Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan
Hajra Azeem 1,2, A-D
Amjad Ali 3, D-F
Rashida Perveen 2, A,E-F
Fatih Ölmez 3, E-F
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Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
Faculty of Agricultural Sciences and Technology, Department of Plant Pathology, Bahauddin Zakariya University, 60800 Multan, Pakistan
Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, Sivas University of Science and Technology, 58140 Sivas, Turkey
Department of Food Science and Technology, Bahauddin Zakariya University, 60000 Multan, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2023-09-12
Acceptance date: 2024-02-14
Online publication date: 2024-05-13
Corresponding author
Hajra Azeem   

Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
  • Begomovirus
  • Chilli
  • Virus on chilli
  • Cotton leaf curl virus
  • Cotton leaf curl Multan betasatellite
  • Geminiviridae
  • Phylogenetic analysis
  • Leaf curling
  • Stunted growth
The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan. During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants. Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the region. Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants. Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively. Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili. Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.
Huan Zhang
The authors have declared that no conflict of interests exist.
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