• Detection of tomato leaf curl Palampur virus
  • Molecular characterization of the virus
  • Introduction of ToLCPlV into new regions
The complete genome sequence of tomato leaf curl Palampur virus (ToLCPlV), that infects a papaya (Carica papaya) plant, was determined. The virus genome was composed of 2,756 and 2,719 nucleotides (nt) in length, encoding all proteins required for replication, encapsidation and movement with the genome features typical of a bipartite begomovirus. Pairwise identity, derived using the Sequence Demarcation Tool (SDT), identified that the virus DNA A and DNA B shared maximum sequence identity 98–99% corresponding to the DNA A of ToLCPlV ([IR: Jir-T65X:08] JF501720) and 96–98% to the cognate partner DNA B of ToLCPlV ([IR: Jir1:T55P:07] FJ660423), respectively. The evolutionary relation using phylogenetic dendrograms of DNA A and DNA B genome components were clustered with ToLCPlV genomes of DNA A and DNA B of Iranian isolates. This study provides the first evidence of a bipartite ToLCPlV infecting papaya in the Sultanate of Oman and also indicates the requirement for more surveillance of this virus in Oman, as ToLCPlV is a major threat to tomato and other vegetable crops in South Asia (India and Pakistan) and in Iran.
The authors are grateful to SQU for providing funds under internal grant (IG/AGR/CROP/20/02) to MS Shahid.
grant (IG/AGR/CROP/20/02)
Julia Minicka
The authors have declared that no conflict of interests exist.
Ali I., Malik A.H., Mansoor S. 2010. First report of tomato leaf curl Palampur virus on bitter gourd in Pakistan. Plant Disease 94 (2): 276–276. DOI: 10.1094/PDIS-94-2-0276A.
Bananej K., Kraberger S., Varsani A. 2016. Okra enation leaf curl virus in papaya from Iran displaying severe leaf curl symptoms. Journal of Plant Pathology: 637–639. DOI: http://www.jstor.org/stable/44....
Bananej K., Shafiq M., Shahid M.S. 2021. Association of cotton leaf curl Gezira virus with tomato leaf curl betasatellite infecting Carica papaya in Iran. Australasian Plant Disease Notes 16: 1–4. DOI: https://doi.org/10.1007/s13314....
Brown J.K., Zerbini F.M., Navas-Castillo J., Moriones E., Ramos-Sobrinho R., Silva J.C., Fiallo-Olive E., Briddon R.W., Hernandez-Zepeda C., Idris A., Malathi V.G., Martin D.P., Rivera-Bustamante R., Ueda S., Varsani A. 2015. Revision of begomovirus taxonomy based on pairwise sequence comparisons. Archives of Virology 160 (6): 1593–619. DOI: 10.1007/s00705-015-2398-y.
Chang L.S., Lee Y.S., Su H.J., Hung T.H. 2003. First report of papaya leaf curl virus infecting papaya plants in Taiwan. Plant Disease 87: 204. DOI: https://doi.org/10.1094/PDIS-1....
Doyle J.J. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
Esmaeili M., Heydarnejad J., Massumi H., Varsani A. 2015. Analysis of watermelon chlorotic stunt virus and tomato leaf curl Palampur virus mixed and pseudo-recombination infections. Virus Genes 51 (3): 408–416. DOI: 10.1007/s11262-015-1250-5.
Hamim I., Borth W.B., Melzer M.J., Suzuki J.Y., Wall M.M., Hu J.S. 2019. Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization. Archives of Virology 164 (6): 1661–1665. DOI: 10.1007/s00705-019-04235-8.
Hanley-Bowdoin L., Settlage S.B., Orozco B.M., Nagar S., Robertson D. 1999. Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Critical Reviews in Plant Sciences 18 (1): 71–106. DOI: 10.1080/07352689991309162.
Heydarnejad J., Hesari M., Massumi H., Varsani A. 2013. Incidence and natural hosts of tomato leaf curl Palampur virus in Iran. Australasian Plant Pathology 42: 195–203. DOI: https://doi.org/10.1007/s13313....
Heydarnejad J., Mozaffari A., Massumi H., Fazeli R., Gray A.J., Meredith S., Lakay F., Shepherd D.N., Martin D.P., Varsani A. 2009. Complete sequences of tomato leaf curl Palampur virus isolates infecting cucurbits in Iran. Archives of Virology 154 (6): 1015–1018. DOI: 10.1007/s00705-009-0389-6.
Idris A.M., Shahid M.S., Briddon R.W., Khan A., Zhu J.K., Brown J.K. 2011. An unusual alphasatellite associated with monopartite begomoviruses attenuates symptoms and reduces betasatellite accumulation. Journal of General Virology 92 (3): 706–717. DOI: 10.1099/vir.0.025288-0.
Khan A., Akhtar S., Al-Shihi A., Al-Hinai F., Briddon R. 2012. Identification of cotton leaf curl Gezira virus in papaya in Oman. Plant Disease 96: 1704. DOI: 10.1094/PDIS-05-12-0438-PDN.
Martin D.P., Murrell B., Golden M., Khoosal A., Muhire B. 2015. RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evolution 1 (1): vev003. DOI: https://doi.org/10.1093/ve/vev....
Muhire B.M., Varsani A., Martin D.P. 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9: e108277. DOI: https://doi.org/10.1371/journa....
Namrata J., Saritha R.K., Datta D., Singh M., Dubey R.S., Rai A.B., Rai M. 2010. Molecular characterization of tomato leaf curl Palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (Cucurbita moschata) in India. Indian Journal of Virology 21 (2): 128–132. DOI: 10.1007/s13337-011-0022-7.
Nehra C., Marwal A., Verma R.K., Mishra M., Sharma P., Gaur R. 2019. Papaya yellow leaf curl virus: A newly identified begomovirus infecting Carica papaya L. from the Indian subcontinent. The Journal of Horticultural Science and Biotechnology 94: 475–480. DOI: https://doi.org/10.1080/146203....
Rojas M.R., Hagen C., Lucas W.J., Gilbertson R.L. 2005. Exploiting chinks in the plant’s armor: Evolution and emergence of geminiviruses. Annual Review of Phytopathology 43: 361–394. DOI: 10.1146/annurev.phyto.43.040204.135939.
Shafiq M., Sattar M.N., Shahid M.S., Al-Sadi A.M., Briddon R.W. 2021. Interaction of watermelon chlorotic stunt virus with satellites. Australasian Plant Pathology 50 (1): 117–128. DOI: https://doi.org/10.1007/s13313....
Shahid M.S., Yoshida S., Khatri-Chhetri G.B., Briddon R.W., Natsuaki K.T. 2013. Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal. Virus Genes 46: 581–584. DOI: 10.1007/s11262-013-0888-0.
Shen W., Tuo D., Yang Y., Yan P., Li X., Zhou P. 2014. First report of Ageratum yellow vein virus associated with a new betasatellite infecting Carica papaya in China. Journal of Plant Pathology 96 (3): 610. DOI: 10.4454/jpp.v1i1.3199.
Tang Y., He Z., She X., Yu L. 2018. First report of papaya leaf curl China virus infecting Acalypha australis in China. Plant Disease 102: 1674. DOI: https://doi.org/10.1094/PDIS-0....
Walker P.J., Siddell S.G., Lefkowitz E.J., Mushegian A.R., Adriaenssens E.M., Alfenas-Zerbini P., Davison A.J., Dempsey D.M., Dutilh B.E., García M.L., Harrach B., Harrison R.L., Hendrickson R.C., Junglen S., Knowles N.J., Krupovic M., Kuhn J.H., Lambert A.J., Łobocka M., Nibert M.L., Oksanen M., Orton R.J., Robertson D.L., Rubino L., Sabanadzovic S., Simmonds P., Smith D.B., Suzuki N., Van Dooerslaer K., Vandamme A.-M., Varsani A., Zerbini F.M. 2021. Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses. Archives of Virology 166: 2633–2648. DOI: 10.1007/s00705-021-05156-1.
Wyatt S., Brown J. 1996. Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86 (12): 1288–1293. DOI: https://doi.org/10.1094/Phyto-....
Journals System - logo
Scroll to top