ORIGINAL ARTICLE
Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate
 
More details
Hide details
1
Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
 
2
Rice Research and Development Division, Rice Department, Bangkok 10900, Thailand
 
3
Chainat Rice Research Center, Chainat 17000, Thailand
 
 
Submission date: 2017-08-01
 
 
Acceptance date: 2017-11-06
 
 
Corresponding author
Teerada Wangsomboondee
Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
 
 
Journal of Plant Protection Research 2017;57(4):338-346
 
KEYWORDS
TOPICS
ABSTRACT
Beauveria bassiana is an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates of B. bassiana that can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates of B. bassiana obtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper, Nilaparvata lugens (Stål), of this mutant isolate was also prominent.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (30)
1.
Ansari M.A., Butt T.M. 2011. Effects of successive subculturing on stability, virulence, conidial yield, germination and shelf-life of entomopathogenic fungi. Journal of Applied Journal of Applied Microbiology 110 (6): 1460–1469. DOI: 10.1111/j.1365--2672.2011.04994.x.
 
2.
Avanti B., Balaraman K., Gopinath R. 2014. Development of higher temperature tolerant mutant of Beauveria bassiana and Verticillium lecanii. International Journal of Life Sciences Biotechnology and Pharma Research 3 (3): 109–112.
 
3.
Bugeme D.M., Maniania N.K., Knapp M., Boga H.I. 2008. Effect of temperature on virulence of Beauveria bassiana and Metarhizium anisopliae isolates to Tetranychus evansi. Experimental and Applied Acarology 46 (1–4): 275–285. DOI: 1007/s10493-008-9179-1
 
4.
Carneiro A.A., Gomes E.A., Guimarães C.T., Fernandes F.T., Carneiro N.P., Cruz I. 2008. Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. Pesquisa Agropecuária Brasileira 43 (4): 513–520. DOI:http://dx.doi.org/ 10.1590/S0100-204X2008000400010
 
5.
Feofilova E.P., Ivashechkin A.A., Alekhin A.I., Sergeeva Ya.E. 2012. Fungal spores: dormancy, germination, chemical composition, and role in biotechnology. Applied Biochemistry and Microbiology 48 (1): 1–11. DOI: 10.1134/S0003683812010048.
 
6.
Hallsworth J.E., Magan N. 1999. Water and temperature relations of growth of the entomogenous fungi Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces farinosus. Journal of Invertebrate Pathology 74 (3): 261–266. DOI:1006/jipa.1999.4883.
 
7.
Ho H.L., Ho K.F. 2015. Aspergillus brasiliensis for overproduction of xylanase in submerged fermentation through UV irradiation and chemicals mutagenesis. Journal of Advances in Biology & Biotechnology 3 (3): 117–131. DOI: 10.9734/JABB/2015/17274.
 
8.
Humber R.A. 2005. Entomopathogenic fungal identification. Available on: http://www.ars.usda.gov/ SP2UserFiles/Place/80620510/APSwkshoprev.pdf.
 
9.
Inglis G.D., Johnson D.L., Goettel M.S. 1997. Effects of temperature and sunlight on mycosis (Beauveria bassiana) (Hyphomycetes: Sympodulosporae) of grasshoppers under field conditions. Environmental Entomology 26 (2): 400–409. DOI: https://doi.org/10.1093/ee/26.....
 
10.
Johny S., Kyei-Poku G. 2014. A molecular tool for detection and tracking of a potential indigenous Beauveria bassiana strain for managing emerald ash borer populations in Canada. Journal of Invertebrate Pathology 122: 16–21.
 
11.
Krutmuang P. 2011. Brown planthopper (Nilaparvata lugens) and pest management in Thailand. In: Conference on International Research on Food Security, Natural Resource Management and Rural Development, University of Bonn, Germany, October 5–7, 2011. Available on: https://www.researchgate.net/p... [Accessed: August 12, 2015]
 
12.
Lawrence C.W., Christensen R.B. 1979. Ultraviolet-induced reversion of cycl alleles in radiation-sensitive strains of yeast III reu3 mutant strains. Genetics 92: 397–408.
 
13.
Leger R.J., Nelson J.O., Screen S.E. 1999. The entomopathogenic fungus Metarhizium anisopliae alters ambient pH, allowing extracellular protease production and activity. Microbiology 145 (1999): 2691–2699.
 
14.
Li M., Li S., Xu A., Lin H., Chen D., Wang H. 2014. Selection of Beauveria isolates pathogenic to adults of Nilaparvata lugens. Journal of Insect Science 14 (32): 1–12.
 
15.
Liu H., Zhao X., Guo M., Liu H., Zheng Z. 2015. Growth and metabolism of Beauveria bassiana spores and mycelia. BMC Microbiology 15: 1–12. DOI: 10.1186/s12866-015-0592-4.
 
16.
Mohd-Yusoff N.F., Ruperao P., Tomoyoshi N.E., Edwards D., Gresshoff P.M., Biswas B., Batley J. 2015. Scanning the effects of ethyl methanesulfonate on the whole genome of Lotus japonicus using second-generation sequencing analysis. G3 (Bethesda) 5 (4): 559–567. DOI: 10.1534/g3.114.014571.
 
17.
Momose H., Gregory K.F. 1998. Temperature-sensitive mutants of Saccharomyces cerevisiae variable in the methionine content of their protein. Applied and Environmental Microbiology 35 (4): 641–647.
 
18.
Orduño-Cruz N., Guzmán-Franco A.W., Rodríguez-Leyva E., Alatorre-Rosas R., González-Hernández H., Mora-Aguilera G., Rodríguez-Maciel, J.C. 2015. In vitro selection of a fungal pathogen for use against Diaphorina citri. Biological Control 90: 6–15. DOI: http://dx.doi.org/10.1016/j.bi....
 
19.
Qazzaz F.O., Al-Masri M.I., Barakat R.M. 2015. Effectiveness of Beauveria bassiana native isolates in the biological control of the Mediterranean fruit fly (Ceratitis capitata). Advances in Entomology 3: 44–55. DOI: http://dx.doi.org/10.4236/ae.2....
 
20.
Rehner S.A., Minnis A.M., Sung G., Luangsa-ard J.J., Devotto L., Humber R.A. 2011. Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103 (5): 1055–1073. DOI: 10.3852/10-302.
 
21.
Safavi S.A. 2010. Isolation, identification and pathogenicity assessment of a new isolate of entomopathogenic fungus, Beauveria bassiana in Iran. Journal of Plant Protection Research 50 (2): 158–163.
 
22.
Sega G.A. 1984. A review of the genetic effects of ethyl methanesulfonate. Mutation Research 134 (2–3): 113–142.
 
23.
Shimazu M. 2004. Effects of temperature on growth of Beauveria bassiana F-263, a strain highly virulent to the Japanese pine sawyer, Monochamus alternatus, especially tolerance to high temperatures. Applied Entomology and Zoology 39 (3): 469–475. DOI: http://doi.org/10.1303/aez.200....
 
24.
Shiwa Y., Fukushima-Tanaka S., Kasahara K., Horiuchi T., Yoshikawa H. 2012. Whole-genome profiling of a novel mutagenesis technique using proofreading-deficient DNA polymerase δ. International Journal of Evolutionary Biology 2012: 860797.
 
25.
Sivasankaran P., Eswaramoorthy S., David H. 1998. Influence of temperature and relative humidity on the growth, sporulation and pathogenicity of Beauveria bassiana. Journal of Biological Control 12: 71–75.
 
26.
Svedese V.M., Lima E.Á.L.A., Porto A.L.F. 2013. Horizontal transmission and effect of the temperature in pathogenicity of Beauveria bassiana against Diatraea saccharalis (Lepidoptera: Crambidae). Brazilian Archives of Biology and Technology 56 (3): 413–419. DOI: http://dx.doi.org/10.1590/S151....
 
27.
Tefera T., Pringle K. 2003. Germination, radial growth, and sporulation of Beauveria bassiana and Metarhizium anisopliae isolates and their virulence to Chilo partellus (Lepidoptera: Pyralidae) at different temperatures. Biocontrol Science and Technology 13 (7): 699–704. DOI: http://dx.doi.org/10.1080/0958....
 
28.
The Thai Meteorological Department, Annual Mean Temperature in Thailand. 2017. Available on: https://www.tmd.go.th/climate/... [Accessed: September 10, 2016].
 
29.
Wang G., Paredes-Sabja D., Sarker M.R., Green C., Setlow P., Li Y.Q. 2012. Effects of wet heat treatment on the germination of individual spores of Clostridium perfringens. Journal of Applied Microbiology 113 (4): 824836. DOI: 10.1111/j.1365-2672.2012.05387.x.
 
30.
Zimmermann G. 2007. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science and Technology 17 (6): 53–596. DOI: http://dx.doi.org/10.1080/0958....
 
eISSN:1899-007X
ISSN:1427-4345
Journals System - logo
Scroll to top