Effects of Artemisia annua methanolic extract on the enzymatic components of intermediary metabolism and the antioxidant system of Pseudococcus viburni Signoret
Samar Ramzi 1, A-D
Ali Seraji 1, F
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Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Lahijan, Iran
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
Plant Protection Department, Research Center for Agriculture and Natural Resources, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-01-09
Acceptance date: 2018-04-23
Online publication date: 2018-10-18
Corresponding author
Samar Ramzi   

Tea Research Center, Horticulture Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Sheikh Zahed Street, 41362536521 Lahijan, Iran
Journal of Plant Protection Research 2018;58(3):289-296
Toxicity and physiological alterations were determined in Pseudococcus viburni nymphs treated with Artemisia annua methanolic extract. The leaf dipping bioassay showed LC50 values of 0.287% and 0.194% 24 and 48 hours post-exposure. Activities of general esterases were significantly higher in the control nymphs than in those which had been treated except for the 48 h time interval using α-naphtyl acetate. The activity of glutathione S-transferase using CDNB (1-chloro-2,4-dinitrobenzene) in the control nymphs, was significantly higher than in the control at both time intervals while no significant difference was observed after 24 h in addition to the higher enzymatic activity in the treated nymphs after 48 h. All three aminotransferases were significantly more active in the control nymphs except for time intervals of 24 h for γ-glutamyl transferase and 48 h for alanine aminotransferase. Higher activities of lactate dehydrogenase, acid- and alkaline phosphatase were found in the control nymphs than in treated nymphs for all time intervals. Activities of the enzymes involved in the antioxidant system including catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase was increased in the treated nymphs compared to the control. Results of the current study demonstrated toxic effects of A. annua methanolic extract on P. viburni nymphs causing mortality and physiological turbulences.
The authors have declared that no conflict of interests exist.
Abbasipour H., Taghavi A. 2007. Description and seasonal abundance of the tea mealybug, Pseudococcus viburni (Affinis) (Signoret) (Homoptera: Pseudococcidae) Found on Tea in Iran. Journal of Entomology 4: 474−478. DOI: 10.3923/je.2007.474.478.
Addy S.K., Goodman R.N. 1972. Polyphenol oxidase and peroxidase activity in apple leaves inoculated with a virulent or an avirulent strain for Ervinia amylovora. Indian Phytopathology 25 (4): 575–579.
Asada K. 1984. Chloroplasts: formation of active oxygen and its scavenging. Methods in Enzymology 105: 422–429. DOI: https://doi.org/10.1016/S0076-....
Balinsky D., Bernstein R.E. 1963. The purification and properties of glucose-6-phosphate dehydrogenase from human erythrocytes. Biochimica Biophysica Acta 67: 313−315. DOI: https://doi.org/10.1016/0926-6....
Bessey O.A., Lowry O.H., Brock M.J. 1946. A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum. Journal of Biological Chemistry 164: 321–329.
Bhakuni R.S., Jain D.C., Sharma R.P., Kumar S. 2001. Secondary metabolites of Artemisia annua and their biological activity. Current Science 80 (1): 35–49.
Daane K.M., Cooper M.L., Triapitsyn S.V., Andrews Jr J.W., Ripa R. 2008. Parasitoids of obscure mealybug, Pseudococcus viburni (Hem.: Pseudococcidae) in California: establishment of Pseudaphycus flavidulus (Hym.: Encyrtidae) and discussion of related parasitoid species. Biocontrol Science and Technology 18 (1): 43–57. DOI: https://doi.org/10.1080/095831....
Dhivya K., Vengateswari G., Arunthirumeni M., Karthi S., Senthil-Nathan S., Shivakumar M.S. 2018. Bioprospecting of Prosopis juliflora (Sw.) DC seed pod extract effect on antioxidant and immune system of Spodoptera litura (Lepidoptera: Noctuidae). Physiological and Molecular Plant Pathology 101: 45−53. DOI: https://doi.org/10.1016/j.pmpp....
El-Hefny A.S., El-Sahn O.M.N., Yacoub S.S. 2011. Effect of some plant extracts on citrus mealy bug, Planococcus citri (Risso). Egyptian Journal of Agricultural Research 89 (2): 511−519.
Felton G.W., Summers C.B. 1995. Antioxidant systems in insects. Archives of Insect Biochemistry and Physiology 29 (2): 187–197.
Haghighian F., Sendi J.J., Aliakbar A.A., Javaherdashti M. 2008. The growth regulatory, deterrency and ovicidal activity of worm wood (Artemisia annua L.) on Tribolium confusum Duv. and identification of its chemical constituents by GC-MS. Pestycydy 1−2: 51−59.
Han Z., Moores G., Devonshire A., Denholm I. 1998. Association between biochemical marks and insecticide resistance in the cotton aphid, Aphis gossypii. Pesticide Biochemistry and Physiology 62 (3): 164–171. DOI: https://doi.org/10.1006/pest.1....
Hemingway J., Karunatne S.H.P.P. 1998. Mosquito carboxylesterases: A review of the molecular biology and biochemistry of a major insecticide resistance mechanism. Medical and Veterinary Entomology 12 (1): 1–12.
Hollingsworth R.G. 2005. Limonene, a citrus extract, for control of mealybugs and scale insects. Journal of Economic Entomology 98 (3): 772−779. DOI: https://doi.org/10.1603/0022-0....
Isman M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51: 45–66. DOI: 10.1146/annurev.ento.51.110104.151146.
King J. 1965. The dehydrogenases or oxidoreductases lactate dehydrogenase. p. 83–93. In: “Practical Clinical Enzymology” (King J., ed.). Van Nostrand, London, 363 pp.
Klowden M.J. 2012. Physiological Systems in Insects. 3th ed. Academic Press, New York, 682 pp.
LeOra Software. 1987. Polo-PC: a user g uide to probit or logit analysis. LeOra Software, Berkeley, CA.
Lothar T. 1998. Clinical Laboratory Diagnostics: Use and Assessment of Clinical Laboratory Results. TH-Books Verlagsgeselschaft, Frankfurt/Main, Germany, 1727 pp.
Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193 (1): 265–275.
Mafi S. 1997. Identification of mealybugs in Mazandaran province by considering the dominant species and natural enemies. MSc thesis. Tarbiat Modares University, Tehran, Iran, 112 pp.
Mardani-Talaee M., Zibaee A., Nouri-Ganbalani G., Razmjou J. 2016. Chemical and organic fertilizers affect physiological performance and antioxidant activities in Myzus persicae (Hemiptera: Aphididae). Invertebrate Survival Journal 13: 122−133.
McCord J.M., Fridovich I. 1969. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry 244 (22): 6049–6055.
Moharramipour S., Nazemi R.J., Talebi A.S., Fathipour Y. 2003. The effects of Nerium oleander, Lavandula officinalis and Ferula asafoetida extracts on feeding indices of adult Tribolium castaneum. Journal of Entomological Society of Iran 23: 69–90. (in Persian).
Mojarab-Mahboubkar M., Sendi J.J., Aliakbar. 2015. Effect of Artemisia annua L. essential oil on toxicity, enzyme activities, and energy reserves of cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Journal of Plant Protection Research 55 (4): 371−377. DOI: https://doi.org/10.1515/jppr-2....
Mojarab-Mahboubkar M., Sendi J.J. 2016. Chemical composition, insecticidal and physiological effect of methanol extract of sweet wormwood (Artemisia annua L.) on Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Toxin Reviews 35 (3−4): 106−115. DOI: https://doi.org/10.1080/155695....
Oppenoorth F., van der Pas L.J.T., Houx N.W.H. 1979. Glutathione S-transferase and hydrolytic activity in a tetrachlorvinphos-resistant strain of housefly and their influence on resistance. Pesticide Biochemistry and Physiology 11 (1−3): 176–188. DOI: https://doi.org/10.1016/0048-3....
Pardini R.S. 1995. Toxicity of oxygen from naturally occuring redox-active pro-oxidants. Archives of Insect Biochemistry and Physiology 29: 101–118. DOI: https://doi.org/10.1002/arch.9....
Piragalathan A., Pakeerathan K., Thirukkumaran G., Mikunthan G. 2014. Efficacy of different insecticides and bio-rationals against papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococidae) infestation in home gardens. Middle East Journal of Scientific Research 21 (10): 1689−1693. DOI: 10.5829/idosi.mejsr.2014.21.10.82293.
Prishanthini M., Vinobaba M. 2014. Efficacy of some selected botanical insecticides against the cotton mealybug, Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae). International Journal of Scientific and Research Publications 4 (3): 1−6.
Ramzi S., Seraji A., Azadi Gonbad R., Mirhaghparast S.K., Mojib Haghghadam Z., Haghighat S. 2018. Toxicity of Artemisia annua (Asteraceae) essential oil on the tea mealy bug, Pseudococcus viburni Sigornet (Hemiptera: Pseudococcidae). Archives of Phytopathology and Plant Protection 50 (19−20): 941−956. DOI: doi.org/10.1080/03235408.2017.1352223.
Rizvi S.A.H., Ikhlaq M.N., Jaffar S., Hussain S. 2015. Efficacy of some selected synthetic chemical insecticides and biopesticides against cotton mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) under agro ecological conditions of Peshawar, Pakistan. Journal of Entomology and Zoology Studies 3 (6): 223−226.
Senthil-Nathan S., Chung P.G., Murugan K. 2006. Combined effect of biopesticides on the digestive enzymatic profiles of Cnaphalocrocis medinalis (Guenée) (the rice leaffolder) (Insecta: Lepidoptera: Pyralidae). Ecotoxicology and Environmental Safety 64 (3): 382–389. DOI: https://doi.org/10.1016/j.ecoe....
Shekari M., Jalali Sendi J., Etenbari K., Zibaee A., Shadparvar A. 2008. Effects of Artemisia annua L. (Asteraceae) on nutritional physiology and enzyme activities of elm leaf beetle, Xanthogaleruca luteola Mull. (Coleoptera: Chrysomelidae). Pesticide Biochemistry and Physiology 91 (1): 66–74. DOI: https://doi.org/10.1016/j.pest....
Szasz G. 1976. Reaction-rate method for gamma-glutamyltransfe rase activity in serum. Clinical Chemistry 22 (12): 2051–2055.
Tate A.A., Meister A. 1985. Gamma-glutamyl transpeptidase from kidney. Methods in Enzymology 113: 400–419.
Thomas L. 1998. Clinical Laboratoty Diagnostic: Use and Assessment of Clinical Laboatory Results. 1st ed. TH-Bookks Verlagsgesellschaft, Frankfurt/Main, Germany, 1727 pp.
Wang Y., Oberley L.W., Murhammer D.W. 2001. Evidence of oxidative stress following the viral infection of two Lepidopteran insect cell lines. Free Radical Biological Medicine 31: 1448–1455. DOI: https://doi.org/10.1016/S0891-....
Zibaee A., Bandani A.R. 2010. A study on the toxicity of a medicinal plant, Artemisia annua L. (Asteraceae) extracts to the sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae). Journal of Plant Protection Research 50 (1): 79–85. DOI: 10.2478/v10045-010-0014-4.
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