Management of collar rot of chickpea (Cicer arietinum) by Trichoderma harzianum and plant growth promoting Rhizobacteria
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Department of Botany, Faculty of Science, Banaras Hindu University Varanasi-221005, India
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, India
Corresponding author
Maurya S.
Department of Botany, Faculty of Science, Banaras Hindu University Varanasi-221005, India
Journal of Plant Protection Research 2008;48(3):347-354
Collar rot (Sclerotium rolfsii) of chickpea (Cicer arietinum) is one of the devastating soil-borne diseases of fungal origin, due to which 10–30% yield loss is recorded annually according to severity of the disease. Management of collar rot of chickpea is not feasible in the absence of effective soil fungicides. However, Trichoderma harzianum and plant growth promoting rhizobacteria (PGPR) have shown high efficacy against this disease in vitro as well as in the field. We used T. harzianum (104, 106 and 108 spore/ml) and two PGPRs (Pseudomonas fluorescens strain 4 and P. aeruginosa) as foliar spray with the fresh and heat inactivated microorganisms. Foliar application of T. harzianum (108 spore/ml) and P. fluorescens strain 4 (108 cfu/ml) showed maximum efficacy in reducing plant mortality as compared to the control. Foliar application of fresh-and heat-inactivated (121°C for 10 min) P. fluorescens strain 4, and T. harzianum reduced 15–25% plant mortality but P. aeruginosa showed very little disease control of 10–15%. However, regarding plant growth promotion, it was observed that fresh-and heatinactivated P. fluorescens strain 4 showed maximum efficacy followed by fresh and heat inactivated P. aeruginosa and T. harzianum as compared to the control. The disease-controlling efficacy was also associated with the increase in phenolic acid synthesis in chickpea plants. The control of chickpea collar rot by biocontrol agents is safe and ecologically sound and appears to be a healthy approach to the disease control.
The authors have declared that no conflict of interests exist.
Benhamou N., Chet I. 1996. Parasitism of sclerotia of Sclerotium rolfsii by Trichoderma harzianum: ultrastructural and cytochemical aspects of the interaction. Phytopathology 86: 405–416.
Benhamou N., Rey P., Picard K., Tirilly Y. 1999. Ultrastructural and cytochemical aspects of the interaction between the mycoparasite Pythium oligandrum and soil-borne plant pathogens. Phytopathology 89: 506–517.
Burelle N.K., Kloepper J.W., Reddy M.S. 2006. Plant growth promoting rhizobacterias as transplant amendments and their effects on rhizosphere microorganisms. Appl. Soil Ecol. 31: 91– 100.
Chet I., Harman G.E., Baker R. 1981. Trichoderma hamatum: its hyphal interactions with Rhizoctonia solani and Pythium spp. Microb. Ecol. 7: 29–38.
Chet I. 1987. Trichoderma: Application, mode of action, and potential as a biocontrol agent of soilborne plant pathogenic fungi. p. 137–160. In: “Innovative Approaches to Plant Disease Control (Series in Ecological and Applied Microbiology)” (I. Chet., ed.). J. Wiley & Sons, New York .
Chet I. 1990. Biological control of soil-borne plant pathogens with fungal antagonists in combination with soil treatments. p. 15–25. In: “Biological Control of Soil-borne Plant Pathogens” (D. Hornby, ed.). Wallingford: CAB International.
Davanlou M., Madsen A.M., Madsen C.H., Hockenhull J. 1999. Parasitism of macroconidia, chlamydospores and hyphae of Fusarium culmorum by mycoparasitic Pythium species. Plant Pathol. 48: 352–359.
Elad Y., Chet I., Henis Y. 1983. Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii -scanning electron microscopy and fluorescence microscopy. Phytopathology 73: 85–88.
Elad Y., Chet I., Katan J. 1980. Trichoderma harzianum a biocontrol agent of Sclerotium rolfsii and Rhizoctonia solani. Phytopathology 70: 119–121.
Haas D., Blumer C., Keel C. 2000. Biocontrol ability of fluorescent pseudomonads genetically dissected: importance of positive feedback regulation. Curr. Opin. Biotechnol. 11: 290–297.
Hoitink H.A.J., Boehm M.J. 1999. Biocontrol within the context of soil microbial communities: a substrate- dependent phenomenon. Ann. Rev. Phytopathol. 37: 427–446.
Han D.Y., Coplin D.L., Bauer W.D., Hoitink H.A.J. 2000. A rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance. Phytopathology 90: 327–332.
Harman G.E., Howell C.R., Viterbo A., Chet I., Lorito M. 2004. Trichoderma species-opportunistic, avirulent plant symbionts. Nature Rev. Microbiol. 2: 1–14.
Harman Gary E., Howell Charles R., Ada Viterbo, Chet Ilan, Lorito Matteo. 2004. Trichoderma species – opportunistic, avirulent plant symbionts. Nature Rev. Microbiol. 2: 43–56.
Kerry B.R. 2000. Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Ann. Rev. Phytopathol. 38: 423–441.
Krause M.S., De Ceuster T.J.J., Tiquia S.M., Michel F.C.Jr., Madden L.V., Hoitink H.A.J. 2003. Isolation and characterization of rhizobacteria from composts that suppress the severity of bacterial leaf spot of radish. Phytopathology 93 : 1292–1300.
Krause M.S., Madden L.V., Hoitink H.A.J. 2001. Effect of potting mix microbial carrying capacity on biological control of Rhizoctonia damping-off of radish and Rhizoctonia crown and root rot of poinsettia. Phytopathology 91: 1116–1123.
Maurya S., Rashmi Singh, Singh D.P., Singh H.B., Srivastava J.S., Singh U.P. 2007. Phenolic compounds of Sorghum vulgare in response to Sclerotium rolfsii infection. J. Plant Interact. 2: 25–29.
McBeath J.H., Kirk W.W. 2000. Control of seed-borne late blight on pre-cut potato seed with Trichoderma atroviride. P. 88–97. In “Proceedings of Biocontrol in a New Millennium: Building for the future on past experience”(D. M. Huber, ed.). Purdue University Press, West Lafayette, In.
Sarma B.K., Singh D.P., Mehta S., Singh H.B., Singh U.P. 2002. Plant growth promoting rhizobacteria – elicited alterations in phenolic profile of chickpea (Cicer arietinum) infected by Sclerotium rolfsii. J. Phytopathol. 150: 277–282.
Sivan A., Elhd Y., Chet I. 1984. Biological control effects of a new isolate of Trichoderma harzianum on Pythium aphanidermatum. Phytopathology 74: 498–501.
Sivasithamparam K., Ghisalberti E.L. 1998. Trichoderma and Gliocladium. p. 139–191. (C.P. Kubicek, G. E., eds.). Vol. 1. Taylor and Francis, London.
Yedidia I., Shoresh M., Kerem Z., Benhamou N., Kapulnik Y., Chet I. 2003 Concomitant induction of systemic resistance to Pseudomonas syringae pv. lachrymans in cucumber by Trichoderma asperellum (T-203) and accumulation of phytoalexins. Appl. Environ. Microbiol. 69: 7343–7353.
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