Effect of some fungicides against the growth inhibition of Sclerotinia sclerotiorum mycelial compatibility groups
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Department of Plant Protection Research, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, P.O. Box 48175-556, Sari, Iran
Department of Plant Protection Research, Damghan Azad University, P.O. Box 36716-39998, Damghan, Iran
Alireza Dalili
Department of Plant Protection Research, Mazandaran Agricultural and Natural Resources Research and Education Center, AREEO, P.O. Box 48175-556, Sari, Iran
Submission date: 2015-05-27
Acceptance date: 2015-10-13
Journal of Plant Protection Research 2015;55(4):354–361
Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of Sclerotinia stem rot, is one of the most important pathogens of Brassica napus L. in northern Iran. In this study, 13 mycelial compatibility groups (MCGs) of the fungus were identified among 31 isolates sampled from four regions of Mazandaran province, Iran. Effective fungicides are useful in the integrated management of the disease. So, the effect of tebuconazole, propiconazole, cyproconazole, and Rovral-TS at five doses (0.0001, 0.001, 0.01, 0.1, and 1 ppm) was studied on the growth inhibition of S. sclerotiorum as in vitro tests. Maximum inhibition (100%) of S. sclerotiorum mycelial growth was obtained by the highest dose (1 ppm) of all tested fungicides, as well as by the doses of 0.1 and 0.01 ppm of propiconazole, cyproconazole, and tebuconazole. In this investigation, the reaction of S. sclerotiorum isolates belonging to different MCGs was evaluated against tebuconazole, propiconazole, cyproconazole, and Rovral-TS at their EC 50 ranges. The results revealed that there was high variation of S. sclerotiorum MCGs against different fungicides. The inhibition percentage varied between 4.29% and 71.72%
The authors have declared that no conflict of interests exist.
Attanayake R.N., Carter P.A., Jiang D., del Río-Mendoza L., Chen W. 2013. Sclerotinia sclerotiorum populations infecting canola from China and the United States are genetically and phenotypically distinct. Phytopathology 103 (7): 750–761.
Boland G.J., Hall R. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology 16 (2): 93–108.Bradley C.A., Lamey H.A., Endres G.J., Henson R.A., Hanson B.K., McKay K.R., Halvorson M., LeGare D.G., Porter P.M. 2006. Efficacy of fungicides for control of Sclerotinia stem rot of Canola. Plant Disease 90 (9): 1129–1134.
Brent K.J., Hollomon D.W. 2007. Fungicide Resistance in Crop Pathogens: How Can it be Managed? Fungicide Resistance Action Committee, Brussels, Belgium, 56 pp.
Carbone I., Anderson J.B., Kohn L.M. 1999. Patterns of descent in clonal lineages and their multilocus fingerprints are resolved with combined gene genealogies. Evolution 53 (1): 11–21.
Duan Y., Ge C., Liu S., Chen C., Zhou M. 2013. Effect of phenylpyrrole fungicide fludioxonil on morphological and physiological characteristics of Sclerotinia sclerotiorum. Pesticide Biochemistry and Physiology 106 (1–2): 61–67.
Garg H., Sivasithamparam K., Banga S.S., Barbetti M.J. 2008. Cotyledon assay as a rapid and reliable method of screening for resistance against Sclerotinia sclerotiorum in Brassica napus genotypes. Australasian Plant Pathology 37 (2): 106–111.
Glass N.L., Kuldau G.A. 1992. Mating type and vegetative incompatibility in filamentous ascomycetes. Annual Review of Phytopathology 30: 201–224.
Glass N.L., Kaneko I. 2003. Fatal attraction: nonself recognition and heterokaryon incompatibility in filamentous fungi. Eukaryotic Cell 2 (1): 1–8.
Gossen B.D., Rimmer S.R., Holley J.D. 2001. First report of resistance to benomyl fungicide in Sclerotinia sclerotiorum. Plant Disease 85 (11): 1206.
Irani H., Heydari A., Javan-Nikkhah M., İbrahimov A.Ş. 2011. Pathogenicity variation and mycelial compatibility groups in Sclerotinia sclerotiorum. Journal of Plant Protection Research 51 (4): 329–336.
Kohli Y., Morrall R.A.A., Anderson J.B., Kohn L.M. 1992. Local and trans-Canadian clonal distribution of Sclerotinia sclerotiorum on canola. Phytopathology 82 (8): 875–880.
Kohn L.M., Carbone I., Anderson J.B. 1990. Mycelial interactions in Sclerotinia sclerotiorum. Experimental Mycology 14 (3): 255–267.
Kuang J., Hou Y.P., Wang J.X., Zhou M.G. 2011. Sensitivity of Sclerotinia sclerotiorum to fludioxonil: In vitro determination of baseline sensitivity and resistance risk. Crop Protection 30 (7): 876–882.
Kull L.S., Pedersen W.L., Palmquist D., Hartman G.L. 2004. Mycelial compatibility grouping and aggressiveness of Sclerotinia sclerotiorum. Plant Disease 88 (4): 325–332.
Liu X., Yin Y., Yan L., Michailides T.J., Ma Z. 2009. Sensitivity to iprodione and boscalid of Sclerotinia sclerotiorum isolates collected from rapeseed in China. Pesticide Biochemistry and Physiology 95 (2): 106–112.
Lobato K.R., Cardoso C.C., Binfaré R.W., Budni J., Wagner C.L.R., Brocardo P.S., de Souza L.F., Brocardo C., Flesch S., Freitas A.E., Dafré A.L., Rodrigues A.L.S. 2010. α-Tocopherol administration produces an antidepressant-like effect in predictive animal models of depression. Behavioural Brain Research 209 (2): 249–259.
Lu G. 2003. Engineering Sclerotinia sclerotiorum resistance in oil seed crops. African Journal of Biotechnology 2 (12): 509–516.
Ma H.X., Chen Y., Wang J.X., Yu W.Y., Tang Z.H., Chen C.J., Zhou M.G. 2009. Activity of carbendazim, dimethachlon, iprodione, procymidone and boscalid against Sclerotinia stem rot in Jiangsu province of China. Phytoparasitica 37 (5): 421–429.
Mueller D.S., Dorrance A.E., Derksen R.C., Ozkan E., Kurle J.E., Grau C.R., Gaska J.M., Hartman G.L., Bradley C.A., Pedersen W.L. 2002. Efficacy of fungicides on Sclerotinia sclerotiorum and their potential for control of Sclerotinia stem rot on soybean. Plant Disease 86 (1): 26–31.
Pandey D.K., Tripathi N.N., Tripathi R.D., Dixit S.N. 1982. Fungitoxic and phytotoxic properties of the essential oil of Hyptis suaveolens. Zeitschrift für Pflanzenkrankheit und Pflanzenschutz 89 (6): 344–349.
Purdy L.H. 1979.Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology 69 (8): 875–880.
Qin H., Chen F., Fu D., Gao X., Huang L., Han Q., Kang Z., Xiong X., Zhang F. 2011. [Sensitivity of Sclerotinia sclerotiorium to 10 fungicides and controlling effect of different medicaments to the rape Sclerotinia stem rot in field]. Journal of Northwest A & F University (Natural Science Edition) 39 (7): 117–122. (in Chinese, with English summary).
Schafer M.R., Kohn L.M. 2006. An optimized method for mycelial compatibility testing in Sclerotinia sclerotiorum. Mycologia 98 (4): 593–597.
Shi Z., Zhou M., Ye Z., Shi J., Chen H., Wang Y. 2000. [Resistance monitoring of Sclerotinia sclerotiorum to carbendazim]. Jiangsu Journal of Agricultural Sciences 16: 226–229. (in Chinese, with English summary).
Steel C.C., Nair N.G. 1993. The physiological basis of resistance to the dicarboximide fungicide iprodione in Botrytis cinerea. Pesticide Biochemistry and Physiology 47 (1): 60–68.
Wang C., Liu W., Ding S., Wang Z., Zhang Y. 2010. [Study on function of tebuconazole on Sclerotinia sclerotiorum]. Chinese Journal of Oil Crop Sciences 32 (3): 436–440. (in Chinese, with English summary).
Zhao J., Peltier A.J., Meng J., Osborn T.C., Grau C.R. 2004. Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napususing petiole inoculation technique under green-house conditions. Plant Disease 88 (9): 1033–1039.