The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying
More details
Hide details
Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection - National Research Institute, ul. Władysława Węgorka 20, 60-318, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-03-27
Acceptance date: 2024-05-17
Online publication date: 2024-05-29
Corresponding author
Joanna Krzymińska   

Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection - National Research Institute, ul. Władysława Węgorka 20, 60-318, Poznań, Poland
  • MG and trehalose preserved yeast's antagonistic abilities during freeze-drying.
  • Treatment efficacy depended on isolate, supplements and timing of the treatment.
  • The interplay between those factors affected the outcome.
  • Isolate 117/10 was the most effective, MG and trehalose preserved its properties.
  • Preventive treatments were generally more effective than intervention.
In the present eco-conscious era, consumers opt for food choices reflecting ethical and environmental concerns, which increases the demand for organic products. Biocontrol is a viable plant protection method in organic farming. Freeze-drying is a long-term preservation technique for microbial agents, ensuring their genetic stability and viability. To reduce freeze-drying-induced damage to their cells, cryoprotective agents like trehalose and monosodium glutamate are used. This study evaluated the impact of the addition of these substances during the freeze-drying process on chosen yeast isolates' viability, their ability to survive on tomato leaves and maintain antagonistic properties against Botrytis cinerea Pers. Yeast isolates 114/73 (Wickerhamomyces anomalus E.C. Hansen) and 117/10 (Naganishia albidosimilis Vishniac & Kurtzman) were tested on tomato plants under greenhouse conditions before and after the freeze-drying process for both the ability to colonize leaves and as a preventive and interventional treatment against B. cinerea. Yeast viability post freeze-drying was evaluated in vitro. Both trehalose and monosodium glutamate increased yeast viability during the freeze-drying process. Viability was not very high (from 30.33 to 36.17% for 117/10 and from 10.67 to 16.5% for 114/73). Yeast dehydrated after freeze-drying, protected with trehalose and monosodium glutamate, displayed the same colony count on tomato leaves as before freeze-drying. The efficacy of protective treatments depended on the yeast isolate, the protective substance used during freeze-drying, treatment timing (prevention vs. intervention), and interactions of those factors. Cryopreserved isolate 117/10 performed better than 114/73 with the addition of either trehalose or monosodium glutamate, reducing the disease severity index from 88.3% (control) to 18.75-55.33%. Preventive treatments were more efficient than intervention. The leaf colonization ability and biocontrol efficacy of yeast isolates against B. cinerea post-freeze-drying offer promising solutions in sustainable agriculture. However, further research, to analyze the interactions between various factors and to optimize strategies may be needed.
The authors have declared that no conflict of interests exist.
Journals System - logo
Scroll to top