The indiscriminate use of chemical pesticides to control insect pests has caused various ecological and human health hazards. This study evaluated the potential of indigenous isolates of entomopathogenic fungi (EPFs) as an eco-friendly insecticidal alternative against fruit flies (Bactrocera dorsalis). Eleven isolates of Beauveria bassiana and a single isolate of Metarhizium anisopliae were obtained from soil samples from different areas using the Galleria-bait technique. Pathogenicity tests against Galleria mellonella larvae (at 1×10⁵ conidia·ml⁻¹) identified three most effective isolates (BbaRuNPC-1-2, BbaPaNPC-3-8, and BbaNwNPC-1-4) for virulence testing against B. dorsalis larvae in vitro through contact application. Probit regression studies showed that the isolate BbaNwNPC-1-4 was the most effective, causing 90% mortality with a lethal time (LT50) value of 6.29 days and a lethal concentration (LC50) of 1.89*103 conidia.ml-1 followed by BbaRuNPC-1-2 (86.67%) and BbaPaNPC-3-8 (76.67%) with LT50 values of 11.67 and 12.46 days, respectively. The isolate BbaPaNPC-3-8 had the lowest LC50 value of 1.05*103 conidia.ml-1 making it 1.18 times and 3.74 times more lethal than the isolates BbaNwNPC-1-4 and BbaRuNPC-1-2 with values 1.89*103 and 3.93*103 conidia . ml-1, respectively. These findings indicate that B. bassiana isolates, particularly BbaNwNPC-1-4 for rapid mortality and BbaPaNPC-3-8 for concentration efficiency, could offer promising biological control alternatives for management of B. dorsalis in integrated pest management programs.