• This paper talks about friendly environmental fight against Phytophthora colocasiae, using fluorescent Pseudomonas rhizobacteria. It is research article providing new outcomes about immunization and growth stimulation of taro by Pseudomonas rhizobacteria in Cameroon. This is the first manuscript about the potential use of fluorescent Pseudomonas, in the fight against P. colocasiae.
Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future.
The authors are grateful to the University of Dschang and the research unit of Natural Substances, (University of Douala) for facilities and some consumables used in this work.
Krzysztof Krawczyk
The authors have declared that no conflict of interests exist.
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