ORIGINAL ARTICLE
Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan
Hajra Azeem 1,2, A-D
,
 
Amjad Ali 3, D-F
,
 
Rashida Perveen 2, A,E-F
,
 
,
 
,
 
Fatih Ölmez 3, E-F
,
 
 
 
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1
Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
 
2
Faculty of Agricultural Sciences and Technology, Department of Plant Pathology, Bahauddin Zakariya University, 60800 Multan, Pakistan
 
3
Faculty of Agricultural Sciences and Technologies, Department of Plant Protection, Sivas University of Science and Technology, 58140 Sivas, Turkey
 
4
Department of Food Science and Technology, Bahauddin Zakariya University, 60000 Multan, Pakistan
 
 
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
 
 
Submission date: 2023-09-12
 
 
Acceptance date: 2024-02-14
 
 
Online publication date: 2024-05-13
 
 
Corresponding author
Hajra Azeem   

Crop Diseases Research Institute, National Agricultural Research Centre, Islamabad, Pakistan, 45500 Islamabad, Pakistan
 
 
 
HIGHLIGHTS
  • Begomovirus
  • Chilli
  • Virus on chilli
  • Cotton leaf curl virus
  • Cotton leaf curl Multan betasatellite
  • Geminiviridae
  • Phylogenetic analysis
  • Leaf curling
  • Stunted growth
KEYWORDS
TOPICS
ABSTRACT
The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan. During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants. Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the region. Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants. Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively. Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili. Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.
RESPONSIBLE EDITOR
Huan Zhang
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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