ORIGINAL ARTICLE
 
HIGHLIGHTS
  • Seed-borne pathogens inhibit the germination and seedling growth of safflower.
  • Boron (B) seed treatments have been used for seed health improvement.
  • Primed seeds promoted germination, emergence, and seedling growth of safflower.
  • Primed seeds with 5-10 ppm B reduced infection rate by 73%.
KEYWORDS
TOPICS
ABSTRACT
The objective of this study was to determine the effects of seed treatments with boron on germination, seedling growth, and disinfection of seed-borne pathogens in safflower. Safflower seeds of the Balcı cultivar were treated with solutions of 0, 5, 10, and 20 ppm boron for 6 hours or surface treated with powdery sodium borate (Na2B8O13.4H2O). The experiment was a completely randomized design with eight replications and 25 seeds per replicate. Treated or untreated seeds were germinated between papers and germination characteristics, emergence, seedling growth, and infected seed rate were investigated. The results showed that the peak germination percentage (96.5%) was achieved in seeds primed with 5 ppm B. The mean germination time was significantly reduced, and the germination index reached the maximum value (23.5) in seeds primed with 5 ppm boron. Emergence percentage improved from 76.5% in the control to 87.5% in the seed dressing. The root and shoot length of the primed seeds increased fourfold. Seedling fresh weight was positively affected by the boron treatments, and seeds primed with 20 ppm boron produced the heaviest fresh seedlings. All boron treatments significantly reduced the infection rate by about 73%, but the lowest infection rate (11.5%) was observed in seeds primed with 5 ppm boron. It was concluded that safflower seeds should be primed with 5-10 ppm boron or at least undergo seed dressing with boron to reduce the negative effects of seed-borne pathogens and promote germination and seedling growth.
ACKNOWLEDGEMENTS
This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. The contribution of the authors to the article is equal.
RESPONSIBLE EDITOR
Arkadiusz Artyszak
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (37)
1.
Abdullah S.K., Al-Mosawi K.A. 2010. Fungi associated with seeds of sunflower (Helianthus annuus) cultivars grown in Iraq. Phytopathologia 57: 11–20.
 
2.
Aguado A., Savoie J.M., Chéreau S., Ducos C., Aguilar M., Ferrer N., Aguilar M., Pinson-Gadais L., Richard-Forget F. 2019. Priming to protect maize from Fusarium verticillioides and its fumonisin accumulation. Journal of the Science of Food and Agriculture 99: 64–72. DOI: https://doi.org/10.1002/jsfa.9....
 
3.
Ancy U.A., Latha A., Stanly N.M. 2022. Effect of nutripriming treatments on growth parameters of seedlings in tray nursery of rice: Effect of nutripriming on tray nursery of rice. Journal of AgriSearch 9 (1): 59–62. DOI: https://doi.org/10.21921/jas.v....
 
4.
Arshad Javaid A.A., Akhtar N., Hanif M., Farooq M.A. 2006. Efficacy of some fungicides against seed-borne mycoflora of wheat. Mycopath 4 (1): 45–49.
 
5.
Ashagre H., Hamza I.A., Fita U., Estifanos E. 2014. Boron toxicity on seed germination and seedling growth of safflower (Carthamus tinctorius L.). Herald Journal of Agriculture and Food Science Research 3 (1): 1–6.
 
6.
Bianchini A., Bullerman L.B. 2014. Mycotoxins. p. 854–861. In: “Encyclopedia of Food Microbiology” (C.A. Batt, M.L. Tortorello ML, eds.). Academic Press, Elsevier. DOI: http://dx.doi.org/10.1016/B978....
 
7.
Chakraborty P., Dwivedi P. 2022. Evaluating the effects of borax as priming agent on germination and seedling parameter. International Journal of Agriculture, Environment and Biotechnology 15 (3): 663–667. DOI: https://doi.org/10.30954/0974-....
 
8.
Doolotkeldieva T., Bobusheva S. 2022. Microbial communities of vegetable seeds and biocontrol microbes for seed treatment. Seed Science and Technology 50 (1–2): 77–102. DOI: https://doi.org/10.15258/sst.2....
 
9.
Düzgüneş O., Kesici T., Gürbüz F. 1983. Statistic Methods 1. Ankara University Faculty of Agriculture Publication. (in Turkish).
 
10.
Ergin N., Kulan E.G., Kaya M.D. 2021. The effects of fungicidal seed treatments on seed germination, mean germination time and seedling growth in safflower (Carthamus tinctorius L.). Selcuk Journal of Agriculture and Food Sciences 35 (2): 136–140. DOI: https://doi.org/10.15316/SJAFS....
 
11.
Estevez-Fregoso E., Farfán-García E.D., García-Coronel I.H., Martínez-Herrera E., Alatorre A., Scorei R.I., Soriano-Ursúa M.A. 2021. Effects of boron-containing compounds in the fungal kingdom. Journal of Trace Elements in Medicine and Biology 65: 126714. DOI: https://doi.org/10.1016/j.jtem....
 
12.
Farooq M., Atique-Ur-Rehman, Aziz T., Habib M. 2011. Boron nutripriming improves the germination and early seedling growth of rice (Oryza sativa L.). Journal of Plant Nutrition 34 (10): 1507–1515. DOI: https://doi.org/10.1080/019041....
 
13.
Farooq M., Ullah A., Rehman A., Nawaz A., Nadeem A., Wakeel A., Nadeem F., Siddique K.H. 2018. Application of zinc improves the productivity and biofortification of fine grain aromatic rice grown in dry seeded and puddled transplanted production systems. Field Crops Research 216: 53–62. DOI: https://doi.org/10.1016/j.fcr.....
 
14.
Iqbal S., Farooq M., Cheema S.A., Afzal I. 2017. Boron seed priming improves the seedling emergence, growth, grain yield and grain biofortification of bread wheat. International Journal of Agriculture & Biology 19 (1): 177–182. DOI: https://doi.org/10.17957/IJAB/....
 
15.
Iqbal S., Farooq M., Ullah A., Luqman M., Akram H.M., Munir M.K., Zafar N. 2021. Boron biofortification through seed coating: a way to alleviate malnutrition and to improve grain yield of bread wheat. Pakistan Journal of Agricultural Sciences 58 (5): 1501–1509. DOI: https://doi.org/10.21162/PAKJA....
 
16.
ISTA. 2018. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
 
17.
Kakde R.B., Chavan A.M. 2011. Extracellular lipase enzyme production by seed-borne fungi under the influence of physical factors. International Journal of Biology 3 (1): 94–100. https://doi.org/10.5539/ijb.v3....
 
18.
Kaya M.D., Kulan E.G., Ileri O., Avci S. 2019. Prediction of viability and emergence capacity of safflower seed lots. Journal of Animal & Plant Sciences 29 (3): 714–720.
 
19.
Li Y., Yang Z., Bi Y., Zhang J., Wang D. 2012. Antifungal effect of borates against Fusarium sulphureum on potato tubers and its possible mechanisms of action. Postharvest Biology and Technology 74: 55–61. DOI: http://dx.doi.org/10.1016/j.po....
 
20.
Machado P.P., Steiner F., Zuffo A.M., Machado R.A. 2018. Could the supply of boron and zinc improve resistance of potato to early blight? Potato Research 61: 169–182. DOI: https://doi.org/10.1007/s11540....
 
21.
Majda C., Khalid D., Aziz A., Rachid B., Badr A.S., Lotfi A., Mohamed B. 2019. Nutri-priming as an efficient means to improve the agronomic performance of molybdenum in common bean (Phaseolus vulgaris L.). Science of the Total Environment 661: 654–663. DOI: https://doi.org/10.1016/j.scit....
 
22.
Moharana A., Mohanty S., Lenka D., Nayak R.K., Jena S.N., Satapathy M., Jyothi G.B.N. 2023. Effect of seed priming and coating with boron and molybdenum on seed physiological quality and seed yield of groundnut (Arachis hypogaea L.). The Pharma Innovation 12 (1): 1021–1026.
 
23.
Muhammad I., Kolla M., Volker R., Günter N. 2015. Impact of nutrient seed priming on germination, seedling development, nutritional status and grain yield of maize. Journal of Plant Nutrition 38 (12): 1803–1821. DOI: https://doi.org/10.1080/019041....
 
24.
Nečajeva J., Borodušķe A., Nikolajeva V., Seņkovs M., Kalniņa I., Roga A., Skinderskis E., Fridmanis D. 2023. Epiphytic and endophytic fungi colonizing seeds of two Poaceae weed species and Fusarium spp. seed degradation potential in vitro. Microorganisms 11 (1): 184. DOI: https://doi.org/10.3390/microo....
 
25.
Ni L., Punja Z.K. 2020. Effects of a foliar fertilizer containing boron on the development of Sclerotinia stem rot (Sclerotinia sclerotiorum) on canola (Brassica napus L.) leaves. Journal of Phytopathology 168: 47–55. DOI: https://doi.org/10.1111/jph.12....
 
26.
Pallaoro D.S., Camili E.C., Guimarães S.C., Albuquerque M.C.D.F. 2016. Methods for priming maize seeds. Journal of Seed Science 38: 148–154. DOI: https://doi.org/10.1590/2317-1....
 
27.
Picanço B.B.M., Ferreira S., Fontes B.A., Oliveira L.M., Silva B.N., Einhardt A.M., Rodrigues F.Á. 2021. Soybean resistance to Phakopsora pachyrhizi infection is barely potentiated by boron. Physiological and Molecular Plant Pathology 115: 101668. DOI: https://doi.org/10.1016/j.pmpp....
 
28.
Qin G., Zong Y., Chen Q., Hua D., Tian S. 2010. Inhibitory effect of boron against Botrytis cinerea on table grapes and its possible mechanisms of action. International Journal of Food Microbiology 138 (1–2): 145–150. DOI: https://doi.org/10.1016/j.ijfo....
 
29.
Rehman A., Farooq M., Cheema Z.A., Wahid A. 2013. Role of boron in leaf elongation and tillering dynamics in fine-grain aromatic rice. Journal of Plant Nutrition 36 (1): 42–54. DOI: https://doi.org/10.1080/019041....
 
30.
Rasool T., Ahmad R., Farooq M. 2019. Seed priming with micronutrients for improving the quality and yield of hybrid maize. Gesunde Pflanzen 71: 37–44. https://doi.org/10.1007/s10343....
 
31.
Sadeghi H., Khazaei F., Yari L., Sheidaei S. 2011. Effect of seed osmopriming on seed germination behavior and vigor of soybean (Glycine max L.). ARPN Journal of Agricultural and Biological Science 6 (1): 39–43.
 
32.
Salehzade H., Shishvan M.I., Ghiyasi M., Forouzin F., Siyahjani A.A. 2009. Effect of seed priming on germination and seedling growth of wheat (Triticum aestivum L.). Research Journal of Biological Sciences 4 (5): 629–631.
 
33.
Shahverdi M.A., Omidi H., Tabatabaei S.J. 2017. Determination of optimum duration and concentration of Stevia (Stevia rebaudiana Bert.) seed priming with boric acid (H3BO3). Turkish Journal of Agricultural Research 4 (1): 24–30. DOI: https://doi.org/10.19159/tutad....
 
34.
Sher A., Sarwar T., Nawaz A., Ijaz M., Sattar A., Ahmad S. 2019. Methods of seed priming. p. 1–10. In: “Priming and Pretreatment of Seeds and Seedlings. Implication in Plant Stress Tolerance and Enhancing Productivity in Crop Plants” (M. Hasanuzzaman, V. Fotopoulos, eds.). Springer Nature Singapore Pte. Ltd.
 
35.
Singh N.D., Sharma A.K., Dwivedi P., Patil R.D., Kumar M. 2007. Citrinin and endosulfan induced teratogenic effects in Wistar rats. Journal of Applied Toxicology 27: 143–151. DOI: https://doi.org/ 10.1002/jat.1185.
 
36.
Vankudoth K.R., Sivadeveuni G., Reddy S.M. 2015. Influence of different species of Penicillium and their culture filtrates on seed germination and seedling growth of sorghum. Journal of Biochemical Technology 5 (4): 832–837.
 
37.
Waqas M., Korres N.E., Khan M.D., Nizami A.S., Deeba F., Ali I., Hussain H. 2019. Advances in the concept and methods of seed priming. p. 11–41. In: “Priming and Pretreatment of Seeds and Seedlings. Implication in Plant Stress Tolerance and Enhancing Productivity in Crop Plants” (M. Hasanuzzaman, V. Fotopoulos, eds.). Springer Nature Singapore Pte. Ltd.
 
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