ORIGINAL ARTICLE
Evaluation of banana cultivars and the pathogenesis-related class 3 and 10 proteins in defense against Ralstonia syzygii subsp. celebesensis, the causal agent of banana blood disease
1
Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand
2
Department of Biology, Mahasarakham University, Faculty of Science, Maha Sarakham, Thailand
3
Department of Horticulture, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom, Thailand
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-04-27
Acceptance date: 2023-07-25
Online publication date: 2023-09-04
Corresponding author
Praphat Kawicha
Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand
Plant Genome and Disease Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand
Journal of Plant Protection Research 2023;63(3):375-386
HIGHLIGHTS
- Assessment of resistance in cultivated and wild bananas to Banana Blood Disease.
- The cultivar 'Khai Kasetsart 2' from the Musa (AA group) resisted the disease.
- Pathogenesis-related (PR) protein gene expression, specifically PR3 and PR10, was significantly increased in the resistant cultivar.
KEYWORDS
TOPICS
ABSTRACT
Banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc), is a major
threat to banana production in Southeast Asia. This study aimed to assess the resistance
of cultivated and wild banana accessions to Rsc and investigate the expression of pathogenesis-
related (PR) protein genes, namely PR3 and PR10, in disease-resistant bananas.
Bacterial isolates were isolated from infected bananas in Yala Province, Thailand, and their
pathogenicity and phylotype were confirmed, along with Rsc-specific PCR. Rsc-resistance
banana screening was conducted on 16 banana accessions, including cultivated and wild
types, using representative Rsc isolates. ‘Khai Kasetsart 2’ exhibited resistance (R), followed
by ‘Raksa’ with moderate resistance (MR). The expression of PR3 and PR10 genes was analyzed
in the resistant ‘Khai Kasetsart 2’ and susceptible ‘Hin’ bananas, revealing distinct expression
patterns. PR3 showed rapid upregulation on day 1 after inoculation (DAI), while
PR10 exhibited sustained upregulation from 1 to 7 DAI in the resistant cultivar. These findings
indicate the involvement of PR proteins in the defense response against Rsc and hold
promise for future breeding and disease management strategies in bananas.
ACKNOWLEDGEMENTS
The authors would like to express their gratitude to the Faculty
of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, for their support in facilitating this project.
Special thanks are extended to Dr. Araya Arjcharoen
Theanhom and Monthar Wongmaneeroj for providing
the clean cultures of banana cultivars.
FUNDING
This research was funded by Kasetsart University
through the Graduate School Fellowship Program and
the Agricultural Research Development Agency (Public
Organization) of Thailand or “ARDA”.
RESPONSIBLE EDITOR
Joanna Puławska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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