• This study carried out for estimation of polyphenols (TP) and induction of oxidative enzymes polyphenol oxidase (PPO) and peroxidase (POD) in the sunflower plants through seeds immersion in agrochemicals of salicylic acid (SA) and chitosan (CH) in addition to conidial suspension of T. harzianum and thereafter analysis plants content of carbohydrates and protein.
This study was carried out for the estimation of polyphenols (TP) and induction of oxidative enzymes polyphenol oxidase (PPO) and peroxidase (POD) in sunflower plants through seed immersion in agrochemicals of salicylic acid (SA) and water soluble chitosan (CH) in addition to a conidial suspension of Trichoderma harzianum and then analysis of plant content of carbohydrates and protein. The highest level of PPO 253.3 U ꞏ min–1 was detected in 50 ppm SA for 6 h. Next was T. harzianum when catalyzed PPO with 193.67 U ꞏ min–1. Peroxidase was substantially catalyzed in accordance with the increment of inducers. Sunflower roots induced TP with up to 4.88 mg . g–1 in plants treated with SA at 50 ppm for 6 h and then declined with an increasing SA dose. The total carbohydrate content in leaves of 320 mg ꞏ 100 g–1 was found in treatments of CH at 50 ppm for 6 h. In roots, a carbohydrate content of 500 mg ꞏ 100 g–1 was observed using CH 75 ppm for 6 h. Trichoderma harzianum remarkably increased proteins in leaves and roots by up to 25% compared to 16.9% in the control. These results suggest that inducing the plants’ own defense mechanism by applying salicylic acid and chitosan and bio-control of T. harzianum may offer alternative methods for controlling charcoal rot of sunflower due to the creation of defensive enzymes and could support plant vigor by enhancement of its protein and carbohydrate content.
Natasza Borodynko-Filas
The authors have declared that no conflict of interests exist.
Abbas H.K., Bellaloui N., Butler A.M., Nelson J.L., Abou-Karam M., Shier W.T. 2020. Phytotoxic responses of soybean (Glycine max L.) to botryodiplodin, a toxin produced by the charcoal rot disease fungus, Macrophomina phaseolina. Toxins (Basel) 12: 25. DOI: 10.3390/toxins12010025.
Abdel-Mawgoud A.M.R., Tantawy A.S., El-Nemr M., Sassine Y.N. 2010. Growth and yield responses of strawberry plants to chitosan application. European Journal of Scientific Research 39 (1): 170–177.
Ahmed G.A. 2016. Efficiency of some antioxidants and bioagents in controlling Rhizoctonia damping-off of snap bean. Middle East Journal of Applied Sciences 6: 748–758.
Amira R.D., Roshanida A.R., Rosli M.I., Zahrah M.S.F., Anuar A.M., Adha C.N. 2011. Bioconversion of empty fruit bunches (EFB) and palm oil mill effluent (POME) into compost using Trichoderma virens. African Journal of Biotechnology 10 (8): 18775–18780. DOI: 10.5897/AJB11.2751.
Angel L.P.L., Yusof M.T., Ismail I.S., Ping B.T.Y., Azni I.N.A.M., Kamarudin N.H., Sundram S. 2016. An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense. Journal of Microbiology 54: 732–744. DOI: 10.1007/s12275-016-6304-4.
Arfan M., Athar H.R., Ashraf M. 2007. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? Journal of Plant Physiology 164 (6): 685–694. DOI: 10.1016/j.jplph.2006.05.010.
Bakhoum G.Sh., Sadak M.Sh., Badr El. Abd 2020. Mitigation of adverse effects of salinity stress on sunflower plant (Helianthus annuus L.) by exogenous application of chitosan. Bulletin of the National Research Centre 44. DOI: https://doi.org/10.1186/s42269....
Bandara A.Y., Weerasooriya D.K., Bradley C.A., Allen T.W., Esker P.D. 2020. Dissecting the economic impact of soybean diseases in the United States over two decades. PLoS One 15 (4): e0231141. DOI: https://doi.org/10.1371/journa....
Bui F.Q., Almeida-da Silva C.L.C., Huynh B., Trinh A., Woodward J., Asadi H., Ojcius D.M. 2019. Association between periodontal pathogens and systemic disease. Biomedical Journal 42 (1): 27–35. DOI: 10.1016/j.bj.2018.12.001.
Caruso C., Chilosi G., Leonardi L., Bertini L., Margo P., Buonocore V., Caporale C. 2001. A basic peroxidase from wheat kernel with antifungal activity. Phytochemistry Journal 58 (5): 743–750. DOI: 10.1016/s0031-9422(01)00226-6.
Chavan S.S. 2007. Studies on fungal diseases of patchouli with special reference to wilt caused by Fusarium solani (Mart.) Sacc. Ph.D., University of Agricultural Sciences, Dharwad, India.
Chen Y.F., Zhan Y., Zhao X.M., Guo P., An H.L., Du Y.G. 2009. Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco. Plant Physiology and Biochemistry 47 (8): 724–731. DOI: 10.1016/j.plaphy.2009.03.009.
Cho M.H., No H.K., Prinyawiwatkul W. 2008. Chitosan treatments affect growth and selected quality of sunflower sprouts. Journal of Food Science 73 (1): S70–S77. DOI: 10.1111/j.1750-3841.2007.00607.x.
Doley K., Jite P. 2013. Disease management and biochemical changes in groundnut inoculated with Glomus fasciculatum and pathogenic Macrophomina phaseolina (Tassi) Goid. Journal Frontiers in Plant Science 3 (2): 21–26. DOI: http://reprints.icrisat.ac.in/....
Edmunds L.K. 1964. Combined relation of plant maturity texture and soil moisture to charcoal stalk rot development in grain sorghum. Phytopathology 54: 514–517.
El-Hai K.M.A., El-Metwally M., El-Baz S.M., Zeid A.M. 2009. The use of antioxidants and microelements for controlling damping-off caused by Rhizoctonia solani and charcoal rot caused by Macrophomina phaseolina on sunflower. Plant Pathology Journal 8 (3): 79–89. DOI: 10.3923/ppj.2009.79.89.
Gaddeyya G., Niharika P.S., Bharathi P., Kumar P.K.R. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. Advances in Applied Science Research 3 (4): 2020–2026.
Golubenko Z., Khashimova N., Beresneva Y., Ibragimov F. 2006. Influence of chitin-specific peroxidase isozymes on resistance of plants in the family Malvaceae to phytopathogens. p. 45. In: Proceedings of Peroxidase, July 7–9, 2006, Aveiro, Portugal.
González L.M., Guerrero Y.R., Rodríguez A.F., Vázquez M.N. 2015. Effect of seed treatment with chitosan on the growth of rice (Oryza sativa L.) seedlings cv. INCA LP-5 in saline medium. Journal of the Indian National Cartographic Association (INC) 36 (1): 136–142.
Govindappa M., Lokesh S., Ravishankar Rai V., Rudra Naik V., Raju S.G 2010. Induction of systemic resistance and management of safflower Macrophomina phaseolina root-rot disease by biocontrol agents. Archives of Phytopathology and Plant Protection 43 (1): 26–40. DOI: https://doi.org/10.1080/032354....
Gozzo F., Faoro F. 2013. Systemic acquired resistance (50 years after discovery): moving from the lab to the field. Journal of Agricultural and Food Chemistry 61 (51): 12473–12491. DOI: 10.1021/jf404156x.
Guan Y.-J., Hu J., Wang X.-J., Shao C.-X. 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B 10 (6): 427–433.
Gupta G.K., Sharma S.K., Ramteke R. 2012. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). Journal of Phytopathology 160: 167–180. DOI: https://doi.org/10.1111/j.1439....
Harman G.E. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96 (2): 190–194. DOI: 10.1094/PHYTO-96-0190.
Harman G.E., Doni F., Khadka R.B., Uphoff N. 2019. Endophytic strains of Trichoderma increase plants’ photosynthetic capability. Journal of Applied Microbiology 130 (2): 529–546. DOI: 10.1111/jam.14368.
Hidangmayum A., Dwivedi P. 2018. Plant responses to Trichoderma spp. and their tolerance to abiotic stresses: A review. Journal of Pharmacognosy and Phytochemistry 7 (1): 758–766.
Hosseini S.M., Hasanloo T., Mohammadi S. 2014. Physiological characteristics, antioxidant enzyme activities, and gene expression in 2 spring canola (Brassica napus L.) cultivars under drought stress conditions. Turkish Journal of Agriculture and Forestry 39: 413–420. DOI: 10.3906/tar-1405-102.
Inayati A., Sulistyowati L., Aini L.Q., Yusnawan E. 2020. Trichoderma virens-Tv4 enhances growth promoter and plant defense-related enzymes of mungbean (Vigna radiata) against soil-borne pathogen Rhizoctonia solani. Biodiversitas Journal of Biological Diversity 21 (6): 2410–2419. DOI: https://doi.org/10.13057/biodi....
Jain R., Rao B., Tare A.B. 2017. Comparative analysis of the spectrophotometry based total phenolic acid estimation methods. Journal of Analytical Chemistry 72 (9): 972–976. DOI: 10.1134/S106193481709009X.
Javaid A., Saddique A. 2012. Control of charcoal rot fungus Macrophomina phaseolina by extracts of Datura metel. Natural Product Research 26 (18): 1715–1720. DOI: 10.1080/14786419.2011.605363.
Karmel Reetha A., Lalitha Pavani S., Mohan S. 2014. Ecofriendly management of fungal antagonistic Trichoderma sp. against charcoal rot of sunflower caused by Macrophomina phaseolina (Tassi) Goid. Journal of Biopesticides 7 (1): 73–76.
Katiyar D., Hemantaranjan A., Singh B. 2015. Chitosan as a promising natural compound to enhance potential physiological responses in plant: a review. Indian Journal of Plant Physiology 20 (1): 1–9. DOI: 10.1007/s40502-015-0139-6.
Kavitha R., Umesha S. 2008. Regulation of defense-related enzymes associated with bacterial spot resistance in tomato. Phytoparasitica 36 (2): 144–159. DOI: https://doi.org/10.1007/BF0298....
Khaledi N., Taheri P. 2016. Biocontrol mechanisms of Trichoderma harzianum against soybean charcoal rot caused by Macrophomina phaseolina. Journal of Plant Protection Research 56 (1): 21–31. DOI: 10.1515/jppr-2016-0004.
Kumari N., Sharma I., Alam A., Sharma V. 2015. Screening of sorghum genotypes and biochemical changes for resistance to damage by Macrophomina phaseolina. Archives of Phytopathology and Plant Protection 9 (12): 760–775. DOI: https://doi.org/10.1080/032354....
Li L., Steffens J.C. 2002. Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 2: 239–247. DOI: 10.1007/s00425-002-0750-4.
Li S.J., Zhu T.H. 2013. Biochemical response and induced resistance against anthracnose (Colletotrichum camelliae) of camellia (Camellia pitardii) by chitosan oligosaccharide application. Forest Pathology 43 (1): 67–76. DOI: https://doi.org/10.1111/j.1439....
Llorens E., García-Agustín P., Lapeña L. 2017. Advances in induced resistance by natural compounds: towards new options for woody crop protection. Scientia Agricola 74 (1): 90–100. DOI: https://doi.org/10.1590/1678-9....
Lodha S., Mawar R. 2020. Population dynamics of Macrophomina phaseolina in relation to disease management: A review. Journal of Phytopathology 168: 1–17. DOI: https://doi.org/10.1111/jph.12....
Mahdavi B., Sanavy S.A.M.M., Aghaalikhani M., Sharifi M., Dolatabadian A. 2011. Chitosan improves osmotic potential tolerance in safflower (Carthamus tinctorius L.) seedlings. Journal of Crop Improvement 25 (6): 728–741.
Małolepsza U., Nawrocka J., Szczech M. 2017. Trichoderma virens 106 inoculation stimulates defence enzyme activities and enhances phenolic levels in tomato plants leading to lowered Rhizoctonia solani infection. Biocontrol Science and Technology 27 (2): 180–199. DOI: https://doi.org/10.1080/095831....
Marquez N., María L.G., Stéphane D., Daniel A.D. 2021. Macrophomina phaseolina: general characteristics of pathogenicity and methods of control. Frontiers in Plant Science 12: 634397. DOI: 10.3389/fpls.2021.634397.
Muftugil N. 1985. The peroxidase enzyme activity of some vegetables and its resistance to heat. Journal of the Science Food and Agriculture 36: 877–880. DOI: https://doi.org/10.1002/jsfa.2....
Mydlarz L.D., Harvell C.D. 2006. Peroxidase activity and inducibility in the sea fan coral exposed to a fungal pathogen. Comparative Biochemistry and Physiology 146 (1): 54–62. DOI: 10.1016/j.cbpa.2006.09.005.
Ojha S., Chatterjee N.Ch. 2012. Induction of resistance in tomato plants against Fusarium oxysporum f. sp. lycopersici mediated through salicylic acid and Trichoderma harzianum. Journal of Plant Protection Research 52 (2): 220–225. DOI: 10.2478/v10045-012-0034-3.
Pieterse M.J., Zamioudis C., Berendsen R.L., Weller D.M., Van Wees S.C.M., Bakker P.A. 2014. Induced systemic resistance by beneficial microbes. Annual Review of Phytopathology 52: 347–375. DOI: 10.1146/annurev-phyto-082712-102340.
Pitotti A., Elizalde B.E., Anese M. 1994. Effect of caramelization and Maillard reaction products on peroxide activity. Journal of Food Biochemistry 18: 445–457. DOI: https://doi.org/10.1111/j.1745....
Reddy P.L.N., Babu B.S., Radhaiah A., Sreeramulu A. 2014. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor district, India. International Journal of Current Microbiology and Applied Sciences 3 (7): 761–771.
Regnier T., Macheix J. 1996. Changes in wall-bound phenolic acids, phenylalanine and tyrosine ammonia-lyases and peroxidases in developing durum wheat grains (Triticum turgidum L. var. durum). Journal of Agricultural Food Chemistry 44 (7): 1727–1730. DOI: https://doi.org/10.1021/jf9506....
Salvatore M.M., Félix C., Lima F., Ferreira V., Naviglio D., Salvatore F. 2020. Secondary metabolites produced by Macrophomina phaseolina isolated from Eucalyptus globulus. Agriculture 10: 72. DOI: https://doi.org/10.3390/agricu....
Sharma B.L., Singh S.P., Sharma M.L. 2012. Bio-degradation of crop residues by Trichoderma species vis-à vis nutrient quality of the prepared compost. Sugar Tech 14(2): 174–180. DOI: 10.1007/s12355-011-0125-x.
Sharma A. 2011. Effect of Macrophomina phaseolina infection in Sesamum. Progressive Agricultural 11: 242–246.
Shi C., Dai Y., Xu X., Xie Y., Liu Q. 2002. The purification of polyphenol oxidase from tobacco. National Center for Biotechnology Information Journal 24 (1): 51–55. DOI: 10.1006/prep.2001.1543.
Soliman N.F., Nasr S.M., Okbah M.A. 2015. Potential ecological risk of heavy metals in sediments from the Mediterranean coast, Egypt. Journal of Environmental Health Science and Engineering 14 (2): 70. DOI: https://doi.org/10.1186/s40201....
Sreedevi B., Devi M.C., Saigopal D.V.R. 2011. Induction of défense enzymes in Trichoderma harzianum treated groundnut plants against Macrophomina phaseolina. Journal of Biological Control 25 (1): 33–39. DOI: https://doi.org/10.18311/jbc/2....
Treutter D. 2006. Significance of flavonoids in plant resistance: a review. Environmental Chemistry Letters 4: 147–157. DOI: https://doi.org/10.1007/s10311....
Viacava G.E., Roura S.I. 2015. Principal component and hierarchical cluster analysis to select natural elicitors for enhancing phytochemical content and antioxidant activity of lettuce sprouts. Scientia Horticulturae 193: 13–21. DOI: 10.1016/j.scienta.2015.06.041.
Yusnawan E., Inayati A. and Baliadi Y. 2019. Effect of soybean seed treatment with Trichoderma virens on its growth and total phenolic content. In: Proceedings of the International Conference on Biology and Applied Science (ICOBAS) 2120 (1).
Zafra G., Moreno-Montaño A., Absalón Á.E., Cortés-Espinosa D.V. 2015. Degradation of polycyclic aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum. Environmental Science and Pollution Research 22: 1034–1042. DOI: 10.1007/s11356-014-3357-y.
Zahra S., Amin B., Ali M.V.S., Ali Y., Mehdi Y. 2010. The salicylic acid effect on the tomato (Lycopersicum esculentum Mill.) sugar, protein and proline contents under salinity stress (NaCl). Journal of Biophysics and Structural Biology 2 (3): 35–41.
Zeng D., Luo X. 2012. Physiological effects of chitosan coating on wheat growth and activities of protective enzyme with drought tolerance. Open Journal of Soil Science 2 (3): 282–288. DOI: 10.4236/ojss.2012.23034.
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