ORIGINAL ARTICLE
 
HIGHLIGHTS
  • This is the first report of DsMV in Poland.
  • DsMV population is highly diverse.
  • Variability of DsMV was confirmed by low sequence identity and recombination events.
  • On phylogenetic tree the Polish isolates grouped separately from others DsMV isolates.
KEYWORDS
TOPICS
ABSTRACT
Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterisation of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene (CP) of the Polish and other DsMV isolates described to date. The pre- sence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of pri- mers were designed and used in the RT-PCR. The specificity of obtained products was con- firmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. The phylogenetic analysis was performed based on 37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease management relies strongly on a fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland.
FUNDING
This study was supported by the Polish Ministry of Education and Science, by Statutory activity project: WIB03.
RESPONSIBLE EDITOR
Yongzhi Wang
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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