Effect of Glomus mosseae (arbuscular mycorrhizal fungus) on host - parasite relationship of Meloidogyne incognita (southern root-knot nematode) on four improved cowpea varieties
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Department of Crop Protection, University of Agriculture P.M.B. 2240, Abeokuta, Nigeria
College of Agricultural Sciences, Yewa Campus, Olabisi Onabanjo University, P.M.B. 0012, Ago-Iwoye, Nigeria
Odeyemi Ishola Segun
Department of Crop Protection, University of Agriculture P.M.B. 2240, Abeokuta, Nigeria
Journal of Plant Protection Research 2010;50(3):320–325
Two pot experiments and a field study were conducted in a Randomized Complete Block Design (RCBD). The experiments were conducted to determine the effect of Glomus mosseae, a mycorrhiza fungus, on the reaction of four improved cowpea varieties to Meloidogyne incognita. Cowpea plants were inoculated with a single or a combination of 5 000 eggs of M. incognita and 50 g of G. mosseae inoculum containing 5 spores/g of soil. The standardized method of screening and reporting resistance of crop germplasm to root-knot nematodes at 60 days after planting, and the modified version of including yield for resistance rating at harvest were used for this study. Root galling due to M. incognita infection was significantly lower on all the cowpea varieties treated with G. mosseae and more significantly on IT90K-277-2 and IT89KD-288 in the screenhouse. G. mosseae, suppressed root-knot nematode reproduction on all the varieties compared to cowpea plants infected only by M. incognita both in the screenhouse and field experiments. Also, G. mosseae mitigated the damage attributable to the root-knot nematode on all these varieties. Using Gall Index (GI), reproduction factor and yield, G. mosseae was effective in improving the resistance of the cowpea varieties to M. incognita. IT90K-76 cowpea variety was consistently resistant to the root-knot nematode, while IT90K-277-2 was tolerant with M. incognita infection but resistant with G. mosseae treatment. IT90K-941-1 variety was resistant in the screenhouse. The results of this study also confirmed G. mosseae as a potential bio-control agent for M. incognita on these cowpea varieties.
The authors have declared that no conflict of interests exist.
Adegbite. A.A., Agbaje, G.O., Abidoye J. 2008. Assessment of yield loss of roselle (hibiscus sabdariffa l.) due to root-knot nematode, meloidogyne incognita under field conditions. J. Plant Protection Res. 48 (3): 267–273.
Adesiyan S.O, Caveness F.E., Adeniyi M.O., Fawole B. 1990. Nematode Pests of Tropical Crops. Heinemann Educational Books, Nigeria, 114 pp.
Afolami S.O. 2000. Sugesstions for improvement of current methods of studying and reporting resistance to root-knot nematodes. Int. J. Nematol. 10: 94–100.
Afolami C.A. 2002. An imperical analysis of variety price premium attributes, spatial and temporal pricing patterns for cowpeas in Ogun State, Nigeria. Afr. Crop Sci. J. 10 (3): 263–270.
Barea J.M., Jeffries P. 1995. Arbuscular mycorrhiza in sustainable soil plant systems. p. 521–559. In: “Mycorrhiza Structure, Function, Molecular Biological Biotechnology” (B. Hock, A. Varma, eds.). Springer, 704 pp.
Bethlenfalvay G.I., Linderman R.G. 1992. Mycorrhizae in Sustainable Agriculture. ASA, Madison, Wis., 124 pp.
Boultler D., Evans I.M., Thompson A., Yardwood A. 1975. The amino acid composition of Vigna unguiculata meal in relation to nutrition. In: “Proceedings of Symposium on Nutritional Improvement of Food Legumes by Breeding” (M. Miller, ed.). 3–5 July 1975, United Nations Protein Advisory Group, New York.
Cabamillas E., Baker K. 1989. Impact of Paccilomyces lilacinon level and application time on control of Meloidogyne level and application time on control of Meloidogyne incognita on tomato. J. Nematol 21 (1): 115–120.
Carling D.E., Roncadori R.W., Hussey R.S. 1995. Interactions of arbuscular mycorrhizae, Meloidogyne arenaria and phosphorus fertilization on peanut. Mycorrhiza 6 (1): 9–13.
Caveness F.E. 1992. Nematological research at IITA. A summary of investigation conducted between 1969 and 1988. p. 17–19. In: “Plant Health Management Research Monograph” (J. Lowe, ed.).
Doncaster C.C. 1962. A counting dish for nematodes. Nematologica 7: 334–336.
Faye M., DeBoer J.L., Sene A., Ndiaye M. 2002. Identifying cowpea characteristics which command price premium in Senegalese markets. p. 424–435. In: “Challenges and Oppurtunities for Enhancing Sustainable Cowpea Production” (C.A. Fatokun, S.A. Tarawali, B.B. Singh, P.M. Kormawa, M. Tamo, eds.). IITA, 433.
Florini D.A. 1997. Nematodes and other pathogens of cowpea. p. 193–206. In: “Advances in Cowpea Research” ( B.B. Singh, D.R Mohan Raj, K.E. Dashiell, L.E.N Jakai, eds.). Proceedings of the 2nd World Cowpea Research Conference. Co-publication of IITA and JIRCAS. 3–7 September 1995, Accra, Ghana.
Hussey R.S., Roncadori R.W. 1982. Vesicular – arbuscular mycorrhizae may limit nematode activity and improve plant growth. Plant Dis. 66 (1): 9–14.
Hussey R.S., Barker K.R. 1973. A comparison of methods of collecting inocula of Meloidogyne spp, including a new technique. Plant Dis. 57: 1025–1028.
Karajeh M.R. 2008. Interaction of root-knot nematode (meloidogyne javanica) and tomato as affected by hydrogen peroxide. 2008. J. Plant Protection Res. 48. (2): 182–187.
Koch K.A., Hussey R.S., Roncandori R.W. 1997. Interacions between vesicular – arbuscular mycorrhizal fungi and plant pathogens. Phytopathology 77: 115–119.
Korayem A.M., Youssef M.M.A., Mohamed M.M.M. 2008. Effect of chitin and abamectin on meloidogyne incognita infesting rapeseed. J. Plant Protection Res. 43 (3): 365–370.
Linderman R.G. 1994. Role of VAM fungi in biocontrol. p. 1–26. In: “Mycorrhizae and Plant Health” (F.L. Pfeger, R.G. Linderman, eds.). APS Press, St Paul, MN, USA, 360 pp.
Maareg M.F., Badr S.T.A. 2000. The effect of certain biocontrol organisms, oxamyl and their combination on Meloidogyne javanica infecting sugar beet. Egyptian J. Agronematology 4 (1–2): 95–104.
Oclarit E.L., Cumagun C.J.R. 2009. Evaluation of efficacy of Paecilomyces lilacinus as biological control agent of Meloidogyne incognita attacking tomato. J. Plant Protection Res. 49 (4): 337–340.
Oostenbrink M. 1966. Major characteristics of the relation between nematodes and plants. Meded. Landbouwhogesch. Wageningen 66: 1–46.
Philips J.M., Hayman D.S. 1970. Improved procedure for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 54: 53–63.
Quin F.M. 1997. Introduction in Advances in Cowpea Research. edited by Singh B.B., Mohan Raj D.R., Dashell K.E., Jackaiu L.E.N., Sayce Publishing Devenon, UK.
Rachie K.O. 1985. Introduction. p. 21–28. In: “Cowpea Research, Production and Utilizations” (S.R Singh, K.O. Rachie, eds.). World Cowpea Research Conference, International Institute of Tropical Agriculture.
Sasser J.N., Carter C.C., Hartman K.M. 1984. Standardization of Host Suitability Studies and Reporting of Resistance to Root-Knot Nematodes. A cooperative publication of the Dept. of Plant Pathology, North Carolina, State University and USAID, 7 pp.
Sivaprasad P., Jacob A., George B. 1990. Root-knot nematode infestation and nodulation as influenced by VA mycorrhiza association in cowpea. Indian J. Nematol. 20 (1): 49–52.
Smith G.S. 1987. Interactions of nematodes with mycorrhizal fungi. p. 292–300. In: “Vistas on Nematology – A Commemoration of the Twenty-fifth” (J.A Veech, D.W. Dickson, eds.). Society of Nematolosis, Inc. Hyattsville, MD, USA.
Thompson R. 1998. Public policy for suitable agriculture and rural equity. Foods Policy 23 (1): 1–7.
Whitehead A.G., Hemming J.R. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Ann. Appl. Biol. 55: 25–38.
Youssef M.M.A., Wafaa M.A., El-Nagdi W.M.A., Abi El-Fattah A.I. 2008. Efficacy of chicken compost, Bacillus thuringiensis and Pseudomonas fluorescens for biocontrol of Meloidogyne incognita infecting sugar beet in Egypt. Int. J. Nematol. 18 (1): 35–40.