ORIGINAL ARTICLE
Characterization of the phenylalanine ammonia lyase gene from the rubber tree (Hevea brasiliensis Müll. Arg.) and differential response during Rigidoporus microporus infection
1
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand
2
Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand
Submission date: 2016-06-17
Acceptance date: 2016-10-28
Corresponding author
Korakot Nakkanong
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand
Journal of Plant Protection Research 2016;56(4):380–388
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KEYWORDS
TOPICS
ABSTRACT
Phenylalanine ammonia lyase (PAL) is a specific branch point enzyme of primary and secondary metabolism. It plays a key role in plant development and defense mechanisms. Phenylalanine ammonia lyase from Hevea brasiliensis (HbPAL) presented a complete open reading frame (ORF) of 2,145 bp with 721 encoded amino acids. The sequence alignment indicated that the amino acid sequence of HbPAL shared a high identity with PA L genes found in other plants. Phylogenetic tree analysis indicated that
HbPAL was more closely related to PALs in Manihot esculenta and Jatropha curcas
than to those from other plants. Transcription pattern analysis indicated that HbPAL
was constitutively expressed in all tissues examined, most highly in young leaves. The
HbPAL gene was evaluated by quantitative real-time PCR (qRT-PCR) after infection with
Rigidoporus microporus at 0, 12, 24, 48, 72 and 96 hours post inoculation. The expression patterns of the PA L gene differed among the three rubber clones used in the study. The transcription level of the white root rot disease tolerant clone, PB5/51 increased sharply during the latter stages of infection, while it was relatively subdued
in the white root rot disease susceptible clones, RRIM600 and BPM24. These results suggest that the HbPAL gene may play a role in the molecular defense response of H. brasiliensis to pathogen attack and could be used as a selection criterion for disease tolerance.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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