ORIGINAL ARTICLE
Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest
| 1 | Department of Biochemistry, Taurida Academy of V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea |
| 2 | Department of Biochemistry, Medical Academy of V.I. Vernadsky Crimean Federal University, 295051 Simferopol, Crimea |
| 3 | All-Russian Research Institute for Silviculture and Mechanization of Forestry, 141200 Pushkino, Russia |
| 4 | Department of Virology, Biological Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia |
| 5 | Genetics Department of Russian Centre of Forest Health, 141207 Pushkino, Russia |
| 6 | Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia |
| 7 | Center of Bioengineering, Russian Academy of Sciences, 117312 Moscow, Russia |
CORRESPONDING AUTHOR
Palmah M. Nyadar
Department of Biochemistry, Taurida Academy of V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea
Department of Biochemistry, Taurida Academy of V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea
Submission date: 2016-09-28
Acceptance date: 2017-01-30
Journal of Plant Protection Research 2017;57(1):18–24
KEYWORDS
baculoviral infectionDNA insecticidesDNA oligonucleotidesforest and crop protectiongypsy mothIAP genes
TOPICS
ABSTRACT
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV-infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by the antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant
protection against insect pests.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (37)
1.
Agol V.I. 1997. How viruses cause disease. Soros’s Educational Magazine 9: 27. Bakhvalov S.A., Martem’yanov V.V., Bakhvalova V.N., Morozova O.V. 2012. Detection of the nuclear polyhedrosis virus DNA in samples from eggs and caterpillars at different stages of the gypsy moth Lymantria dispar (L.) population dynamics. Voprosy Virusologii 57 (4): 35–37.
2.
Bertin J., Mendrysa S.M., La Count D.J., Gaur S., Krebs J.F., Armstrong R.C., Tomaselli K.J., Friesen P.D. 1996. Apoptotic supression by baculovirus P35 involves cleavage by and inhibition of a virus induced CED – 3/ICE-like protease. Journal of Virology 70 (9): 6251–6259.
3.
Cerio R.J., Vandergaast R., Friesen P.D. 2010. Host insect inhibitor-of-apoptosis SfIAP functionally replaces baculovirus IAP but is diff erentially regulated by its N-terminal leader. Journal of Virology 84 (21): 11448–11460.
4.
Clarke T.E., Clem R.J. 2003. Insect defenses against virus infection: the role of apoptosis. International Reviews of Immunology 22 (5–6): 401–424.
5.
Clem R.J., Passarelli L.A. 2013. Baculoviruses: Sophisticated Pathogens of Insects. PLoS Pathogens. Available on: http://dx.doi.org/10.1371/jour.... [Accessed: November 14, 2013].
6.
Clem R.J. 2015. Viral IAPs, then and now. Seminars in Cell & Developmental Biology 39: 72–79.
7.
Duan L., Otvos I.S., Xu L.B., Conder N., Wang Y. 2011. Comparison of the activities of three LdMNPV isolates in the laboratory against the Chinese strain of Asian gypsy moth. Open Entomology Journal 5 (1): 24–30.
8.
Dias N., Stein C.A. 2002. Antisense oligonucleotides: basic concepts and mechanism. Molecular Cancer Therapeutics 1 (5): 347–355.
9.
Ebling P.M., Otvos I.S., Conder N. 2004. Comparative activity of three isolates of LdMNPV against two strains of Lymantria dispar. Canadian Entomologist 136 (5): 737–747.
10.
Gu L., Knipple D.C. 2013. Recent advances in RNA interference research in insects: Implications for future insect pest management strategies. Crop Protection 45: 36–40.
11.
Hughes A.L. 2002. Evolution of inhibitors of apoptosis in baculoviruses and their insect hosts infection. Infection, Genetics and Evolution 2 (1): 3–10.
12.
Ikeda M., Yamada H., Ito H., Kobayashi M. 2011. Baculovirus IAP1 induces caspase-dependent apoptosis in insect cells. Journal of General Virology 92 (11): 2654–2663.
13.
Ikeda M., Yamada H., Hamajima R., Kobayashi M. 2013. Baculovirus genes modulating intracellular innate antiviral immunity of lepidopteran insect cells. Virology 435 (1): 1–13.
14.
Jehle J.A., Blissard G.W., Bonning B.C., Cory J.S., Herniou E.A., Rohrmann G.F., Theilmann D.A., Th iem S.M., Vlak J.M. 2006. On the classifi cation and nomenclature of baculoviruses: a proposal for revision. Archives of Virology 151 (7): 1257–1266.
15.
Lozowicka B., Hrynko I., Kaczyński P., Rutkowska E., Jankowska M., Mojsak P. 2015. Occurrence of pesticide residues in fruit from Podlasie (Poland) in 2012. Journal of Plant Protection Research 55 (2): 142–145.
16.
Lundgren J.G., Duan J.J. 2013. RNAi-based insecticidal crops: potential eff ects on non-target species. BioScience 63 (8): 657–665.
17.
Manji G.A., Hozar R.R., La Count D.J., Friesen P.D. 1997. Baculovirus inhibitor of apoptosis functions at or upstream of the apoptotic supressor P35 to prevent programmed cell death. Journal of Virology 71 (6): 4509–4516.
18.
Miller L.K., Lingg A.J., Bulla L.A.J. 1983. Bacterial viral and fungal insecticides. Science 219 (4585): 715–721.
19.
Mitchell J.K., Friesen P.D. 2012. Baculoviruses modulate a proapoptotic DNA damage response to promote virus multiplication. Journal of Virology 86 (24): 13542–13553.
20.
Oberemok V.V. 2008a. Method of elimination of phyllophagous insects from order Lepidoptera. Patent 36445 Ukraine, the applicant and the owner of the patent Taurida National V.I Vernadsky University. № u 2008 0674.
21.
Oberemok V.V. 2008b. [Proof of transovarial transmission of Lymantria dispar nucleopolyhedrovirus (Fam. Baculoviridae) with the RAPD-PCR method.] Zhurnal Obshcheo Biologii 69 (5): 397–400. (in Russian).
22.
Oberemok V.V. 2011. DNA markers in the study of the relationship between nuclear polyhedrosis virus and its host Lymantria dispar. Summary of Ph.D. thesis. Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, 22 pp. (in Ukrainian).
23.
Oberemok V.V., Skorokhod O.A. 2014. Single-stranded DNA fragments of insect-specific nuclear polyhedrosis virus act as selective DNA insecticides for gypsy moth control. Pesticide Biochemistry and Physiology 113: 1–7.
24.
Oberemok V.V., Laikova K.V., Gninenko Yu.I., Zaitsev A.S., Nyadar P.M., Adeyemi T.A. 2015a. A short history of insecticides. Journal of Plant Protection Research 55 (3): 221–226.
25.
Oberemok V.V., Zaitsev A.S., Levchenko N.N., Nyadar P.M. 2015b. A brief review of most widely used modern insecticides and prospects for the creation of DNA insecticides. Entomological Review 95 (7): 824–831.
26.
Oberemok V.V., Nyadar P.M. 2015. Investigation of mode of action of DNA insecticides on the basis of LdMNPV IAP3 gene. Turkish Journal of Biology 39: 258–264.
27.
Oberemok V.V., Laikova K.V., Zaitsev A.S., Gushchin V.A., Skorokhod O.A. 2016. The RING for gypsy moth control: topical application of fragment of its nuclear polyhedrosis virus anti-apoptosis gene as insecticide. Pesticide Biochemistry and Physiology 131: 32–39.
28.
Simchuk A.P., Oberemok V.V., Ivashov A.V. 2012. Genetics of interactions among moths, their host plants and enemies in crimean oak forests, and its perspective for their control. p. 187–205. In: “Moths: Types, Ecological Signifi cance and Control” (L. Cauterruccio, ed.) Nova Science Publisher, N.Y., USA, 286 pp.
29.
Srinivasula S.M., Ashwell J.D. 2008. IAPs: what’s in a name? Molecular Cell 30 (2): 123–135.
30.
Terenius O., Papanicolaou A., Garbutt J.S., Eleft herianos I., Huvenne H., Kanginakudru S., Albrechtsen M., An C., Aymeric J.L. 2011. RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. Journal of Insect Physiology 57 (2): 231–245.
31.
Thorp H.H. 2000. The importance of being r: greater oxidative stability of RNA compared with DNA. Chemical Biology 7 (2): 33–36.
32.
Vandergaast R., Schultz K.L., Cerio R.J., Friesen P.D. 2011. Active depletion of host cell inhibitor-of-apoptosis proteins triggers apoptosis upon baculovirus DNA replication. Journal of Virology 85 (16): 8348–8358.
33.
Vandergaast R., Mitchell J.K., Byers N.M., Friesen P.D. 2015. Insect inhibitor of apoptosis iaps are negatively regulated by signal-induced N-terminal degrons absent within viral IAPs. Journal of Virology 89 (8): 4481–4493.
34.
Vanyushin B.F. 2001. Apoptosis of plants. Uspehi Biologicheskoi Khimii 41: 3–38. (in Russian).
35.
Weitzman M.D., Lilley C.E., Chaurushiya M.S. 2010. Genomes in conflict: maintaining genome integrity during virus infection. Annual Review Microbiology 64: 61–81.
36.
Yamada H., Shibuya M., Kobayashi M., Ikeda M. 2012. Baculovirus Lymantria dispar multiple nucleopolyhedrovirus IAP2 and IAP3 do not suppress apoptosis, but trigger apoptosis of insect cells in a transient expression assay. Virus Genes 45 (2): 370–379.
37.
Yamada H., Shibuya M., Kobayashi M., Ikeda M. 2011. Identification of a novel apoptosis suppressor gene from the baculovirus Lymantria dispar multi capsid nucleopolyhedrovirus. Journal of Virology 85 (10): 5237–5242.
RELATED ARTICLE
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.