ORIGINAL ARTICLE
Plant growth promoting rhizobacteria of cotton affecting the developmental stages of Helicoverpa armigera
 
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Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
 
 
Corresponding author
Ayyathurai Vijayasamundeeswari
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University Coimbatore – 641 003, Tamil Nadu, India
 
 
Journal of Plant Protection Research 2009;49(3):239-243
 
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ABSTRACT
Rhizobacteria isolated from cotton roots exhibiting antagonism towards seedling blight and leaf blight of cotton were tested for their efficiency against the insect pest American bollworm (Helicoverpa armigera). The bioformulation developed using Bacillus subtilis (isolate DGL9) + chitin was found to be detrimental to the developmental stages of H. armigera (larva, pupa and adult) by causing larval mortality, pupal and adult malformation with reduced adult emergence. Generally, the larvae exhibited antifeeding behaviour when fed on bolls collected from rhizobacterial treatments. Hence, the developmental stages were altered leading to early pupation. Further, the efficacy of the isolate DGL9 was confirmed by culturing the bacteria in a suitable medium and incorporating the cell suspension and supernatant obtained form the broth culture in larval diet. The larvae fed to the diet exhibited defective developmental stages which was more significant in case of diet incorporated with supernatant. The percentage of pupal malformation, adult emergence and adult malformation was high at 96 h of incubation with the supernatant.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (23)
1.
Bhuvaneswari R. 2005. Endophytic Bacillus mediated induced systemic resistance against bacterial blight (Xanthomonas axonopodis pv. malvacearum) and bollworm (Helicoverpa armigera) in cotton. M.Sc. (Ag.) Thesis, Tamil Nadu Agricutlural University, Coimbatore-3, India, 86 pp.
 
2.
Bong C.F.J., Sikorowski P.P. 1991. Effects of cytoplasmic polyhedrosis virus and bacterial contamination on growth and development of corn earworm, Heliothis zeae(Boddie). J. Invertebr. Pathol. 57: 406–412.
 
3.
Fahey I.W., Dimolk M.B., Tomasino S.F., Taylor l.M. Carlson P.S. 1991. Genetically engineered endophytes as biocontrol agent: a case study from industry. p. 401–411. In: “Microbial Ecology of Leaves” (J.H. Andrews, S.S. Hirano, eds.). Springer Verlag, Berlin.
 
4.
Filt G.P. 1989. The ecology of Heliothis species in relation to agro ecosystems. Annu. Rev. Entomol. 34: 17–52.
 
5.
Jayaswal A.T. 1989. Management of American bollworm on cotton in Andra Pradesh. Indian Farming 7: 6–17.
 
6.
Kloepper J.W. Beauchamp C.J. 1992. A review of issues related to measuring colonization of plant roots by bacteria. Can. J. Microbiol. 38: 1219–1232.
 
7.
Mathews M. 1991. Classification of Heliothinae. Bull. No. 44, Natural Res. Instt., Chattam, United Kingdom, 198 pp.
 
8.
Qingwen Z., Ping L., Gang W., Qingnian C. 1998. On the biochemical mechanism of induced resistance of cotton to cotton bollworm by cutting off young seedling at plumular axis. Acta Phytolacca Sinica 25: 209–212.
 
9.
Racke J., Sikora R.A. 1992. Isolation, formulation and antagonistic activity of rhizobacteria towards the potato cyst nematode, Globodera pallida. Soil Biol. Biochem. 24: 521–526.
 
10.
Radjacommare R. 2000. Pseudomonas fluorescens mediated systemic resistance in rice against sheath blight disease and leaffolder insect. M.Sc. (Ag.,) Thesis, Tamil Nadu Agricultural University, Coimbatore, India, 119 pp.
 
11.
Radjacommare R., Nandakumar R., Kandan A., Suresh S., Bharathi M., Raguchander T., Samiyappan R. 2002. Pseudomonas fluorescens based bioformulation for the management of sheath blight and leaffolder in rice. Crop Prot. 21: 671–677.
 
12.
Rajendran L. 2003. Bacterial endophytes mediated induced systemic resistance against major pests and diseases in cotton. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore, India, 95 pp.
 
13.
Reed W., Pawar C.S. 1982. Heliothis: A global problem. p. 529–535. In: Proceedings of International Workshop on “Heliothis management” (W. Reed, V. Kumbek, eds.). Patancheru, AP,.India, November 15–20, 1981, ICRISAT Center.
 
14.
Regupathy A., Kranthi K., Singh J., Iquabal A., Wu Y., Russel D.Patterns and magnitude of insecticide resistance levels in India, Pakistan and China. p. 115–129. In: Proceedings of World Cotton Conference 3. Cape Town, South Africa, March 9–13, 2003.
 
15.
Regupathy A., Manoharan J., Ashokan G., Soundararajan A.P. 1997. Synergistic suppression of fenvalerate resistance in field population of Helicoverpa armigera in Tamil Nadu. Resist. Pest Mgmt. 9: 23–25.
 
16.
Regupathy A., Palanisamy S., Chandramohan N., Gunathilagaraj K. 1998. A guide on crop pests. Sooriya Desktop Publisher, Coimbatore, India: 38–39.
 
17.
Regupathy A., Rajavel D.S., Rajkumar S., Russel D. 1999. Present status of insecticides resistance in Helicoverpa armigera and its management in Tamil Nadu, India. p. 8–55. In: Proceedings of ICAC-CCRI, Regional Consultation Insecticide Resistance Management in Cotton. Multan, Pakistan, June 28–July 1, 1999.
 
18.
Sathiah N. 2001. Studies on improving production and formulation of the nuclear polyhedrosis virus of cotton bollworm, Helicoverpa armigera(Hubner). Ph.D. Thesis, Tamil Nadu Agricultural University, Coimbatore, India, 174 pp.
 
19.
Vidhyasekaran P., Muthamilan M. 1995. Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis. 79: 782–786.
 
20.
Yaman M., Demirbag Z., Belduz A.O. 1999. Investigations on the bacterial flora as a potential biocontol agent of chestnut weevil, Curculio elephas (Coleoptera: Curculionidae) in Turkey. Afr. J. Mycol Biotechnol.54: 625–630.
 
21.
Zehnder G.W., Kloepper J., Yao C., Wei G. 1997a. Induction of systemic resistance against cucumber beetles (Coleoptera: Chrysomelidae) by plant growth – promoting rhizobacteria. J. Econ. Entomol. 90: 91–396.
 
22.
Zehnder G.W., Kloepper J.W., Tuzun S., Yao C., Wei G., Chambliss O., Shelby R. 1997b. Insect feeding on cucumber mediated by rhizobacteria-induced plant resistance. Entomol. Exp. Appl. 83: 81–85.
 
23.
Zehnder G.W., Murphy J.F., Sikora E.J., Kloepper J.W. 2001. Application of rhizobacteria for induced resistance. Eur. J. Plant Pathol. 107: 39–50.
 
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