sponges. Journal of Applied Microbiology 107: 567–575. DOI: http://doi.org/10.1111/j.1365-....
25.
Zhao X., Kuipers P. 2016. Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species. BioMed Central Genomics 17 (882): 1–18. DOI: http://doi.org/10.1186/s12864-....
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
