ORIGINAL ARTICLE
Detection and genetic variability of newly identified dasheen mosaic virus in Poland
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Julia Minicka 1, B,E-F
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Daria Budzyńska 1, B,E-F
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1
Department of Virology and Bacteriology, Institute of Plant Protection–National Research Institute, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Beata Hasiów-Jaroszewska   

Department of Virology and Bacteriology, Institute of Plant Protection–National Research Institute, Węgorka 20, 60-318, Poznan, Poland
Submission date: 2022-04-26
Acceptance date: 2022-08-08
Online publication date: 2022-09-02
 
 
HIGHLIGHTS
  • This is the first report of DsMV in Poland.
  • DsMV population is highly diverse.
  • Variability of DsMV was confirmed by low sequence identity and recombination events.
  • On phylogenetic tree the Polish isolates grouped separately from others DsMV isolates.
KEYWORDS
TOPICS
ABSTRACT
Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and can cause major economic losses for ornamental crops. Here, we present the detection and molecular characterization of DsMV isolates originating from Monstera adansonii plants in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the coat protein gene (CP) of the Polish and other DsMV isolates described to date. The presence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse transcription- polymerase chain reaction (RT-PCR) with specific, diagnostic primers in three out of ten examined plants. To obtain full-length sequences of CP, two pairs of primers were designed and used in the RT-PCR. The specificity of the obtained products was confirmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other DsMV sequences retrieved from the GenBank. Analyses revealed that the DsMV population is very diverse. The variability of DsMV isolates was confirmed by low sequence identity and pervasive recombination events. Phylogenetic analysis was performed based on 37 non-recombinant CP sequences. Maximum-likelihood reconstruction revealed that the Polish isolates were distinct and grouped separately from other DsMV isolates. Due to high genetic diversity, detecting the virus could be difficult. Nevertheless disease management relies strongly on fast and accurate identification of the causal agent. To our knowledge this is the first report of DsMV in Poland.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
eISSN:1899-007X
ISSN:1427-4345