ORIGINAL ARTICLE
Comparison of infection caused by Pythium debaryanum, Phytophthora palmivora and Erwinia caricae on transgenic and non-transgenic papaya ringspot virus resistant plants
 
More details
Hide details
1
Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños, Laguna, 4031 Philippines
2
Crop Science Cluster, College of Agriculture, University of the Philippines Los Baños, College, Laguna, 4031 Philippines
CORRESPONDING AUTHOR
Cumagun Christian Joseph Rili
Crop Protection Cluster, College of Agriculture, University of the Philippines Los Baños, Laguna, 4031 Philippines
 
Journal of Plant Protection Research 2008;48(2):137–145
KEYWORDS
TOPICS
ABSTRACT
The study was conducted to determine the reaction of transgenic papaya ringspot virus (PRSV) – resistant papaya to some major fungal and bacterial diseases of papaya. Four lines of transgenic papaya developed by the Institute of Plant Breeding (IPB) and non-transgenic ‘Davao solo’ were used in the screening. Plants were artificially inoculated with the most aggressive isolates in a contained greenhouse. Inoculation was done by incorporating Pythium debaryanum and Phytophthora palmivora – colonized wheat seeds into the base of the plant and by pricking the base of leaf petiole with a needle dipped in suspension of Erwinia caricae. Four transgenic papaya lines and ‘Davao Solo’ were susceptible to P. debaryanum and P. palmivora under greenhouse conditions. No significant differences in per cent wilted seedlings caused by P. palmivora among transgenic four lines and ‘Davao Solo’ were observed (p = 0.05). Similarly, no differences in root rot severity (%) among the same test plants due to P. palmivora were noted. The three transgenic papaya lines were initially more susceptible than ‘Davao Solo’ to E. caricae seven days after inoculation but the same degree of infection was attained 14 days after inoculation. The transgenic and the non-transgenic papaya exhibited susceptible reactions to P. debaryanum in the greenhouse. No significant difference was observed in one transgenic line and ‘Davao Solo’ in terms of pre and post germination damping-off incidence in inoculated soil (p = 0.05).
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (12)
1.
Colyer P.D., Kirkpatrick T.L., Caldwell W.D., Vernon P.R. 1999. Expression of root-knot nematode/Fusarium wilt resistance in transgenic cotton cultivars. Phytopathology 89, p. 17.
 
2.
Drenth A., Sendall B. 2001. Practical guide to detection and identification of Phytophthora, Version 1.0. CRC for Tropical Plant Protection. Brisbane, Australia.
 
3.
Galinsky R. 1996. World Market for Papaya. Regional Agribusiness Project Market Information Bulletin. USAID-Asia RAP. No. 12, 5 pp.
 
4.
Gardan L., Christen R., Achouak W., Prior P. 2004. Erwinia papayae sp. nov., a pathogen of papaya (Carica papaya). In. J. Syst. Evol. Microbiology 54: 107–113.
 
5.
Lee B.S., Varghese G. 1974. Studies on the genus Phytophthora in Malaysia. I. Isolation techniques, comparative morphology and physiology and reaction to antibiotic. Malaysian Agricultural Research 3: 13–21.
 
6.
Manshardt R.M. 1999. Papaya. p 489–511. In: “Biotechnology of Perennial Fruit Crops” (F.A. Hammerschlag and R.E. Lits. eds.). Vol. 21. CAB International, Wallingford, U.K.
 
7.
Mclean K.S., Lawrence G.W., Gazaway W.S., Palmateer A.J., Akridge J.R. 2001. Field response of transgenic and non-transgenic cotton varieties to the reniform nematode. Phytopathology 9, p. 138.
 
8.
Obrero F.P. 1991. Bacterial crown rot of papaya in the Philippines. p. 20–23. In: Proceedings First National Symposium/Workshop “Ringspot and Other Diseases of Papaya in the Philippines”. 7–8 February 1991, The Philippine Phyt. Soc. Bureau of Plant Industry, San Andres, Malate, Manila.
 
9.
Ploetz R.C., Zentmyer G.A., Nishijima W.T., Rohrbach K.G., Ohr H.D. 1994. Compendium of Tropical Fruit Diseases. Am. Phytopathology Soc. St. Paul, MN. 111 pp.
 
10.
Tangonan N.C., Quebral F.C. 1992. Host Index of Plant Diseases in the Philippines. 2nd ed. Department of Science and Technology Bicutan, Taguig, Manila, 273 pp.
 
11.
Utz H.F. 2000. PLABSTAT. A computer program for statistical analysis of plant breeding experiments. Version 2N. Institute of Plant Breeding, Seed Science and Population Genetics. University of Hohenheim, Stuttgart, Germany.
 
12.
Wei X., Zou H., Chu L., Liao B., Ye C., Lan C. 2006. Field released transgenic papaya affects microbial communities and enzyme activities in soil. Plant and Soil 285 (1/2): 347–358.
 
eISSN:1899-007X
ISSN:1427-4345