Biochemical characterisation of the tissue degrading enzyme, collagenase, in the spined soldier bug, Podisus maculiventris (Hemiptera: Pentatomidae)
More details
Hide details
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
Submission date: 2013-01-06
Acceptance date: 2014-05-20
Corresponding author
Mahboob Ghamari
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
Journal of Plant Protection Research 2014;54(2):164-170
Podisus maculiventris (Say) is a generalist predator attacking many insect species from different orders. The bug injects saliva into its prey’s body. The ingested hemolymph and liquefied internal tissues pass through the bug’s alimentary tract. Collagenase working on peptide bonds of collagen and basement membrane proteins, leads to the disintegration of the prey’s internal organs. As yet, there is an almost complete lack of knowledge on the collagenase activity in P. maculiventris. The collagenase activity of the salivary glands and midgut was optimum at pH 8.0 which was congruent with the optimal pH of the total proteolytic activity of the salivary glands. More collagenolytic activity was determined in the posterior lobe of the salivary glands and anterior midgut. Significant inhibition of collagenolytic activity by ethylenediaminetetraacetic acid (EDTA) revealed the enzyme is a metalloproteinase. The collagenase activity notably decreased when the bug went hungry. The salivary gland collagenase is a vital enzyme in extra-oral digestion and facilitates the action of other digestive enzymes. The midgut collagenase may be involved in the digestion of the ingested muscle fibers. The collagenase probably acts as an intoxicating agent in the saliva (venom) of P. maculiventris. Paralysing toxins are present in the salivary gland secretion.
The authors have declared that no conflict of interests exist.
Aghaali N., Ghadamyari M., Hosseininaveh V., Riseh N.S. 2013. Protease inhibitor from the crude extract of plant seeds affects the digestive proteases in Hyphantria cunea (Lep.: Arctiidae). J. Plant Prot. Res. 53 (4): 338–346.
Alberts B., Bray D., Lewis J., Raff M., Roberts K., Watson J.D. 2007. Molecular Biology of the Cell. Garland Science, New York, 1408 pp.
Atkinson R.K., Wright L.G. 1991. Studies of the necrotic actions of the venoms of several Australian spiders. Comp. Bioch. Physiol. C. 98 (2): 441–444.
Baptist B.A. 1941. The morphology and physiology of the salivary glands of the Hemiptera-Heteroptera. Qj. Microsc. Sci. 2 (329): 91–139.
Barillas-Mury C.V., Noriega F.G., Wells M.A. 1995. Early trypsin activity is part of the signal transduction system that activates transcription of the late trypsin gene in the midgut of the mosquito. Insect Biochem. Physiol. 25 (2): 241–246.
Bell H.A., Down R.E., Edwards J.P., Gatehouse J.A., Gatehouse A.M.R. 2005. Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae); effect of proteinase inhibitors. Eur. J. Entomol. 102 (2): 139–145.
Bigham M., Hosseininaveh V. 2010. Digestive proteolytic activity in the pistachio green stink bug, Brachynema germari Kolenati (Hemiptera: Pentatomidae). J. Asia-Pacific Entomol. 13 (3): 221–227.
Boulard C., Garrone R. 1978. Characterization of a collagenolytic enzyme from larvae of Hypoderma lineatum. Comp. Biochem. Physiol. B. 59 (3): 251–255.
Bowles V.M., Carnegie P.R., Sandeman R.M. 1988. Characterization of proteolytic and collagenolytic enzymes from the larvae of Lucilia cuprina, the sheep blowfly. Aust. J. Biol. Sci. 41 (2): 269–278.
Cohen A.C. 1993. Organization of digestion and preliminary characterization of salivary trypsin-like enzymes in a predaceous heteropteran, Zelus renardii. J. Insect Physiol. 39 (10): 823–829.
Cohen A.C. 1995. Extra-oral digestion in predaceous terrestrial Arthropoda. Annu. Rev. Entomol. 40 (1): 85–103.
Cohen A.C. 1998. Solid-to-liquid feeding: the inside(s) story of extra-oral digestion in predaceous Arthropoda. Am. Entomol. 44 (2): 103–117.
Corzo G., Adachi-Akahane S., Nagao T., Kusui Y., Nakajima T. 2001. Novel peptide from assassin bugs (Hemiptera: Reduviidae): isolation, chemical and biological characterization. FEBS Lett. 499 (3): 256–261.
Darvishzadeh A., Hosseininaveh V., Ghamari M. 2013. Identification and biochemical characterisation of α-amylase in the alimentary tract of Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Arch. Phytopathol. Plant Prot. 46 (9): 1061–1069.
De Clercq P. 2000. Predaceous Stinkbugs (Pentatomidae: Asopinae). p. 737–789. In: “Heteroptera of economic importance” (C.W. Schaefe, A.R. Panizzi, eds.) CRC Press, Boca Raton, Florida, 2000 pp.
De Clercq P. 2002. Dark clouds and their silver linings: exotic generalist predators in augmentative biological control. Neotrop. Entomol. 31 (2): 169–176.
De Clercq P., Merlevede F., Mestdagh I., Vandendurpel K., Mohaghegh J., Degheele D. 1998. Predation on the tomato looper Chrysodeixis chalcites, (Esper) (Lep.: Noctuidae) by Podisus maculiventris (Say) and Podisus nigrispinus (Dallas) (Het.: Pentatomidae). J. Appl. Entomol. 122 (1–5): 93–98.
Eisen A.Z., Henderson K.O., Jeffrey J.J., Bradshaw R.A. 1973. Collagenolytic protease from the hepatopancreas of the fiddler crab, Uca pugilator. Purification and properties. Biochemistry 12 (9): 1814–1822.
Fialho M.C.Q., Moreira N.R., Zununcio J.C., Ribeiro A.F., Terra W.R., Serrão J.C. 2012. Prey digestion in the midgut of the predatory bug Podisus nigrispinus (Hemiptera: Pentatomidae). J. Insect Physiol. 85 (6): 850–856.
Folin O., Ciocalteu V. 1927. On tyrosine and tryptophane determination in proteins. J. Biol. Chem. 73 (2): 627–650.
Garcıa-Carreno F.L., Dimes N., Haard N. 1993. Substrate-gel electrophoresis for composition and molecular weight of proteases or proteinaceous protease inhibitors. Anal. Biochem. 214 (1): 65–69.
Grant G.A., Sacchettini J.C., Welgus H.G. 1983. A collagenolytic serine protease with trypsin-like specificity from the fiddler crab Uca pugilator. Biochemistry 22 (2): 354–358.
Hong S.J., Chang C.C. 1985. Electrophysiological studies of mycotoxin a, isolated from prairie rattlesnake (Crotalus viridis viridis) venom, on murine skeletal muscles. Toxicon 23 (6): 927–937.
Hosseininaveh V., Bandani A., Azmayeshfard P., Hosseinkhani S., Kazzazi M. 2007. Digestive proteolytic and amylolytic activities in Trogoderma granarium Everts (Dermestidae: Coleoptera). J. Stor. Proc. Res. 43 (4): 515–522.
Lecroisey A., Boulard C., Keil B. 1979. Chemical and enzymatic characterization of the collagenase from the insect Hypoderma lineaturn. Eur. J. Biochem. 101 (2): 385–393.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1): 265–275.
McPherson J.E. 1980. A list of the prey species of Podisus maculiventris (Hemiptera: Pentatomidae). Gt. Lakes Entomol. 13: 18–24.
Oliveira J.A., Oliveira M.G.A., Guedes R.N.C., Soares M.J. 2006. Morphology and preliminary enzyme characterization of the salivary glands from the predatory bug Podisus nigrispinus (Heteroptera: Pentatomidae). Bull. Entomol. Res. 96 (3): 251–258.
Rosen H. 1957. A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. Biophys. 67 (1): 10–15.
Schmidt O.J. 1982. Biochemistry of insect venoms. Annu. Rev. Entomol. 27 (1): 339–368.
Terra W.R., Ferreira C. 1994. Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. B. 109 (1): 1–6.
Van Lenteren J.C., Babendreier D., Bigler F., Burgio G., Hokkanen H.M.T., Kuske S., Loomans A.J.M., Menzler-Hokkanen I., Van Rijn P.C.J., Thomas M.B., Tommasini M.G., Zeng Q.-Q. 2003. Environmental risk assessment of exotic natural enemies used in inundative biological control. BioControl 48 (1): 3–38.
Journals System - logo
Scroll to top