Influence of Fusarium oxysporum f. sp. Cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol production by Pseudomonas fluorescens Migula in banana rhizosphere
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Tamil Nadu Agricultural University, Agricultural Research Station Kovilpatti – 628 501, Tamil Nadu, India
Agricultural College and Research Institute, Killikulam – 628 252, India
Thangavelu Saravanan
Tamil Nadu Agricultural University, Agricultural Research Station Kovilpatti – 628 501, Tamil Nadu, India
Journal of Plant Protection Research 2006;46(3):241–253
Influence of Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol (DAPG) production in the rhizosphere of banana cultivar Rasthali by Pseudomonas fluorescens was investigated. The purified extracts of Pfm strain of P. fluorescens isolated from banana rhizosphere inhibited the growth and spore germination of F. oxysporum f. sp. cubense under laboratory conditions. DAPG extracted from the cultures of the strain was observed as distinct spots in thin layer chromatographic plates at Rf value of 0.88. The extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense eluted at retention time ranges from 20.00 min to 21.30 min. The quantity of DPAG production was less in the extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense as compared to P. fluorescens alone inoculated soil. The talc formulation of Pfm strain also reduced vascular discolouration due to the pathogen in banana plants when inoculated at 15 g/plant.
The authors have declared that no conflict of interests exist.
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