ORIGINAL ARTICLE
Higher induction of defense enzymes and cell wall reinforcement in maize by root associated bacteria for better protection against Aspergillus niger
1
Department of Biotechnology, Natubhai V. Patel College of Pure and Applied Sciences, Anand, India
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2019-01-11
Acceptance date: 2019-08-22
Online publication date: 2019-10-07
Corresponding author
Yachana Jha
Department of Biotechnology, Natubhai V. Patel College of Pure and Applied Sciences, Anand, India
Department of Biotechnology, Natubhai V. Patel College of Pure and Applied Sciences, Anand, India
Journal of Plant Protection Research 2019;59(3):341–349
KEYWORDS
Aspergillus nigerβ-13-glucanasebiotic factorphenylalanine ammonia lyaseRAPD analysisrhizosphereroot associated bacteria
TOPICS
ABSTRACT
Root associated bacteria were isolated from Suaeda nudiflora and two isolates were selected
for this study: rhizospheric Bacillus megaterium and endophytic Pseudomonas aeruginosa.
These isolates were inoculated into maize variety Narmada Moti during its germination.
TTC (2, 3, 5-triphenyl tetrazolium chloride) staining was used to confirm the association
of the isolates with the maize root. The effects of these root associated bacteria were tested
alone and in combinations for cell wall reinforcement and the induction of defense enzymes
such as phenylalanine ammonia lyase (PAL) and β-1,3-glucanase in the presence
of fungal pathogen Aspergillus niger in maize. The results indicated that the rhizospheric
bacteria had a greater fight response to fungal infection than the endophhytic bacteria due
to cell wall lignification as well as the rapid induction of higher concentrations of defense
related enzymes.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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