ORIGINAL ARTICLE
Molecular detection of Cucumber mosaic virus from Basella alba, Telfairia occidentalis and Talinum fruticosum in Nigeria
 
 
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Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Adedapo Olutola Adediji   

Department of Crop Protection and Environmental Biology, University of Ibadan, Agbowo, 200222, Ibadan, Nigeria
Online publish date: 2019-06-27
Submission date: 2018-09-14
Acceptance date: 2019-05-29
 
Journal of Plant Protection Research 2019;59(2):177–184
KEYWORDS
TOPICS
ABSTRACT
Cucumber mosaic virus (CMV; family Bromoviridae, genus Cucumovirus) is the most cosmopolitan plant virus occurring worldwide. In the present study, leaf samples showing deformations, mosaics, and chlorotic spots symptoms were collected from naturally infected Basella alba, Telfairia occidentalis and Talinum fruticosum in a home yard garden in Ibadan, Nigeria. Total nucleic acid was extracted from leaves and used as template for cDNA synthesis. RT-PCR was carried out using CMV-specific primers targeting RNA-1 segment. Samples were also tested by RT-PCR using Potyvirus and Begomovirus genusspecific primers. DNA fragments with the expected sizes of ~500 bp were amplified by using CMV-specific primers; however, the expected amplicons were not produced using specific primers used for the detection of potyviruses and begomoviruses. The nucleotide and deduced amino acid sequences obtained for the isolates studied contained 503–511 nt and 144 aa, respectively. The isolates shared 81.9–85.3% nucleotide and 74.3–77.8% amino acid sequence identities with each other. The results of BLASTN analyses showed the highest identities of the isolates (80–93%) with CMV strains from Japan, USA and South Korea. Alignment of deduced partial protein revealed multiple amino acid substitutions within the three isolates and high identities with CMV subgroup I. Phylogenetic analyses putatively categorized the isolates in close association with subgroup IB isolates. The three isolates clustered together into a separate subclade, indicating possible new CMV strains. The results provide the first molecular evidence for CMV infections of T. fruticosum and B. alba in Nigeria and seem to show the possible presence of new strain(s). These findings also add three new hosts to the list of natural host range of the virus in Nigeria.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (48)
1.
Abarshi M.M., Mohammed I.U., Wasswa P., Hillocks R.J., Holt J., Legg J.P., Seal S.E., Maruthi M.N. 2010. Optimization of diagnostic RT-PCR protocols and sampling procedures for the reliable and cost-effective detection of Cassava brown streak virus. Journal of Virological Methods 163: 353–359. DOI: https://doi.org/10.1016/j.jvir....
 
2.
Aliyu T.H., Balogun O.S., Osumah R., Adeoti O.M. 2014. Pathogenicity of Cucumber mosaic virus (CMV) and putative Bitterleaf mosaic virus (BLMV) on Celosia argentea. Taraba Journal of Agricultural Research 2 (1): 49−53.
 
3.
Arogundade O., Balogun O.S., Sholaku O., Aliyu T.H. 2010. Influence of Cowpea mottle virus and Cucumber mosaic virus on the growth and yield of six lines of soybean (Glycine max L.). Journal of Agricultural Science 2 (1): 72–78. DOI: https://doi.org/10.5539/jas.v2....
 
4.
Atiri G.I. 1985. An isolate of Cucumber mosaic virus from fluted pumpkin in Nigeria. Phytopathologische Zeitschrift 114 (3): 268−273. DOI: https://doi.org/10.1111/j.1439....
 
5.
Atiri G.I. 1990. Relationships between growth stages, leaf curl symptom development and fruit yield in okra. Scientia Horticulturae 54 (2): 49–53. DOI: https://doi.org/10.1016/0304-4....
 
6.
Ayo-John E.I., Hughes J.d’A. 2014. Identification of Cucumber mosaic virus (CMV) isolates infecting Musa spp. and vegetable crops in southern Nigeria. International Journal of Virology 10 (3): 204−210. DOI: https://doi.org/10.3923/ijv.20....
 
7.
Bujarski J., Figlerowicz M., Gallitelli D., Roossinck M.J., Scott S.W. 2012. Family Bromoviridae. p. 965−976. In: “Virus Taxonomy Classification and Nomenclature of Viruses. Ninth Report of the International Committee on Taxonomy of Viruses” (A.M.Q. King, M.J. Adams, E.B. Carestens, E.J. Lefkowitz, eds). Elsevier-Academic Press, San Diego, CA, 1327 pp.
 
8.
Crescenzi A., Barbarossa L., Gallitelli D., Martelli G.P. 1993. Cucumber mosaic cucumovirus populations in Italy under natural epidemic conditions and after a satellite-mediated protection test. Plant Disease 77: 28–33. DOI: https://doi.org/10.1094/PD-77-....
 
9.
Dafalla G.A. 2000. Situation of tomato and pepper viruses in Africa. p. 18−24. In: “Plant Virology in Sub-Saharan Africa” (J.d’A. Hughes, B.O. Odu, eds). International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, 589 pp.
 
10.
Draeger K.R. 2016. Cucumber Mosaic. University of Wisconsin-Extension, Cooperative Extension. Available on: https://hort.uwex.edu/articles.... [Accessed: September13, 2018].
 
11.
Eni A.O., Kumar P.L., Asiedu R., Alabi O.J., Naidu R.A., Hughes J.d’A., Rey M.E.C. 2013. Characterization of Cucumber mosaic virus isolated from yam (Dioscorea spp.) in West Africa. African Journal of Biotechnology 12 (22): 3472−3480. DOI: https://doi.org/10.5897/AJB201....
 
12.
Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
 
13.
Hsu Y.-H., Wu C.-W., Lin B.-Y., Chen H.-Y., Lee M.-F., Tsai C.-H. 1995. Complete genomic RNA sequences of Cucumber mosaic virus strain NT9 from Taiwan. Archives of Virology 140: 1841−1847. DOI: https://doi.org/10.1007/BF0138....
 
14.
Hsu Y.C., Yeh T.J., Chang Y.C. 2005. A new combination of RT-PCR and reverse dot blot hybridization for rapid detection and identification of potyviruses. Journal of Virological Methods 128: 54−60. DOI: https://doi.org/10.1016/j.jvir....
 
15.
Jacquemond M. 2012. Cucumber mosaic virus. Advances in Virus Research 84: 439–504. DOI: https://doi.org/10.1016/B978-0....
 
16.
Karasawa A., Ito A., Okada I., Hase S., Ehara Y. 1997. A possible role of RNA 2 of Cucumber mosaic cucumovirus as a determinant of infection phenotype on cowpea. Japanese Journal of Phytopathology 63 (4): 289–297. DOI: https://doi.org/10.3186/jjphyt....
 
17.
Kataoka J., Masuta C., Takanami Y. 1990. Complete nucleotide sequence of RNA1 of Cucumber mosaic virus Y strain and evolutionary relationships among genome RNAs of the virus strains. Japanese Journal of Phytopathology 56 (4): 501–507. DOI: https://doi.org/10.3186/jjphyt....
 
18.
Kayode A.B., Odu B.O., Ako-Nai K.A., Alabi O.J. 2014. Occurrence of Cucumber mosaic virus subgroups IA and IB isolates in tomatoes in Nigeria. Plant Disease 98 (12): 1750. DOI: http://dx.doi.org/10.1094/PDIS....
 
19.
Kim M.-K., Kwak H.-R., Ko S.-J., Lee S.-H., Kim J.-S., Kim K.-H., Cha B.-J., Choi H.-S. 2010a. First report of Cucumber mosaic virus infecting pinewood coneflower (Rudbeckia bicolor) in Korea. Plant Pathology Journal 26: 93–98.
 
20.
Kim M.-K., Kwak H.-R., Jeong S.-G., Ko S.-J., Lee S.-H., Kim J.-S., Kim K.-H., Choi J.-K., Choi H.-S., Cha B.-J. 2010b. Characteristics of Cucumber mosaic virus infecting Zucchini in Korea. Plant Pathology Journal 26: 139–148.
 
21.
Kim M.-K., Seo J.-K., Kwak H.-R., Kim J.-S., Kim K.-H., Cha B.-J., Choi H.-S. 2014. Molecular genetic analysis of Cucumber mosaic virus populations infecting pepper suggests unique patterns of evolution in Korea. Phytopathology 104: 993–1000. DOI: http://doi.org/10.1094/PHYTO-1....
 
22.
Leke W.N., Mignouna D.B., Brown J.K., Kvarnheden A. 2015. Begomovirus disease complex: Emerging threat to vegetable production systems of West and Central Africa. Agriculture and Food Security 4 (1): 1. DOI: https://doi.org/10.1186/s40066....
 
23.
Liu Y.Y., Yu S.L., Lan Y.F., Zhang C.L., Hou S.S., Li X.D., Chen X.Z., Zhu X.P. 2009. Molecular variability of five Cucumber mosaic virus isolates from China. Acta Virologica 53: 89–97.
 
24.
Makinde E.A., Ayoola O.T., Makinde E.A. 2009. Intercropping leafy greens and maize on weed infestation, crop development, and yield. International Journal of Vegetable Science 15 (4): 402−411. DOI: https://doi.org/10.1080/193152....
 
25.
Moyle R., Pretorius L.-S., Shuey L.S., Nowak E., Schenk P.M. 2018. Analysis of the complete genome sequence of Cucumber mosaic virus strain K. Genome Announcement 6 (7): e00053-18. DOI: https://doi.org/10.1128/genome....
 
26.
Odedara O.O., Kumar P.L. 2017. Incidence and diversity of viruses in cowpeas and weeds in the unmanaged farming systems of savanna zones in Nigeria. Archives of Phytopathology and Plant Protection 50: 1−12. DOI: https://doi.org/10.1080/ 03235408.2016.1241203.
 
27.
Ohshima K., Matsumoto K., Yasaka R., Nishiyama M., Soejima K., Korkmaz S., Ho S.Y.W., Gibbs A.J., Takeshita M. 2016. Temporal analysis of reassortment and molecular evolution of Cucumber mosaic virus: Extra clues from its segmented genome. Virology 487: 188–197. DOI: https://doi.org/10.1016/j.viro....
 
28.
Owolabi A.T., Rabenstein F., Taiwo M.A., Ehrigh F., Maiss E. 2011. Characterization and identification of a potyvirus causing mosaic disease of Cucurbita moschata Duch Ex. Poir in Calabar, South East Nigeria. International Journal of Plant Pathology 2: 165–176. DOI: https://doi.org/10.3923/ijpp.2....
 
29.
Palukaitis P., García-Arenal F. 2003. Cucumoviruses. Advances in Virus Research 62: 241–323. DOI: https://doi.org/10.1016/S0065-....
 
30.
Phan M.S.V., Seo J.-K., Choi H.-S., Lee S.-H., Kim K.-H. 2014. Molecular and biological characterization of an isolate of Cucumber mosaic virus from Glycine soja by generating its infectious full-genome cDNA clones. Plant Pathology Journal 30: 159–167. DOI: http://doi.org/10.5423/PPJ.OA.....
 
31.
Rabie M., Ratti C., Calassanzio M., Aleem A.E., Fattouh F.A. 2017. Phylogeny of Egyptian isolates of Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV) infecting Solanum lycopersicum. European Journal of Plant Pathology 149 (1): 219–225. DOI: https://doi.org/10.1007/s10658....
 
32.
Rezaian M.A., Williams R.H., Symons R.H. 1985. Nucleotide sequence of Cucumber mosaic virus RNA 1: Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. European Journal of Biochemistry 150 (2): 331–339. DOI: https://doi.org/10.1111/j.1432....
 
33.
Rizzo T.M., Palukaitis P. 1989. Nucleotide sequence and evolutionary relationships of Cucumber mosaic virus (CMV) strains: CMV RNA 1. Journal of General Virology 70: 1–11. DOI: https://doi.org/10.1099/0022-1....
 
34.
Roossinck M.J. 2002. Evolutionary history of Cucumber mosaic virus deduced by phylogenetic analyses. Journal of Virology 76 (7): 3382–3387. DOI: https://doi.org/10.1128/JVI.76....
 
35.
Salem N.M., Ehlers J.D., Roberts P.A., Ng J.C.K. 2010. Biological and molecular diagnosis of seedborne viruses in cowpea germplasm of geographically diverse sub‐Saharanorigins. Plant Pathology 59 (4): 773–784. DOI: https://doi.org/10.1111/j.1365....
 
36.
Shoyinka S.A., Brunt A.A., Phillips S., Lesemann D.E., Thottappilly G., Lastra R. 1987. The occurrence, properties and affinities of Telfairia mosaic virus, a potyvirus prevalent in Telfairia occidentalis (Cucurbitaceae) in South Western Nigeria. Journal of Phytopathology 119 (1): 13–24.
 
37.
Szilassy D., Salánki K., Balázs E. 1999. Molecular evidence for the existence of two distinct subgroups in cucumber mosaic cucumovirus. Virus Genes 18 (3): 221–227.
 
38.
Tamarzizt H.B., Montarry J., Girardot G., Fakhfakh H., Tepfer M., Jacquemond M. 2013. Cucumber mosaic virus populations in Tunisian pepper crops are mainly composed of virus reassortants with resistance‐breaking properties. Plant Pathology 62 (6): 1415–1428. DOI: https://doi.org/10.1111/ppa.12....
 
39.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. DOI: https://doi.org/10.1093/molbev....
 
40.
Tepfer M., Girardot G., Fénéant L., Tamarzizt B.H., Verdin E., Moury B., Jacquemond M. 2016. A genetically novel, narrow-host-range isolate of Cucumber mosaic virus (CMV) from rosemary. Archives of Virology 161 (7): 2013–2017. DOI: https://doi.org/10.1007/s00705....
 
41.
Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680.
 
42.
Thompson J.R., Langenhan J.L., Fuchs M., Perry K.L. 2015. Genotyping of Cucumber mosaic virus isolates in western New York State during epidemic years: Characterization of an emergent plant virus population. Virus Research 210: 169–177. DOI: https://doi.org/10.1016/j.viru....
 
43.
Wang H.Y., Zhang H.R., Du Z.Y., Zeng R., Chen J. 2009. Evolutionary characterization of two Cucumber mosaic virus isolates infecting Pinellia ternata of Araceae family. Available on: http://www.paper.edu.cn/index.....
 
44.
Waterworth H.E., Povish W.R. 1975. A virus related to CMV isolated from imported Ixora plants. Phytopathology 65: 728–729. DOI: https://doi.org/10.1094/Phyto-....
 
45.
Wyatt S.D., Brown J.K. 1996. Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86: 1288–1293. DOI: https://doi.org/10.1094/Phyto-....
 
46.
Wylie S., Wilson C.R., Jones R.A.C., Jones M.G.K. 1993. A polymerase chain reaction assay for Cucumber mosaic virus in lupin seeds. Australian Journal of Agricultural Research 44: 41–51. DOI: https://doi.org/10.1071/AR9930....
 
47.
Zeng R., Chen Y.-F., Yan S.-J, Li D.-J., Chen J.-S. 2008. Complete sequence analysis of Cucumber mosaic virus CTL Isolate. Acta Horticulturae Sinica 35 (2): 213–220.
 
48.
Zitter T.A., Murphy J.F. 2009. Cucumber mosaic virus. The Plant Health Instructor. American Phytopathological Society. Available on: http://www.apsnet.org/edcenter... /viruses/Pages/Cucumbermosaic.aspx. [Accessed: August 19, 2018]. DOI: https://doi.org/10.1094/PHI-I-....
 
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