Occurrence and distribution of nematodes in rice fields in Guilan province, Iran and the first record of Mylonchulus polonicus (Stefanski, 1915) Cobb, 1917 (Nematoda: Mononchina)
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Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, P.O. Box 4111, Karaj, 31587-11167, Iran
Department of Plant Protection, College of Agriculture, Guilan University, P.O. Box 14115-336, Rasht, Iran
Submission date: 2016-06-27
Acceptance date: 2016-11-21
Corresponding author
Ahmad Kheiri
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, P.O. Box 4111, Karaj, 31587-11167, Iran
Journal of Plant Protection Research 2016;56(4):420-429
The distribution of nematodes was studied in rice fields in Guilan province, Iran, from 2014 to 2016. Nematode biodiversity of 250 soil and root samples was examined. Thirty nematode species were identified morphologically, including plant parasites, microbivores and mycetophagous and predator species. Molecular techniques were also used for further identification of three plant parasitic species. Indicators of population were also estimated. Spiral nematodes (Helicotylenchus crenacauda Sher, 1966 and H. digitiformis Ivanova, 1967) and stunt nematode (Tylenchorhynchus agri) were the predominant parasitic species identified. Among other species, three mononchid species were identified namely Mononchus aquaticus, Mylonchulus sigmaturus and M. polonicus. The species M. polonicus was found and reported in Iran for the first time. The two plant parasitic species T. agri and Xiphinema index were reported in association with rice in Iran for the first time. To evaluate the distribution and incidence of Aphelenchoides besseyi (rice white-tip nematode) in different regions of Guilan province, a total of 255 fresh seed samples were collected/inspected, of which, about 40% of them were infested with A. besseyi. Of the 16 studied counties, the highest percentage of infected seeds came from fields around the city of Astara (69.2%) and the second highest infection was observed near the city of Anzali (60%).
The authors have declared that no conflict of interests exist.
Ahmad W., Jairajpuri M.S. 2010. Mononchida: the Predaceous Nematodes. Series: Nematology monographs and perspectives 7. Brill, Leiden–Boston, Netherlands, 299 pp.
Aliramaji F., Pourjam E., Álvarez-Ortega S., Pedram M., Atighi M.R. 2015. Rotylenchus dalikhaniensis n. sp. (Nematoda: Hoplolaimidae), a monosexual species recovered from the rhizosphere of Ruscus hyrcanus Woronow in Mazandaran province, northern Iran. Nematology 17 (1): 67–77.
Andrássy I. 1992. A taxonomic survey of the family Mylonchulidae (Nematoda). Opuscula Zoologica Budapest 25: 11–35.
Andrássy I. 2009. Free-living nematodes of Hungary (Nematoda errantia), III. Pedozoologica Hungarica 5: 608 pp.
Ashouri M. 2012. The effect of water saving irrigation and nitrogen fertilizer on rice production in paddy fields of Iran production in paddy fields of Iran. International Journal of Bioscience, Biochemistry and Bioinformatics 2 (1): 56–59.
Bridge J., Plowright R.A., Peng D. 2005. Nematode parasites of rice. p. 87–129. In: “Plant Parasitic Nematodes in Subtropical and Tropical Agriculture”. 2nd ed. (M. Luc, R.A. Sikora, J. Bridge, eds.). CAB International, Wallingford, UK, 896 pp.
Coolen W.A., D’Herde C.J. 1972. A method for the Quantitative Extraction of Nematodes from Plant Tissue. State Nematology and Entomology Research Station, Merelbeke, Belgium, 77 pp.
Coomans A., Kheiri A. 1986. Observations on Lindseyus costatus Ferris & Ferris, 1973 with a discussion on its relationships (Nematoda: Belondiridae). Revue De Nematologie 9 (4): 357–367.
De Grisse A.T. 1969. Redescription ou modifications de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen te Gent 34: 351–369.
Elahinia S.A., Mahdavian E. 1998. Geographical distribution of Aphelenchoides besseyi and its infection rate on different cultivars of rice in western parts of Mazandaran and Guilan provinces. p. 86. In: Proceedings of 13th Iranian Plant Protection Congress, 23–27 August, Karaj, Iran, 180 pp. (in Persian, with English summary).
Farahmand S., Eskandari A., Orselli L., Karegar A. 2009. Some known species of the genera Mononchus Bastian, 1865 and Mylonchulus (Cobb, 1916). Nematologia Mediterranea 37 (2): 145–154.
Ferris V.R. 1963. Tylenchorhynchus silvaticus n. sp. and Tylenchorhynchus agri n. sp. (Nematoda: Tylenchida). Proc. Helm. Soc. Wash, 30: 165-168.
Fukano H. 1962. Ecological studies on white tip disease of rice plant caused by Aphelenchoides besseyi, Christie and its control. Bulletin of the Fukuoka Agriculture Experiment Station 18: 1–10.
Ghaderi R., Kashi L., Karegar A. 2012. The Nematodes of Iran (based on the published reports until 2011). Agricultural Education and Extension Publication, Tehran, Iran, 371 pp.
Haidar M.G., Nath R.P., Thakur S.K. 1996. Growth response of rice plant to stunt nematode, Tylenchorhynchus nudus. Journal of Research, Birsa Agricultural University 8 (2): 163–164.
Handoo Z.A. 2000. A key and diagnostic compendium to the species of the genus Tylenchorhynchus Cobb, 1913 (Nematoda: Belonolaimidae). Nematology 32 (1): 20–34.
Handoo Z.A., Palomares-Rius J.E., Cantalapiedra-Navarrete C., Liebanas G., Subbotin S.A., Castillo P. 2014. Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) description of two new species and a molecular phylogeny. Zoological Journal of the Linnean Society 172 (2): 231–264.
Heyns J. 1971. Three Xiphinema species from the south Western Cape Province (Nematoda: Longidoridae). Phytophylactica (plant protection sciences and microbiology) 3 (3): 107–114.
Hopper B.E., Cairns E.J. 1959. Taxonomic Keys to Plant, Soil and Aquatic Nematodes. Alabama Polytechnic Institute, Auburn, AL, USA, 176 pp.
Hunt D.J. 1993. Aphelenchida, Longidoridae and Tricodoridae: Their Systematics and Bionomics. International Wallingford, UK, 352 pp.
Ingham R.E., Trofymow J.A., Ingham E.R., Coleman D.C. 1985. Interactions of bacteria, fungi, and their nematode grazers: effects on nutrient cycling and plant growth. Ecological Monographs 55 (1): 119–140.
Jairajpuri M.S. 1969. Studies on Mononchida of India. I. The genera Hadronchus, Iotonchus and Miconchus and a revised classification of Mononchida, new order. Nematologica 15: 557–581.
Jamali S., Pourjam E., Alizadeh A., Alinia F. 2006. Incidence and distribution of Aphelenchoides besseyi in rice areas in Iran. Journal of Agricultural Science and Technology 2 (2): 337–344.
Jairajpuri M.S., Ahmad W. 1992. Dorylaimida: Free-living, Predaceous and Plant-Parasitic Nematodes. Brill Publishers, Leiden, Netherlands, 458 pp.
Jenkins W.R. 1964. A rapid centrifugal-flotation technique for extracting nematodes from soil. Plant Disease Reporter 48: 692.
Joshi M.M., Hollis J.P. 1976. Pathogenicity of Tylenchorhynchus martini swarmers to rice. Nematologica 22 (1): 123–124.
Khan Z., Araki M. 2002. A new and two first recorded species of mononchids (Nematoda: Mononchida) from Japan. Nematologia Mediterranea 30: 167–173.
Khan A., Jaffry A.H., Khan M.A. 1990. Pathogenicity of Tylenchorhynchus brassicae to three cultivars of rice. International Nematology Network Newsletter 7 (2): 42–43.
Khan A., Shaukat S.S. 2000. Effect of single and simultaneous inoculations of Hirschmanniella oryzae and Tylenchorhynchus annulatus to rice seedlings under laboratory conditions. Sarhad Journal of Agriculture 16 (2): 197–200.
Kheiri A. 1971. Plant-parasitic nematodes (Tylenchida) from Iran. Biologisch Jaarboek Dodonaea 40: 224–239.
Koohkan M., Shokoohi E., Abolafia J. 2014. Study of some mononchids (Mononchida) from Iran with a compendium of the genus Anatonchus. Tropical Zoology 27 (3): 88–127.
Lamberti F., Bleve-Zacheo T., Tuopay D.K., Ciancio A., Boibol J.B. 1987. Relationship between Xiphinema ifacolum and rice in Liberia. Nematologia Mediterranea 15: 303–314.
Loof P.A.A., Luc M. 1990. A revised polytomous key for the identification of species of the genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) with exclusion of the X. americanum group. Systematic Parasitology 16: 35–66.
Minassian V., Barooti S. 1997. First report on occurrence of Hirschmanniella oryzae in Iran. Iranian Journal of Plant Pathology 33: 31–32. (in Persian, with English summary).
Mohammadi A., Alimohammadi A., Habibi R., Shirzadi M.R. 2015. Spatial and statistical analysis of leptospirosis in Guilan province, Iran. The International Archives of the Photogramm. Remote Sensing and Spatial Information Sciences, XL-1/W5:497–502.
Mojtahedi H., Balali G., Akhiani A., Barooti S., Naderi A. 1983. Tylenchorhynchid nematodes of Iran (Tylenchoidea: Nematoda). Iranian Journal of Plant Pathology 19: 11–14. (in Persian, with English summary).
Mousanejad S., Alizadeh A., Safaie N. 2009. Effect of weather factors on spore population dynamics of rice blast fungus in Guilan Province. Journal of Plant Protection Research 49 (3): 319–329.
Mulvey R.H. 1961. The Mononchidae: a family of predaceous nematodes. I. Genus Mylonchulus (Enolpida: Mononchidae). Canadian Journal of Zoology 39: 665–696.
Nickle W.R., Hooper D.J. 1991. Manual of Agricultural Helminthology. Marcel Dekker, New York, USA, 1035 pp.
Norton D.C., Hoffmann J.K. 1974. Distribution of selected plant parasitic nematodes relative to vegetation and edaphic factors. Journal of Nematology 6 (2): 81–86.
Norton D.C. 1978. Ecology of Plant-Parasitic Nematodes. John Wiley and Sons, New York, USA, 268 pp.
Nowruzi R., Barooti S. 2001. Some predatory and plant parasitic nematodes from Bushehr Province, Iran. Applied Entomology and Phytopathology 68 (2): 9–12.
Nunn G.B. 1992. Nematode molecular evolution. Ph.D. thesis, University of Nottingham, Nottingham, UK, 192 pp.
Panahandeh Y., Pourjam E., Aliramaji F., Pedram M. 2015. Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran. Biologia (Section Zoology) 70 (10): 1376–1387.
Pedram M., Pourjam E. 2014. Description of Trichodorus iranicus sp. n. (Diphtherophorina, Trichodoridae) from Iran. Zootaxa 3795 (4): 431–440.
Pedram M., Pourjam E., Panahandeh Y., Soleymanzadeh M., Atighi M.R., Roshan-Bakhsh A., Ehsani A. 2015. Additional data on Iranian trichodorids (Triplonchida: Trichodoridae) and proposal for a new synonymy. Nematology 17 (10): 1185–1191.
Pedramfar H., Pourjam E., Kheiri A. 2001. Plant parasitic nematodes associated with rice in Guilan Province, Iran. Iranian Journal of Plant Pathology 37 (3–4): 285–301. (in Persian, with English summary).
Ravichandra N.G. 2013. Fundamentals of Plant Pathology. Phi Learning, Delhi, India, 652 pp.
Roshan-Bakhsh A., Pourjam E., Pedram M. 2016. Description of Longidorus perangustus sp. n. (Dorylaimida: Longidoridae), an amphimictic species from Iran. European Journal of Plant Pathology 144 (3): 581–594.
Sayari M., Babaeizad V., Ghanbari M.A.T., Rahimian H. 2014. Expression of the pathogenesis related proteins, NH-1, PAL, and lipoxygenase in the Iranian Tarom and Khazar rice cultivars, in reaction to Rhizoctonia solani the causal agent of rice sheath blight. Journal of Plant Protection Research 54 (1): 36–43.
Siddiqi M.R. 2000. Tylenchida Parasites of Plants and Insects. 2nd ed. CAB International, Wallingford, UK, 833 pp.
Soleymanzadeh M., Pedram M., Pourjame E., Álvarez-Ortega S. 2016. Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family. Nematology (in press).
Spedding T.A., Hamel C., Mehuys G.R., Madramootoo C.A. 2004. Soil microbial dynamics in maize-growing soil under different tillage and residue management systems. Soil Biology and Biochemistry 36 (3): 499–512.
Talachian P., Akhiani A. 1976. Occurrence of white tip nematode of rice in Iran. Iranian Journal of Plant Pathology 12: 27. (in Persian, with English summary).
Tanha Maafi Z., Subbotin S.A., Moens M. 2003. Molecular identification of cyst nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology 5 (1): 99–111.
Thorne G., Alen M.W. 1950. Paratylenchus hamatus n. sp. and Xiphinema index n. sp. two new nematodes associated with fig roots, with a note on Paratylenchus anceps Cobb. Journal of the Helminthological Society of Washington 17: 27–35.
Vovlas N., Subbotin S.A., Troccoli A., Liébanas G., Castillo P. 2008. Molecular phylogeny of the genus Rotylenchus (Nematoda, Tylenchida) and description of a new species. Zoologica Scripta 37 (5): 521–537.
Yeates G.W., Coleman D.C. 1982. Role of nematodes in decomposition. p. 55–80. In: “Nematodes in Soil Ecosystems” (D.W. Freckman, ed.). University of Texas Press, Austin, USA, 206 pp.
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