ORIGINAL ARTICLE
Genetic identification of an invasive plant (Parthenium hysterophorus) and its crude extract phytotoxicity against palm oil weed (Diodia ocimifolia)
1
Department of Chemical Technology and Food, Politeknik Tun Syed Nasir Syed Ismail, Johor, Malaysia
2
Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
3
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
4
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-01-13
Acceptance date: 2022-03-30
Online publication date: 2022-06-29
Corresponding author
Norazlinaliza Salim
Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, UPM, 43400, Serdang, Malaysia
Integrated Chemical Biophysics Research, Faculty of Science, Universiti Putra Malaysia, UPM, 43400, Serdang, Malaysia
Journal of Plant Protection Research 2022;62(2):223-229
HIGHLIGHTS
- The local plant namely Rumput Miang Mexico was genetically identified as Parthenium hysterophorus by using DNA profiling technique.
- P.hysterophorus crude extract (PHCE) has significantly inhibited the germination of weed in Malaysian palm oil plantations.
- Overall, the seed germination showed an effect of hormesis where PHCE acted as a stimulant at a low dose, and inhibit as increasing the concentration.
KEYWORDS
TOPICS
ABSTRACT
This paper reports a study on genetic identification of Parthenium hysterophorus and the
ability of P. hysterophorus crude extract (PHCE) to inhibit germination of weeds in palm oil
plantations, particularly slender buttonweed (Diodia ocimifolia). Parthenium hysterophorus
is a noxious plant with invasive properties. It has become one of the world’s seven most
devastating and hazardous weed‑s due to its allelopathic properties. However, a comprehensive
study on its genetic identification and its herbicidal activity against weeds in palm
oil plantations had never been carried out. In the present study, internal transcribes spacer
(ITS) region-based analysis was used as a molecular
marker to ascertain the local Rumput
Miang Mexico (RMM) as a P. hysterophorus species. From the results, the DNA sequence
obtained was 99% matched with P. hysterophorus species database. In the dose-response
bioassay study, the phytotoxicity of PHCE on D. ocimifolia was simulated under laboratory
conditions. Methanolic extract of P. hysterophorus significantly inhibited germination and
growth of shoots and radicles of D. ocimifolia at low concentrations (1−5 g ∙ l−1). A study on
the herbicidal activity of PHCE could be an additional management approach for this phytotoxic
species and an alternative way to naturally control weeds on palm oil plantations.
ACKNOWLEDGEMENTS
The author would like to thank the Ministry of Higher
Education Malaysia for my Ph.D. scholarship for
Zainuddin N.J. and the Department of Agriculture
Malaysia for giving permission and assistance during
the collection of P. hysterophorus plant.
RESPONSIBLE EDITOR
Łukasz Sobiech
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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