ORIGINAL ARTICLE
Antifungal activity of Bacillus spp. against Fusarium oxysporum f. sp. lycopersici and Ascochyta sp.
1
Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria
2
Department of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, Oran, Algeria
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-02-04
Acceptance date: 2022-04-19
Online publication date: 2022-08-11
Corresponding author
Journal of Plant Protection Research 2022;62(3):247–257
HIGHLIGHTS
- Five strains of Bacillus spp. isolated from soil have the ability to inhibit two different phytopathogens Fusarium oxysporum f. sp. lycopersici and Ascochyta sp.
- Four of the five strains belong to the species B. halotolerans and one to the species B. cereus
- The cell-free filtrates and the volatiles compound produced by these strains slowed down the growth of the phytopathogens
KEYWORDS
TOPICS
ABSTRACT
The aim of this study is to find bacterial strains with antagonistic effects against Fusarium
oxysporum f. sp. lycopersici (Fol) and Ascochyta sp, which are phytopathogens responsible
for fusarium wilt of tomato and ascochyta blight of peas, respectively. One hundred thirty-
six bacteria isolated from the rhizosphere of tomatoes were screened. Five strains with
the largest inhibition zones were selected. These strains were identified by the phenotypic
method, later confirmed by sequencing of 16S rDNA. All strains belonged to the genus
Bacillus spp. Their inhibition capacity was evaluated by the direct method by doing a dual
culture, the inhibition rates ranged from 44.32 ± 0.8 to 61.36 ± 0.2 against Fol and 62.04 ± 0.8
to 74.1 ± 0.2% against Ascochyta sp. They were then evaluated by the indirect method by
evaluating, on one hand, the antifungal effect of the volatile compounds produced by the
strains and on the other hand, the antifungal effect of the filtrates. The results showed that
volatile compounds inhibited plant pathogens’ growth with average inhibition rates of
55% against Fol and 17% against Ascochyta. For filtrates, the average inhibition rates were
33.01% against Fol and 33.74% against Ascochyta sp. Finally, the plant growth promoting
rhizobacteria (PGPR) effect of B. halotolerans RFP57 was evaluated. This involved assessing
their ability to stimulate the germination of tomato seeds and the growth of their vegetative
organs. The results showed significant improvement in treated seedlings compared to
controls. All these results show that the strains selected for this study have the potential for
use as a biocontrol agent. However, it is clear that further in-depth studies are needed to
confirm their true potentiality.
RESPONSIBLE EDITOR
Sebastian Stenglein
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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