ORIGINAL ARTICLE
Grain discoloration in different genotypes of durum wheat (Triticum durum L.) in Argentina:
associated mycobiota and peroxidase activity
Marina Sisterna
1,2
1
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Argentina
2
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), Argentina
3
Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Argentina
4
Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Argentina
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2019-05-28
Acceptance date: 2019-08-07
Online publication date: 2020-03-06
Corresponding author
Maria Josefina Cipollone
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 118 s/n, 1900, La Plata, Argentina
Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 118 s/n, 1900, La Plata, Argentina
Journal of Plant Protection Research 2020;60(1):14–20
KEYWORDS
TOPICS
ABSTRACT
Wheat grain discoloration, a worldwide disease that lowers grain quality and decreases
grain yield, does not have a single etiology. It has been proposed that it is a consequence of
an abiotic mechanism, a response to environmental conditions or enzymatic activity. It has
also been suggest that it is a biotic mechanism, a fungal infection principally by Alternaria
spp. and Bipolaris sorokiniana. The present work was carried out to analyze the possible
etiology of this disease in nine durum wheat genotypes from two localities of southern
Buenos Aires province (Argentina) on two sowing dates. Incidence (percentage of grain
discoloration) was recorded and mycobiota associated with this pathology was registered
following ISTA rules. Peroxidase activity in an extract obtained from grains belonging to
genotypes of the locality that showed the highest incidence was measured.
The incidence among genotypes, localities and sowing dates varied, although the genotypes
with the higher and lower values of incidence were the same for all the variables
tested. The fungus Alternaria spp. was isolated the most frequently followed by Fusarium
spp., while Bipolaris sorokiniana was found the least frequently. Peroxidase activity showed
that all the treatments had similar levels of enzymatic activity, but there was no clear differentiation
between controls either between genotypes with the lowest or the highest
incidence values. This suggests that peroxidase activity did not have a clear relationship
with grain discoloration. In this research, it is presumed that fungal infection is the main
cause of this disease.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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