ORIGINAL ARTICLE
Microencapsulation of Eucalyptus globulus essential oil anti-fungal sachet against blue mold on peaches
1
Department of Plant Pathology, University of Poonch, Rawalakot, Pakistan
2
Department of Plant Pathology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
3
Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-05-04
Acceptance date: 2023-07-31
Online publication date: 2023-11-11
Corresponding author
Journal of Plant Protection Research 2023;63(4):428-439
HIGHLIGHTS
- Chitosan Nanoparticles encapsulated with Essential Oil, Biocontrol of Fruit Diseases, Postharvest Pathology
KEYWORDS
TOPICS
ABSTRACT
The present study was specifically designed to develop bio-fungicides that may help mitigate
reliance on hazardous synthetic chemicals which give rise to environmental safety
concerns. A survey (2021–2022) of local fruit markets in Islamabad, Pakistan included
morpho-molecular disease identification. It revealed Penicillium chrysogenum as a major
phytopathogenic fungi causing fruit rot. The fungicidal action of plant essential oils might
be boosted by the technique of application therefore, nanoencapsulation of essential oil
and chitosan was performed. To attain the objective of antimicrobial packaging, essential
oil and chitosan tablets were encapsulated in spun bond sachets against P. chrysogenum
using a sachet volatile phase technique. In vitro screening of nano encapsulated eucalyptus
essential oil showed significant inhibition of radial growth of P. chrysogenum colonies at
1.6 mg · ml–1 followed by 1.4, 1.2 and 1.0 mg · ml–1. Results of Gas Chromatography and
Mass Spectrometry revealed the presence of eucalyptol in eucalyptus EO as a major antifungal
component. An in vivo experiment analyzing the efficacy of essential oil tablets
against pre-inoculated peach fruit with P. chrysogenum at ambient temperatures (7–37°C)
showed significant reduction in lesion diameter, disease severity and prolonged shelf-life
of peaches of more than 2 weeks. The natural ripening process of peach was not affected
by the presence of antifungal sachets as no significant alteration in weight loss of fruit was
recorded. The suppressiveness of fungal mycelial growth of P. chrysogenum was directly
proportional to increases in E. globulus oil concentration. This research may have a significant
impact on prolonging the shelf-life of peach fruit.
RESPONSIBLE EDITOR
Chetan Keswani
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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