REVIEW
Plant viral disease management-from cross protection to CRISPR
1
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2
Department of Plant Pathology, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-05-09
Acceptance date: 2024-08-28
Online publication date: 2025-06-16
Corresponding author
Nemat Sokhandan-Bashir
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
HIGHLIGHTS
- Biotechnological-based viral plant disease management methods are the most efficient
- Strategies for resistance engineering to plant viruses are constantly evolving
- Syn-tasiRNA and CRISPR are the most efficient methods for creating resistant plants
KEYWORDS
TOPICS
ABSTRACT
In contrast to other plant pathogens, the control of viruses through chemical compounds
is not feasible. Consequently, the management of plant viruses has predominantly relied
on biotechnological approaches rather than those used for other pathogens. This paper
presents a thorough review that takes into account an extensive literature analysis to offer
a comprehensive understanding of biotechnological strategies aimed at developing stable
engineered virus-resistant plants. Examples of these strategies were highlighted in crops,
using as many cases as were available, where these strategies had been used, including cross-
-protection, pathogen-derived resistance (PDR), hairpin RNA, artificial small RNAs, and
genome editing-based CRISPR/Cas systems. In evaluating the trends over time, we have
critically assessed the advantages and disadvantages of each approach, identifying synthetic
trans-acting microRNA (syn-tasiRNA) and CRISPR/Cas as the most effective and precise
methods exhibiting minimal off-target effects on the plant genome. Furthermore, we have
discussed the emerging innovations in non-transgenic resistance strategies such as the application
of double-stranded (ds) RNA which hold promise for overcoming the significant
challenges associated with the commercialization of engineered resistant plants.
ACKNOWLEDGEMENTS
The authors would like to thank Hussein Golafrouz
for his invaluable help in the preparation of Figures 2
and 3.
RESPONSIBLE EDITOR
Julia Minicka
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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