ORIGINAL ARTICLE
Grain discoloration in different genotypes of durum wheat (Triticum durum L.) in Argentina:
associated mycobiota and peroxidase activity
Marina Sisterna
1,2
| 1 | Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Argentina |
| 2 | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), Argentina |
| 3 | Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Argentina |
| 4 | Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Argentina |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Maria Josefina Cipollone
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 118 s/n, 1900, La Plata, Argentina
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 118 s/n, 1900, La Plata, Argentina
Submission date: 2019-05-28
Acceptance date: 2019-08-07
Online publication date: 2020-03-06
Journal of Plant Protection Research 2020;60(1):14–20
KEYWORDS
TOPICS
ABSTRACT
Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases
grain yield, does not have a single etiology. It has been proposed that it is a consequence of
an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has
also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria
spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible
etiology of this disease in nine durum wheat genotypes from two localities of southern
Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain
discoloration) was recorded and mycobiota associated with this pathology was registered
following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to
genotypes of the locality that showed the highest incidence was measured.
The incidence among genotypes, localities and sowing dates varied, although the genotypes
with the higher and lower values of incidence were the same for all the variables
tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium
spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed
that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation
between controls either between genotypes with the lowest or the highest
incidence values. This suggests that peroxidase activity did not have a clear relationship
with grain discoloration. In this research, it is presumed that fungal infection is the main
cause of this disease.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (52)
1.
Acharya K., Dutta A.K., Pradhan P. 2011. ‘Bipolaris sorokiniana’ (Sacc.) Shoem.: The most destructive wheat fungal pathogen in the warmer areas. Australian Journal of Crop Science 5 (9): 1064.
2.
Bird E.T. 1975. A look at Australian durum wheats quality criteria and their assessment in the laboratory. p. 13–16. In: Proceedings of the 25th Annual Conference Research Australia. Chemistry Institute Cereal Chemistry Division, Newport, NSW.
3.
Cristea M.C., Delian E., Berca M. 2013. Particularities of the wheat varieties seeds germination under the black-point attack incidence. Romanian Biotechnological Letters 18 (4): 8441.
4.
Conner R.L., Davidson J.G.N. 1988. Resistance in wheat to black point caused by Alternaria alternata and Cochliobolus sativus. Canadian Journal of Plant Science 68 (2): 351–359. DOI: https://doi.org/10.4141/cjps88....
5.
Conner R.L., Kuzyk A.D. 1988. Black point incidence in soft white spring wheat in southern Alberta and Saskatchewan between 1982 and 1987. Canadian Plant Disease Survey 68: 27–31.
6.
Conner R.L., Hwang S.F., Stevens R.R. 1996. Fusarium proliferation: a new causal agent of black point in wheat. Canadian Journal of Plant Pathology 18 (4): 419–423.
7.
Dann E.K., Deverall B.J. 2000. Activation of systemic disease resistance in pea by an avirulent bacterium or a benzothiadiazole, but not by a fungal leaf spot pathogen. Plant Pathology 49 (3): 324–332. DOI: https://doi.org/10.1046/j.1365....
8.
Delwiche S., Miskelly D. 2017. Analysis of grain quality at receival. p. 513–570. In: “Cereal Grains. Assessing and Managing Quality Woodhead”. Publishing Series in Food Science, Technology and Nutrition. DOI: https://doi.org/10.1016/B978-0....
9.
Desjardins A.E., Busman M., Proctor R.H., Stessman R. 2007. Wheat kernel black point and fumonisin contamination by Fusarium proliferatum. Food additives and contaminants 24 (10): 1131–1137. DOI: 10.1080/02652030701513834.
10.
Dexter J.E., Matsuo R.R. 1982. Effect of smudge and blackpoint, mildewed kernels and ergot on durum wheat quality. Cereal Chemistry 59: 63–69.
11.
Draz I.S., El-Gremi S.M., Youssef W.A. 2016. Pathogens associated with wheat blackpoint disease and responsibility in pathogenesis. Journal of Environmental & Agricultural Sciences 8: 71–78.
12.
Fernandez M.R., Clarke J.M., DePauw R.M., Irvine R.B., Knox R.E. 1994. Black point and red smudge in irrigated durum wheat in southern Saskatchewan in 1990–1992. Canadian Journal of Plant Pathology 16 (3): 221–227. DOI: https://doi.org/10.1080/070606....
13.
Fernandez M.R., Clarke J.M., DePauw R.M., Irvine R.B., Knox R.E. 2000. Black point reaction of durum and common wheat cultivars grown under irrigation in southern Saskatchewan. Plant Disease 84 (8): 892–894. DOI: https://doi.org/10.1094/pdis.2....
14.
Fernandez M.R., DePauw R.M., Clarke J.M. 2001. Reaction of common and durum wheat cultivars to infection of kernels by Pyrenophora tritici-repentis. Canadian Journal of Plant Pathology 23 (2): 158–162. DOI: https://doi.org/10.1080/070606....
15.
Fernandez M.R., Conner R.L. 2011. Black point and smudge in wheat. Prairie Soils and Crops 4: 158–164.
16.
Fernandez M.R., Sissons M., Conner R.L., Wang H., Clarke J.M. 2011. Influence of biotic and abiotic factors on dark discoloration of durum wheat kernels. Crop science 51 (3): 1205–1214. DOI: 10.2135/cropsci2010.07.0433.
17.
Gargouri-Kammoun L., Bensassi F., Mnari-Hattab M., Rhouma A., Bacha H., Hajlaoui M.R. 2014. Identification of Alternaria species recovered from stored durum wheat kernels in Tunisia. Tunisian Journal of Plant Protection 9: 119–129.
18.
Greaney F.J., Wallace H.A.H. 1943. Varietal susceptibility to kernel smudge in wheat. Phytopathology 33: 4–5.
19.
Hadaway T.K., March T.J., Able J. 2003. The involvement of peroxidases in the formation of black point in barley. Conference in 11th Australian Barley Technicial Symposium and the 53rd Australian Cereal Chemistry Conference. 7–10 September 2013. Available on: http://www.proceedings.com.au/....
20.
Hudec K., Muchová D. 2008. Correlation between black point symptoms and fungal infestation and seedling viability of wheat kernels. Plant Protection Science 44 (4): 138–146. DOI: https://doi.org/10.17221/14/20....
21.
Khani M., Cheong J., Mrva K., Mares D. 2018. Wheat black point: Role of environment and genotype. Journal of Cereal Science. DOI: 10.1016/j.jcs.2018.04.012.
22.
Li Q.Y., Qin Z., Jiang Y.M., Shen C.C., Duan Z.B., Niu J.S. 2014. Screening wheat genotypes for resistance to black point and the effects of diseased kernels on seed germination. Journal of Plant Diseases and Protection 121 (2): 79–88. DOI: https://doi.org/10.1007/bf0335....
23.
Li Q.Y., Xu Q.Q., Jiang Y.M., Niu J.S., Xu K.G., He R.S. 2019. The correlation between wheat black point and agronomic traits in the North China Plain. Crop Protection 119: 17–23. DOI: https://doi.org/10.1016/j.crop....
24.
Lorenz K. 1986. Effects of black point on grain composition and baking quality of New Zealand. New Zealand Journal of Agricultural Research 29 (4): 711–718. DOI: https://doi.org/10.1080/002882....
25.
Madariaga R., Mellado M. 1988. Estudio sobre la enfermedad “punta negra” en trigos de primavera, sembrados en la zona centro-sur de Chile. [Studies on the “black point” disease in spring wheats, sowing in the central-southern zone of Chile]. Agricultura Técnica 48: 43–45. (in Spanish with English abstract).
26.
Manamgoda D.S., Rossman A.Y., Castlebury L.A., Crous P.W., Madrid H., Chukeatirote E., Hyde K.D. 2014. The genus Bipolaris. Studies in Mycology 79: 221–288. DOI: https://doi.org/10.1016/j.simy....
27.
March T.J., Able J.A., Schultz C.J., Able A.J. 2007. A novel late embryogenesis abundant protein and peroxidase associated with black point in barley grains. Proteomics 7 (20): 3800–3808. DOI: 10.1002/pmic.200700456.
28.
Marchionatto J.B. 1934. Enfermedades del trigo pocos conocidas y radicadas en la región oeste de la zona triguera. [Wheat diseases few known and located in the western region of the wheat zone production]. Boletín del Ministerio de Agricultura de la Nación 36: 293–299. (in Spanish).
29.
Mathur S.B., Cunfer B. 1993. Black Point. p. 13–21. In: “Seed-borne Diseases and Seed Health Testing of Wheat” (Mathur S.B., Cunfer B., eds.). Danish Government Institute of Seed Pathology for Developing Countries, Copenhagen, Denmark.
30.
Miravalles M., Beaufort V., Möckel F. 2008. Susceptibilidad relativa a escudete negro en variedades de trigo para fideos de Argentina. [Relative susceptibility to black point in durum wheat varieties in Argentina]. Phyton (Buenos Aires) 77: 263–273. (in Spanish with English abstract).
31.
Moschini R.C., Sisterna M.N., Carmona M.A. 2006. Modelling of wheat black point incidence based on meteorological variables in the southern Argentinean Pampas region. Australian Journal of Agricultural Research 57 (11): 1151–1156. DOI: https://doi.org/10.1071/ar0527....
32.
Nelson P.E., Dignani M.C., Anaissie E.J. 1994. Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews 7 (4): 479–504. DOI: 10.1128/CMR.7.4.479.
33.
Ostry V. 2008. Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin Journal 1 (2): 175–188. DOI: https://doi.org/10.3920/wmj200....
34.
Özer N. 2005. Determination of the fungi responsible for black point in bread wheat and effects of the disease on emergence and seedling vigour. Trakya University Journal of Science 6: 35–40.
35.
Patel D., Minipara D. 2016. Assessment of fungi associated with black point disease of wheat and genetic variation amongst the most dominantly isolated fungus [Alternaria alternata (FR.) KESSL.]. Assessment 11 (4): 2105–2110.
36.
Rotem J. 1994. The Genus Alternaria: Biology, Epidemiology, and Pathogenicity. 2nd ed. The American Phytopathological Society, St. Paul, USA, 326 pp.
37.
Scandalios J.G. 2005. Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Brazilian Journal of Medical and Biological Research 38 (7): 995–1014. DOI: https://doi.org/10.1590/s0100-....
38.
Sivanesan A. 1987. Graminicolous Species of Bipolaris, Curvularia, Drechslera, Exserohilum and their Teleomorphs. 1st ed. Mycological Papers, No. 158, CAB International, Wallingford, UK, 261 pp.
39.
Sisterna M.N., Sarandón S.J. 2000. Blackpoint incidence on durum wheat in Argentina: Influence of cultivar and location. Acta Agronómica Hungarica 48 (4): 395–401. DOI: https://doi.org/10.1556/aagr.4....
40.
Sisterna M.N., Sarandón S.J. 2005. Preliminary studies on the natural incidence of wheat black point under different nitrogen fertilization levels and tillage systems in Argentina. Plant Pathology Journal 4 (1): 26–28. DOI: https://doi.org/10.3923/ppj.20....
41.
Sisterna M.N., Sarandón S.J. 2010. Wheat Grain Discoloration in Argentina: Current Status. p. 54–64. In: “Plant science and biotechnology in South America: Focus on Argentina I. The Americas Journal of Plant Science and Biotechnology” (A. Di Benedetto, ed.). Global Science Books, Japan, 116 pp.
42.
Solanki V.A., Augustine N., Patel A.A. 2006. Impact of black point on wheat trade and its management. Indian Phytopathology 59: 44–47.
43.
Southwell R.J., Wong P.T.W., Brown J.F. 1980. Resistance of durum wheat cultivars to black point caused by Alternaria alternata. Australian Journal of Agricultural Research 31 (6): 1097–1101. DOI: https://doi.org/10.1071/ar9801....
44.
Sulman A., Fox G., Osman A., Inkerman A., Williamson P., Michalowitz M. 2001. Relationship between total peroxidase activity and susceptibility to black point in mature grain of some barley cultivars. Proceedings of the 10th Australian Barley Technical Symposium, Camberra, Australia.
45.
Toklu F., Akgül D.S., Biçici M., Karaköy T. 2008. The relationship between black point and fungi species and effects of black point on seed germination properties in bread wheat. Turkish Journal of Agriculture and Forestry 32: 267–272.
46.
Uarrota V.G., Moresco R., Schmidt E.C., Bouzon Z.L., da Costa Nunes E., de Oliveira Neubert E., Martins Peruch L.A., Rocha M., Maraschin M. 2016. The role of ascorbate peroxidase, guaiacol peroxidase, and polysaccharides in cassava (Manihot esculenta Crantz) roots under postharvest physiological deterioration. Food Chemistry 197: 737–746. DOI: 10.1016/j.foodchem.2015.11.025.
47.
Walker K.R., Able J.A., Mather D.E., Able A.J. 2008. Black point formation in barley: environmental influences and quantitative trait loci. Australian Journal of Agricultural Research 59 (11): 1021–1029. DOI: https://doi.org/10.1071/ar0807....
48.
Wang H., Fernandez M.R., McCaig T.N., Gan Y.T., DePauw R.M., Clarke J.M. 2003. Kernel discoloration and downgrading in spring wheat varieties in western Canada. Canadian Journal of Plant Pathology 25 (4): 350–361. DOI: https://doi.org/10.1080/070606....
49.
Warham F.J., Butler L.D., Sutton B.C. 1999. Seed Testing of Maize and Wheat. A Laboratory Guide, CIMMYT-CAB, 84 pp.
50.
Wiese M.V. 1987. Compendium of Wheat Diseases. American Phytopathological Society, St. Paul, MN, 112 pp.
51.
Williamson P.M. 1997. Black point of wheat: in vitro production of symptoms, enzymes involved and association with Alternaria alternata. Australian Journal of Agricultural Research 48 (1): 13–19. DOI: https://doi.org/10.1071/a96068.
52.
Xu K.G., Jiang Y.M., Li Y.K., Xu Q.Q., Niu J.S., Zhu X.X., Li Q.Y. 2018. Identification and pathogenicity of fungal pathogens causing black point in wheat on the North China plain. Indian Journal of Microbiology 58 (2): 1–6. DOI: https://doi.org/10.1007/s12088....
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