ORIGINAL ARTICLE
Molecular Characterization of the Cotton Leaf Curl Multan Betasatellite occurance on Chilli Plants in Multan, Pakistan.
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1
Faculty of Agricultural Sciences & Technology, Department of Plant Pathology, Bahauddin ZakariyaUniversity, Multan, Pakistan
2
Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, Pakistan
3
Faculty of Agricultural Sciences and Technologies, Department of Plant Protection,, Sivas University of Science and Technology, Sivas 58140, Türkiye., Turkey
4
Department of Food Science and Technology, Bahauddin Zakariya University, Multan, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-09-12
Acceptance date: 2024-02-14
Online publication date: 2024-02-22
Corresponding author
Hajra Azeem
Faculty of Agricultural Sciences & Technology, Department of Plant Pathology, Bahauddin ZakariyaUniversity, Multan, Multan, 60800, Multan,, Pakistan
HIGHLIGHTS
- Begomovirus
- Chilli
- Virus on chilli
- Cotton leaf curl virus
- Cotton leaf curl Multan betasatellite
- Geminiviridae
- Phylogenetic analysis
- Leaf curling
- Stunted growth
KEYWORDS
TOPICS
ABSTRACT
The prevalence of Cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan. During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants. Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the region. Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed Begomovirus infection in chili plants. Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively. Sequencing identified Cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili. Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.